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Lipid nanoparticle-enabled gene editing in the lung via inhalation

Nature Biotechnology volume 41, pages 1394–1395 (2023)Cite this article

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The ability to create in vivo genomic medicines for tissues other than the liver has been impeded by difficulties in delivery. Using a high-throughput platform, we developed lipid nanoparticles that can effectively deliver mRNA and CRISPR–Cas9 gene editing tools to the lungs through intratracheal administration, expanding the potential clinical uses of gene editing and mRNA-based technologies.

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Fig. 1: LNP-enabled gene editing in mouse airway epithelium.

References

  1. Yin, H., Kauffman, K. J. & Anderson, D. G. Delivery technologies for genome editing. Nat. Rev. Drug Discov. 16, 387–399 (2017). This review article covers recent advances and challenges in nonviral and viral delivery methods.

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This is a summary of: Li, B. et al. Combinatorial design of nanoparticles for pulmonary mRNA delivery and genome editing. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01679-x (2023).

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Lipid nanoparticle-enabled gene editing in the lung via inhalation. Nat Biotechnol 41, 1394–1395 (2023). https://doi.org/10.1038/s41587-023-01689-9

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