Abstract
To control the release of insulin in response to the concentration of glucose, a glucose–responsive polymer membrane was designed by combining a glucose oxidase (GOD) immobilized membrane, a sensor for glucose, with a poly(amine) membrane which regulates the permeation rate of insulin. The permeability of insulin was increased by the addition of glucose. Gluconic acid produced by an enzymatic reaction between GOD and glucose induced a decrease in the pH value of the medium. This caused the protonation of tertiary amino groups in the membrane resulting in an increase in the water content of the poly(amine) membrane. The permeability of insulin through a complex membrane thus increases with glucose concentration.
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Ishihara, K., Kobayashi, M., Ishimaru, N. et al. Glucose Induced Permeation Control of Insulin through a Complex Membrane Consisting of Immobilized Glucose Oxidase and a Poly(amine). Polym J 16, 625–631 (1984). https://doi.org/10.1295/polymj.16.625
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DOI: https://doi.org/10.1295/polymj.16.625
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