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A case of mistaken identity

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Figure 1: Modes of misidentification of sequence elements.

References

  1. Schlub, T.E., Smyth, R.P., Grimm, A.J., Mak, J. & Davenport, M.P. PLoS Comput. Biol. 6, e1000766 (2010).

    Article  Google Scholar 

  2. Hill, A.J. et al. Nat. Methods 15, 271–274 (2018).

    Article  CAS  Google Scholar 

  3. Sack, L.M., Davoli, T., Xu, Q., Li, M.Z. & Elledge, S.J. G3 (Bethesda) 6, 2781–2790 (2016).

    Article  CAS  Google Scholar 

  4. Patwardhan, R.P. et al. Nat. Biotechnol. 27, 1173–1175 (2009).

    Article  CAS  Google Scholar 

  5. Adamson, B. et al. Cell 167, 1867–1882.e21 (2016).

    Article  CAS  Google Scholar 

  6. Datlinger, P. et al. Nat. Methods 14, 297–301 (2017).

    Article  CAS  Google Scholar 

  7. Xie, S., Duan, J., Li, B., Zhou, P. & Hon, G.C. Mol. Cell 66, 285–299.e5 (2017).

    Article  CAS  Google Scholar 

  8. Dixit, A. et al. Cell 167, 1853–1866.e17 (2016).

    Article  CAS  Google Scholar 

  9. Han, K. et al. Nat. Biotechnol. 35, 463–474 (2017).

    Article  CAS  Google Scholar 

  10. Shen, J.P. et al. Nat. Methods 14, 573–576 (2017).

    Article  CAS  Google Scholar 

  11. Najm, F.J. et al. Nat. Biotechnol. 36, 179–189 (2018).

    Article  CAS  Google Scholar 

  12. Boettcher, M. et al. Nat. Biotechnol. 36, 170–178 (2018).

    Article  CAS  Google Scholar 

  13. Gasperini, M. et al. Am. J. Hum. Genet. 101, 192–205 (2017).

    Article  CAS  Google Scholar 

  14. Du, D. et al. Nat. Methods 14, 577–580 (2017).

    Article  CAS  Google Scholar 

  15. Michlits, G. et al. Nat. Methods 14, 1191–1197 (2017).

    Article  CAS  Google Scholar 

  16. Feldman, D., Singh, A., Garrity, A.J. & Blainey, P.C. Preprint at bioRxiv https://doi.org/10.1101/262121 (2018).

  17. Lenz, T.L. & Becker, S. Gene 427, 117–123 (2008).

    Article  CAS  Google Scholar 

  18. Thompson, J.R., Marcelino, L.A. & Polz, M.F. Nucleic Acids Res. 30, 2083–2088 (2002).

    Article  CAS  Google Scholar 

  19. Meyerhans, A., Vartanian, J.P. & Wain-Hobson, S. Nucleic Acids Res. 18, 1687–1691 (1990).

    Article  CAS  Google Scholar 

  20. Odelberg, S.J., Weiss, R.B., Hata, A. & White, R. Nucleic Acids Res. 23, 2049–2057 (1995).

    Article  CAS  Google Scholar 

  21. Pääbo, S., Irwin, D.M. & Wilson, A.C. J. Biol. Chem. 265, 4718–4721 (1990).

    PubMed  Google Scholar 

  22. Hegde, M., Strand, C., Hanna, R.E. & Doench, J.G. PLoS One 13, e0197547 (2018).

    Article  Google Scholar 

  23. Judo, M.S., Wedel, A.B. & Wilson, C. Nucleic Acids Res. 26, 1819–1825 (1998).

    Article  CAS  Google Scholar 

  24. Zylstra, P., Rothenfluh, H.S., Weiller, G.F., Blanden, R.V. & Steele, E.J. Immunol. Cell Biol. 76, 395–405 (1998).

    Article  CAS  Google Scholar 

  25. Sinha, R. et al. Preprint at bioRxiv https://doi.org/10.1101/125724 (2017).

  26. Griffiths, J.A., Richard, A.C., Bach, K., Lun, A.T.L. & Marioni, J.C. Nat. Commun. https://doi.org/10.1038/s41467-018-05083-x (2018).

  27. Costello, M. et al. BMC Genomics 19, 332 (2018).

    Article  Google Scholar 

  28. Kircher, M., Sawyer, S. & Meyer, M. Nucleic Acids Res. 40, e3 (2012).

    Article  CAS  Google Scholar 

  29. MacConaill, L.E. et al. BMC Genomics 19, 30 (2018).

    Article  Google Scholar 

  30. Larsson, A.J.M., Stanley, G., Sinha, R., Weissman, I.L. & Sandberg, R. Nat. Methods 15, 305–307 (2018).

    Article  CAS  Google Scholar 

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Hanna, R., Doench, J. A case of mistaken identity. Nat Biotechnol 36, 802–804 (2018). https://doi.org/10.1038/nbt.4208

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