Hyperfolder yellow fluorescent protein (hfYFP) and its variants are fluorescent proteins with high chemical and thermal stability. They resist aggregation, withstand diverse chemical challenges and show promise in expansion and electron microscopies. The chloride resistance and uncanny stability in guanidinium of hfYFP enable fluorescence-guided protein purification under denaturing conditions.
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This is a summary of: Campbell, B. C., Paez-Segala, M. G., Looger, L. L., Petsko, G. A. & Liu, C. F. Chemically stable fluorescent proteins for advanced microscopy. Nat. Methods https://doi.org/10.1038/s41592-022-01660-7 (2022).
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Robust fluorescent proteins for high-resolution microscopy and biochemical techniques. Nat Methods 19, 1534–1535 (2022). https://doi.org/10.1038/s41592-022-01661-6
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DOI: https://doi.org/10.1038/s41592-022-01661-6
