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Microbial prospection of an Amazonian blackwater lake and whole-genome sequencing of bacteria capable of polyhydroxyalkanoate synthesis

Polymer Journal volume 53, pages 191–202 (2021)Cite this article

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Abstract

Biopolymers are driving the plastic industry to the next generation of environmentally friendly bioproducts, considering green chemistry principles and contemporary economic concepts, such as environmental, social, and governance (ESG) criteria. Hence, microbial biopolymers arise in this context. Resulting from a natural carbon and energy storage process, polyhydroxyalkanoates are the raw material for a range of products based on plastic, with the advantage of being biodegradable in a short period of time. Discovering new biopolymers with different properties, carbon sources and PHA-related enzymes will facilitate market development as well as competition with petrochemical polymers. This work reports the experimental findings of PHA production and genomic data for two bacteria, Ralstonia pickettii and Aquitalea sp., isolated from a blackwater lake located in the ecological reserve of Tupé, Iranduba, AM, Brazil. They were able to produce PHB from carbon sources related to sugar, and R. pickettii also produced PHB from soybean oil and lignin derivatives. Whole-genome sequencing of these isolates enabled the identification of the genetic background to use other oxidizable carbon sources, such as lactic and malonic acids, amino acids, and lignin.

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Acknowledgements

This work was financially supported by MCTIC/PROPESP/UFAM 041/2016 through a grant for AJ Mota and also by JST ERATO (grant number JPMJER1602). We are grateful for the financial and logistical support provided by RIKEN through a grant for LM Castro. We also thank Cheris Williams for revising the English grammar.

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  1. Laboratório de Biodegradação, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Av. Rodrigo Otávio, 6200, Coroado I, 69080-900, Manaus, AM, Brazil

    Lorena M. Castro & Adolfo J. Mota

  2. Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto, Japan

    Choon Pin Foong & Keiji Numata

  3. Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, Hirosawa, Wako, Saitama, Japan

    Mieko Higuchi-Takeuchi, Kumiko Morisaki & Keiji Numata

  4. Instituto de Saúde e Biotecnologia de Coari, Universidade Federal do Amazonas, Urucú, 69050-570, Coari, AM, Brazil

    Eraldo F. Lopes

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Castro, L.M., Foong, C.P., Higuchi-Takeuchi, M. et al. Microbial prospection of an Amazonian blackwater lake and whole-genome sequencing of bacteria capable of polyhydroxyalkanoate synthesis. Polym J 53, 191–202 (2021). https://doi.org/10.1038/s41428-020-00424-4

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