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A new α-pyrone from Arthrinium pseudosinense culture medium and its estrogenic activity in MCF-7 cells

The Journal of Antibiotics volume 74, pages 893–897 (2021)Cite this article

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Abstract

A new α-pyrone analog, arthrifuranone A (1) was isolated from an EtOAc-extract of Arthrinium pseudosinense culture medium. The isolation workflow was guided by a Molecular Networking-based dereplication strategy. The chemical structure of the new compound was elucidated using MS and NMR spectroscopic techniques, and the absolute configuration was established by the Mosher’s method and gauge-including atomic orbital NMR chemical shift calculations, followed by DP4 + analysis. The isolated compound was evaluated for its estrogenic activity using the MCF-7 estrogen responsive human breast cancer cells. Compound 1 showed estrogenic activity by increasing the proliferation of MCF-7 cells at the concentration of 3.125 μM via phosphorylation of estrogen receptor-α.

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Acknowledgements

This research was funded by Korea University, Korea Polar Research Institute (Grant: PE21150), and the National Research Foundation of Korea (NRF-2019R1A2C1006226 and NRF-2019R1A4A1020626).

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Author notes

  1. These authors contributed equally: Haeun Kwon, Quynh Nhu Nguyen

Authors and Affiliations

  1. Department of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea

    Haeun Kwon & Dongho Lee

  2. College of Korean Medicine, Gachon University, Seongnam, Republic of Korea

    Quynh Nhu Nguyen & Ki Sung Kang

  3. School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea

    Myung Woo Na & Ki Hyun Kim

  4. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, People’s Republic of China

    Yuanqiang Guo

  5. Korea Polar Research Institute, Korea Ocean Research and Development Institute, Incheon, Republic of Korea

    Joung Han Yim

  6. Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, Republic of Korea

    Sang Hee Shim

  7. Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea

    Jae-Jin Kim

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  1. Haeun Kwon
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Contributions

HK and QNN performed the experiments, with the assistance of MWN, KHK, YG, and SHS. JJK offered the fungal source. DL and KSK designed the research. HK, QNN, DL, and KSK wrote the paper.

Corresponding authors

Correspondence to Ki Sung Kang or Dongho Lee.

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Kwon, H., Nguyen, Q.N., Na, M.W. et al. A new α-pyrone from Arthrinium pseudosinense culture medium and its estrogenic activity in MCF-7 cells. J Antibiot 74, 893–897 (2021). https://doi.org/10.1038/s41429-021-00473-8

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