Abstract
The cellular scaffold plays a crucial role in regulating cellular behavior, and controlling its mechanical properties is important in the field of regenerative medicine. In this study, common silicone oil was employed as the cellular scaffold and coated with a polymeric ultrathin film at the oil/water interface. Inspired by mussel adhesion, polydopamine ultrathin films were formed at the silicone oil/water interface even in neutral solution. The MCF-7 cells successfully adhered to the oil/water interface without aggregation during cell growth. Interfacial wrinkles were induced by changes in the oil volume and the compressive stress, and the MCF-7 cells adhered to the oil/water interface and were arranged along the wrinkles. These polydopamine interfacial films provide new opportunities for investigating the relationships between toughness and patterns for tissue engineering and regenerative medicine.
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Acknowledgements
We thank Dr. Akichika Kumatani, Tohoku University, for assisting with the AFM, and Dr. Yuta Nakayasu, Tohoku University, for assisting with the XPS analyses. We also thank Prof. Joseph Paulsen and Dr. Mengfei He, Syracuse University, and Prof. Vincent Démery, École Supérieure de Physique et Chimie Industrielles de Paris, for discussing wrinkle formation. This research is supported by the KAKENHI from JSPS (nos. 19K15598, 22K14579, and 22H05397), ACT-X from JST (no. JPMJAX22K1), Research Institutes Ensemble Grant for young researchers, and FRIS Creative Interdisciplinary Collaboration Program from Tohoku University for financial support the Cooperative Research Project of Research Center for Biomedical Engineering.
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Abe, H., Ina, T., Kaji, H. et al. Mussel-inspired interfacial ultrathin films for cellular adhesion on the wrinkled surfaces of hydrophobic fluids. Polym J 55, 1231–1236 (2023). https://doi.org/10.1038/s41428-023-00799-0
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DOI: https://doi.org/10.1038/s41428-023-00799-0