Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

One flexible loop in OST lassos both substrates

The crystal structure of an oligosaccharyltransferase in complex with a sugar donor and an acceptor peptide provides insight into the mechanism of protein glycosylation and reveals how lipid-linked oligosaccharides are positioned in the enzyme active site.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: EL5 clamps both substrates into the OST active site.

Accession codes

Accessions

Protein Data Bank

References

  1. Napiórkowska, M. et al. Nat. Struct. Mol. Biol. 24, 1100–1106 (2017).

    Article  Google Scholar 

  2. Gerber, S. et al. J. Biol. Chem. 288, 8849–8861 (2013).

    Article  CAS  Google Scholar 

  3. Lizak, C. et al. Nat. Commun. 4, 2627 (2013).

    Article  Google Scholar 

  4. Liu, F. et al. J. Am. Chem. Soc. 136, 566–569 (2014).

    Article  CAS  Google Scholar 

  5. Lizak, C., Gerber, S., Numao, S., Aebi, M. & Locher, K.P. Nature 474, 350–355 (2011).

    Article  CAS  Google Scholar 

  6. Matsumoto, S. et al. Proc. Natl. Acad. Sci. USA 110, 17868–17873 (2013).

    Article  CAS  Google Scholar 

  7. Igura, M. et al. EMBO J. 27, 234–243 (2008).

    Article  CAS  Google Scholar 

  8. Jaffee, M.B. & Imperiali, B. Biochemistry 50, 7557–7567 (2011).

    Article  CAS  Google Scholar 

  9. Kelleher, D.J., Karaoglu, D., Mandon, E.C. & Gilmore, R. Mol. Cell 12, 101–111 (2003).

    Article  CAS  Google Scholar 

  10. Karaoglu, D., Kelleher, D.J. & Gilmore, R. Biochemistry 40, 12193–12206 (2001).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Reid Gilmore.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shrimal, S., Cherepanova, N. & Gilmore, R. One flexible loop in OST lassos both substrates. Nat Struct Mol Biol 24, 1009–1010 (2017). https://doi.org/10.1038/nsmb.3508

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsmb.3508

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing