Abstract
A lipid-coated glycoside hydrolase was prepared in which the enzyme surface is covered with a lipid monolayer and two long alkyl tails serve to solubilize the enzyme in organic solvents. In the two-phase aqueous-organic system, the lipid-coated enzyme exists in the organic phase and acts as an efficient catalyst for transglycosylation of various lipophilic dialkyl alcohols with oligosaccharides in the aqueous buffer solution. When a native glycoside hydrolase was employed in the two-phase system, neither the transgalactosylation nor the hydrolysis reaction proceeded due to the denaturation of the enzyme at the interface. Effects of chemical structures of acceptor alcohols on the transglycosylation catalyzed by the lipid-coated enzyme were also studied.
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Mori, T., Fukusho, s., Kojima, J. et al. Enzymatic Syntheses of Glycolipids Catalyzed by a Lipid-coated Glycoside Hydrolase in the Organic-Aqueous Two Phase System. Polym J 31, 1105–1108 (1999). https://doi.org/10.1295/polymj.31.1105
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DOI: https://doi.org/10.1295/polymj.31.1105