Abstract
Porous poly(γ-methyl L-glutamate) spherical particles underwent transition α-helix-to-β-structure in the main chain as a result of heat treatment in water or methanol. The particles obtained maintained their sphericity and characteristic porosity after the heat treatment, while the transition increased insolubility against organic solvents. The amount of β-structure depended on the temperature. The transition in water included hydrolysis of the residual carbomethoxy groups. Adsorption capacity of the particles for cytochrome C depended remarkably on the content of β-structure as well as the amount of carboxyl groups produced.
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The estrification of partially hydrolized PMLG particles was performed as follows: 5 wet-ml of the particles with 0.91 mmol g−1 of carboxyl groups were suspended in 100 ml of methanol containing of 45 mg of sulfuric acid. The mixture was stirred at 60°C for 48 h, then the complete disappearence of carboxyl groups was confirmed by IR spectroscopy.
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Hirayama, C., Ihara, H., Ishimara, SI. et al. Preparation of Porous Spherical Poly(γ-methyl L-glutamate) Particles Containing β-Structure. Polym J 26, 61–66 (1994). https://doi.org/10.1295/polymj.26.61
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DOI: https://doi.org/10.1295/polymj.26.61