Abstract
The effects of sodium poly(styrene sulfonate) on an antibody production and cell growth of a hybridoma cell were studied. Although sodium poly(styrene sulfonate) inhibited the antibody production and cell growth at lower concentration with increasing molecular weight in serum-free cultures, sodium poly(styrene sulfonate)s with medium weight-average molecular weights (Mw) such as 35000 and 18000 suppressed antibody production without inhibiting cell growth at appropriate concentrations. Sodium poly(styrene sulfonate) (Mw=35000) decreased the extracellular concentration of a μ heavy chain forming an antibody, while it had no effect on an intracellular antibody or μ heavy chain concentration. This suggests that the sodium poly(styrene sulfonate) suppresses secreting the antibody assembled by μ heavy chains and λ light chains out of the cell without inhibiting the synthesis and association of both chains in the cell.
Similar content being viewed by others
Article PDF
References
M. M. Yamamoto, Y. Mori, K. Osada, and H. Murakami, Biosci. Biotech. Biochem., in press.
M. Maeda, H. Murakami, H. Ohta, and M. Tajima, Biosci. Biotech. Biochem., 56, 427 (1992).
T. C. Merigan, Nature, 214, 416 (1967).
L. G. Donaruma, R. M. Ottenbrite, and O. Vogel, Ed., “Anionic Polymeric Drugs,” John Wiley and Sons, New York, N.Y., 1980.
R. M. Ottenbrite and G. B. Butler, Ed., “Anticancer and Interferon Agents,” Marcel Decker, Inc., New York, N.Y., 1984.
T. Sato, K. Kojima, T. Ihda, J. Sunamoto, and R. M. Ottenbrite, J. Bioactive Compatible Polym., 1, 448 (1986).
Y. Suda, H. Yamamoto, M. Sumi, N. Oku, F. Ito, and R. M. Ottenbrite, J. Bioactive Compatible Polym., 7, 15 (1992).
Y. Suda, S. Kusumoto, N. Oku, H. Yamamoto, M. Sumi, F. Ito, and R. M. Ottenbrite, J. Bioactive Compatible Polym., 7, 275 (1992).
K. Hatanaka, T. Ohtsuki, and M. Kunou, Chem. Lett., 1407 (1994).
T. Matsuda, Y. Nakayama, Y. Tutumi, and T. Mayumi, Polym. Prepr., Jpn., 41, 3301 (1992).
K. Park and J. R. Robinson, Int. J. Pharm., 19, 107 (1984).
H. Murakami, S. Hashizume, H. Ohashi, K. Shinohara, K. Yasumoto, K. Nomoto, and H. Omura, In Vitro Cell. Develop. Biol., 21, 593 (1985).
H. Murakami, H. Masui, G. H. Sato, N. Sueoka, T. P. Chow, and T. Kano-Sueoka, Proc. Natl. Acad. Sci. U.S.A., 79, 1158 (1982).
K. Yamada, K. Akiyoshi, H. Murakami, T. Sugahara, I. Ikeda, K. Toyoda, and H. Omura, In Vitro Cell. Develop. Biol., 25, 243 (1989).
E. K. Leammli, Nature, 227, 680 (1970).
H. Towbin, T. Staehelin, and J. Gordon, Proc. Natl. Acad. Sci. U.S.A., 76, 4350 (1979).
H. Tachibana, S. Shirahata, and H. Murakami, Biochem. Biophys. Res. Commun., 189, 625 (1992).
R. Richman and R. F. Flinckeringer, Life Sci., 35, 911 (1984).
D. G. Bole, L. M. Hendershot, and J. F. Kearney, J. Cell Biol., 102, 1558 (1986).
L. M. Hendershot, J. Cell Biol., 111, 829 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nagira, K., Hayashida, M., Shiga, M. et al. Effects of Sodium Poly(styrene sulfonate) on Antibody Production and Cell Growth of HB4C5 Hybridoma Cells. Polym J 27, 719–727 (1995). https://doi.org/10.1295/polymj.27.719
Issue Date:
DOI: https://doi.org/10.1295/polymj.27.719