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A 50 year history of technologies that drove discovery in eukaryotic transcription regulation

Nature Structural & Molecular Biology volume 26, pages 777–782 (2019)Cite this article

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Abstract

Transcription regulation is critical to organism development and homeostasis. Control of expression of the 20,000 genes in human cells requires many hundreds of proteins acting through sophisticated multistep mechanisms. In this Historical Perspective, I highlight the progress that has been made in elucidating eukaryotic transcriptional mechanisms through an array of disciplines and approaches, and how this concerted effort has been driven by the development of new technologies.

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Fig. 1: The transcription cycle consists of distinct steps that are targets of regulation.
Fig. 2: Dissecting eukaryotic transcriptional mechanisms in cells: the OPreO strategy.

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Acknowledgements

The author thanks members of his lab for valuable comments. The work is supported by US Public Health Service Award R01 GM025232 from the National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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  1. Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA

    John T. Lis

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Lis, J.T. A 50 year history of technologies that drove discovery in eukaryotic transcription regulation. Nat Struct Mol Biol 26, 777–782 (2019). https://doi.org/10.1038/s41594-019-0288-9

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