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Role and the molecular mechanism of lncRNA PTENP1 in regulating the proliferation and invasion of cervical cancer cells

Gene Therapy volume 29, pages 464–475 (2022)Cite this article

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Abstract

Cervical cancer ranks second in the major causes of cancer-relevant death in female population worldwide. It is extensively reported that lncRNAs are implicated in biological activities of diverse cancers. LncRNA PTENP1 has been recently reported as a tumor suppressor in several malignancies. However, the pathophysiological function and the potential regulatory mechanism of PTENP1 in cervical cancer have never been studied. In this research, PTENP1 was pronouncedly downregulated in cervical cancer tissues, and low PTENP1 level was tightly linked to advanced stage and poor prognosis in cervical cancer. Overexpressing PTENP1 inhibited cervical cancer progression by suppressing cell growth, motility and epithelial-to-mesenchymal transition (EMT). PTENP1 was confirmed to decoy miR-27a-3p to upregulate EGR1 expression in cervical cancer cells. Additionally, EGR1 knockdown reversed the repressive effect of PTENP1 overexpression on cervical cancer progression. In a word, current study was the first to uncover the biological functions of PTENP1 as well as its modulatory mechanism in cervical cancer, which may offer a new potent target for treating patients with cervical cancer.

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Fig. 1: PTENP1 is predominantly downregulated in cervical cancer.
Fig. 2: PTENP1 overexpression inhibits cell proliferation and facilitates cell apoptosis in cervical cancer.
Fig. 3: PTENP1 overexpression represses cell migration and invasion and EMT in cervical cancer.
Fig. 4: PTENP1 sponges miR-27a-3p in cervical cancer.
Fig. 5: PTENP1 sponges miR-27a-3p to regulate the expression of EGR1 in cervical cancer.
Fig. 6: EGR1 knockdown rescues the inhibiting impacts of PTENP1 overexpression on cervical cancer cells.

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Funding

This study was funded by Luoyang Science and Technology Key Project (No. 1503006A-6).

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  1. Reproductive Center, Luoyang Central Hospital Affiliated to Zhengzhou University, No. 288 Zhongzhou Road, 471000, Luoyang, Henan, China

    Chengping Wu & Fang Wang

  2. Reproductive Center, the Second Affiliated Hospital of Zhengzhou University, No. 2 Jingba Road, 450014, Zhengzhou, Henan, China

    Li Tan

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Wu, C., Wang, F. & Tan, L. Role and the molecular mechanism of lncRNA PTENP1 in regulating the proliferation and invasion of cervical cancer cells. Gene Ther 29, 464–475 (2022). https://doi.org/10.1038/s41434-020-00189-8

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