Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Matters Arising
  • Published:

Reply to: Lactate measurements in an integrated perfusion machine for human livers

The Original Article was published on 26 October 2020

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Lactate clearance during ex vivo perfusion.

Data availability

Data from this study will be made available upon reasonable request to the corresponding author.

References

  1. Stephenson, B. T. F., Afford, S. C., Mergental, H. & Mirza, D. F. Lactate measurements in an integrated perfusion machine for human livers. Nat. Biotechnol. https://doi.org/10.1038/s41587-020-0626-9 (2020).

  2. Eshmuminov, D. et al. An integrated perfusion machine preserves injured human livers for 1 week. Nat. Biotechnol. 38, 189–198 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Rashkin, M. C., Bosken, C. & Baughman, R. P. Oxygen delivery in critically ill patients. Relationship to blood lactate and survival. Chest 87, 580–584 (1985).

    Article  CAS  PubMed  Google Scholar 

  4. Vincent, J. L., Quintairos, E. S. A., Couto, L. Jr. & Taccone, F. S. The value of blood lactate kinetics in critically ill patients: a systematic review. Crit. Care 20, 257 (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  5. Schroeder, T. H. & Hansen, M. Effects of fresh versus old stored blood in the priming solution on whole blood lactate levels during paediatric cardiac surgery. Perfusion 20, 17–19 (2005).

    Article  PubMed  Google Scholar 

  6. Prudent, M. et al. Small-scale perfusion bioreactor of red blood cells for dynamic studies of cellular pathways: proof-of-concept. Front Mol. Biosci. 3, 11 (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  7. Watson, C. J. E. et al. Observations on the ex situ perfusion of livers for transplantation. Am. J. Transplant. 18, 2005–2020 (2018).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Vogel, T. et al. The 24-hour normothermic machine perfusion of discarded human liver grafts. Liver Transpl. 23, 207–220 (2017).

    Article  PubMed  Google Scholar 

  9. Bernal, W., Donaldson, N., Wyncoll, D. & Wendon, J. Blood lactate as an early predictor of outcome in paracetamol-induced acute liver failure: a cohort study. Lancet 359, 558–563 (2002).

    Article  CAS  PubMed  Google Scholar 

  10. Bernal, W. & Wendon, J. Acute liver failure. N. Engl. J. Med. 370, 1170–1171 (2014).

    Article  CAS  PubMed  Google Scholar 

  11. Chiolero, R. et al. Effect of major hepatectomy on glucose and lactate metabolism. Ann. Surg. 229, 505–513 (1999).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Sohlenius-Sternbeck, A. K. Determination of the hepatocellularity number for human, dog, rabbit, rat and mouse livers from protein concentration measurements. Toxicol. In Vitro 20, 1582–1586 (2006).

    Article  CAS  PubMed  Google Scholar 

  13. Mergental, H. et al. Transplantation of declined liver allografts following normothermic ex-situ evaluation. Am. J. Transplant. 16, 3235–3245 (2016).

    Article  CAS  PubMed  Google Scholar 

  14. Chouchani, E. T. et al. A unifying mechanism for mitochondrial superoxide production during ischemia-reperfusion injury. Cell Metab. 23, 254–263 (2016).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Contributions

P.D., M.M., D.E., D.B., L.B.B., M.H., M.J.S., P.R.v.R. and P.-A.C. designed the perfusion machine and established the perfusion protocol, performed the perfusion experiments, generated and interpreted the data, and wrote the manuscript.

Corresponding author

Correspondence to Pierre-Alain Clavien.

Ethics declarations

Competing interests

The University of Zurich and the Swiss Federal Institute of Technology in Zurich (ETH) (P.D., D.E., D.B., L.B.B., M.H., M.J.S., P.R.v.R. and P.-A.C.) have applied for a patent on this new perfusion technology (PCT/EP2019/051252).

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dutkowski, P., Mueller, M., Eshmuminov, D. et al. Reply to: Lactate measurements in an integrated perfusion machine for human livers. Nat Biotechnol 38, 1263–1264 (2020). https://doi.org/10.1038/s41587-020-0627-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41587-020-0627-8

Search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research