DISCOVER-seq+ enables the efficient detection of CRISPR–Cas off-target breaks in primary cells and tissues.
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Acknowledgements
We thank M. Bienko (Human Technopole and Karolinska Institutet) for providing insightful comments and suggestions. This work was supported by funding from the Swedish Research Council (grant. no. 2022-00721) to N.C. and by intramural funding from Human Technopole to C.J. and N.C.
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Jiménez, C., Crosetto, N. Discovering CRISPR–Cas off-target breaks. Nat Methods 20, 641–642 (2023). https://doi.org/10.1038/s41592-023-01847-6
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DOI: https://doi.org/10.1038/s41592-023-01847-6