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DNA REPLICATION AND REPAIR

The partner-swapping sliding clamp loader exposed

Nature Structural & Molecular Biology volume 29, pages 283–286 (2022)Cite this article

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Eukaryotes possess several clamp loaders comprising four common subunits and a fifth subunit unique to each complex. The RFC-A–E clamp loader loads the PCNA clamp at 3′-recessed structures for DNA replication. However, swapping a single subunit, Rad24, for RFC-A yields a clamp loader that prefers the 911 clamp and 5′-recessed DNA. Three new studies reveal detailed views of the clamp loading reaction and provide insights into substrate preferences of each loader.

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Fig. 1: Sliding clamps and clamp loaders.
Fig. 2: The DNA replication and damage-response clamp loaders.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, City College of New York, New York, NY, USA

    David Jeruzalmi

  2. PhD Program in Biochemistry, The Graduate Center of the City University of New York, New York, NY, USA

    David Jeruzalmi

  3. PhD Program in Biology, The Graduate Center of the City University of New York, New York, NY, USA

    David Jeruzalmi

  4. PhD Program in Chemistry, The Graduate Center of the City University of New York, New York, NY, USA

    David Jeruzalmi

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  1. David Jeruzalmi
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Correspondence to David Jeruzalmi.

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The author declares no competing interests.

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Jeruzalmi, D. The partner-swapping sliding clamp loader exposed. Nat Struct Mol Biol 29, 283–286 (2022). https://doi.org/10.1038/s41594-022-00761-3

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