Abstract
Five sodium salt samples of hyaluronic acid ranging in weight-average molecular weight Mw from 1.9×105 to 1.5×106 have been studied by light scattering and viscometry in aqueous sodium chloride at 25°C. The intrinsic viscosity ([η]) data obtained at 10 different NaCl concentrations Cs covering a range from 0.005 to 2.5 M are analyzed along with previous data for lower Mw on the basis of the Yamakawa-Fujii-Yoshizaki theory for unperturbed wormlike chains combined with the quasi-two-parameter (QTP) theory for excluded-volume effects. Fairly satisfactory agreement is observed between theory and experiment at Cs higher than 0.01 M over the range of molecular weight from 3.8×103 to 1.5×106, though the degree of agreement tends to lower with decreasing Cs. When the wormlike chain parameters and the excluded-volume strength estimated from [η] are used, the QTP theory with the known expression for 〈S2〉 (the mean-square radius of gyration) of the unperturbed wormlike chain is found to describe almost quantitatively the measured 〈S2〉 for Na hyaluronate at Cs=0.02, 0.1, and 0.5 M. Thus, it is concluded that this theory for nonionic chains is applicable, in a fairly good approximation, to 〈S2〉 and [η] of the charged polysaccharide in aqueous NaCl unless Cs is lower than 0.02 M.
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See, for fiexible polymers, F. Abe, Y. Einaga, T. Yoshizaki, and H. Yamakawa, Macromolecules, 26, 1884 (1993).
K. Horita, F. Abe, Y. Einaga, and H. Yamakawa, Macromolecules, 26, 5067 (1993).
F. Abe, K. Horita, Y. Einaga, and H. Yamakawa, Macromolecules, 27, 725 (1994).
M. Kamijo, F. Abe, Y. Einaga, and H. Yamakawa, Macromolecules, 28, 1095 (1995).
See, for stiff chains, T. Norisuye, A. Tsuboi, and A. Teramoto, Polym. J., 28, 357 (1996).
H. Yamakawa, “Helical Wormlike Chains in Polymer Solutions,” Springer, Berlin, 1997.
O. Kratky and G. Porod, Rec. Trav. Chim., 68, 1106 (1949).
K. Hayashi, K. Tsutsumi, F. Nakajima, T. Norisuye, and A. Teramoto, Macromolecules, 28, 3824 (1995).
K. Hayashi, K. Tsutsumi, T. Norisuye, and A. Teramoto, Polym. J., 28, 922 (1996).
H. Yamakawa and M. Fujii, Macromolecules, 7, 128 (1974).
H. Yamakawa and T. Yoshizaki, Macromolecules, 13, 633 (1980).
Y. Nakanishi, T. Norisuye, A. Teramoto, and S. Kitamura, Macromolecules, 26, 4220 (1993).
H. Fujita, “Polymer Solutions,” Elsevier, Amsterdam, 1990.
H. Yamakawa and W. H. Stockmayer, J. Chem. Phys., 57, 2843 (1972).
J. Shimada and H. Yamakawa, J. Chem. Phys., 85, 591 (1986).
A. J. Barrett, Macromolecules, 17, 1566 (1984).
T. Yoshizaki, I. Nitta, and H. Yamakawa, Macromolecules, 21, 165 (1988).
R. L. Cleland, Biopolymers, 23, 647 (1984).
T. Odijk, J. Polym. Sci., Polym. Phys. Ed., 15, 477 (1977).
J. Skolnick and M. Fixman, Macromolecules, 10, 944 (1977).
H. Benoit and P. Doty, J. Phys. Chem., 57, 958 (1953).
C. Domb and A. J. Barrett, Polymer, 17, 179 (1976).
N. Mizukoshi and T. Norisuye, to be published.
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Tsutsumi, K., Norisuye, T. Excluded-Volume Effects in Sodium Hyaluronate Solutions Revisited. Polym J 30, 345–349 (1998). https://doi.org/10.1295/polymj.30.345
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DOI: https://doi.org/10.1295/polymj.30.345