Abstract
Pentapeptide Tyr-Ile-Gly-Ser-Arg (YIGSR), an amino acid sequence existing in the cell attachment domain of the B1 chain of laminin, was synthesized by an improved liquid-phase procedure. Bioactivity of YIGSR as cell recognition determinant was investigated using L-929 fibroblast cell and A431 epidermoid cell. The YIGSR pentapeptide was immobilized to ethylene-acrylic acid copolymer (PEA) film, and the number of cells attached to the immobilized film was counted. In addition, the cell-attachment activity of YIGSR toward the cells was also evaluated by the cell inhibition test. These two tests led to a conclusion that YIGSR acts as a cell determinant toward L-929 and A431 cells.
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Hirano, Y., Okuno, M., Hayashi, T. et al. Liquid-Phase Synthesis and Cell Attachment Activity of Bioactive Pentapeptide YIGSR. Polym J 24, 465–472 (1992). https://doi.org/10.1295/polymj.24.465
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DOI: https://doi.org/10.1295/polymj.24.465