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DNA REPLICATION

Focus and persistence: how Pol IV unblocks stalled DNA synthesis

Nature Structural & Molecular Biology volume 29, pages 846–847 (2022)Cite this article

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The mechanisms by which translesion DNA polymerases mediate DNA repair are incompletely understood. A new study shows that Escherichia coli DNA polymerase IV is concentrated at the sites of arrested DNA synthesis by an interaction with SSB, the major single-stranded DNA-binding protein, specifically at stalled but not ongoing replication forks.

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Fig. 1: The two-step recruitment of Pol IV mediated by binding of SSB to ssDNA at sites of stalled DNA synthesis.

References

  1. Indiani, C., McInerney, P., Georgescu, R., Goodman, M. F. & O’Donnell, M. Mol. Cell 19, 805–815 (2005).

    Article  CAS  Google Scholar 

  2. Toste Rêgo, A., Holding, A. N., Kent, H. & Lamers, M. H. EMBO J. 32, 1334–1343 (2013).

    Article  Google Scholar 

  3. Jergic, S. et al. EMBO J. 32, 1322–1333 (2013).

    Article  CAS  Google Scholar 

  4. Zhao, G., Gleave, E. S. & Lamers, M. H. eLife 6, e32177 (2017).

    Article  Google Scholar 

  5. Kenyon, C. J. & Walker, G. C. Proc. Natl Acad. Sci. USA 77, 2819–2823 (1980).

    Article  CAS  Google Scholar 

  6. Kim, S. R., Matsui, K., Yamada, M., Gruz, P. & Nohmi, T. Mol. Genet. Genomics 266, 207–215 (2001).

    Article  CAS  Google Scholar 

  7. McHenry, C. & Kornberg, A. J. Biol. Chem. 252, 6478–6484 (1977).

    Article  CAS  Google Scholar 

  8. McKenzie, G. J., Magner, D. B., Lee, P. L. & Rosenberg, S. M. J. Bacteriol. 185, 3972–3977 (2003).

    Article  CAS  Google Scholar 

  9. Wolff, E., Kim, M., Hu, K., Yang, H. & Miller, J. J. Bacteriol. 186, 2900–2905 (2004).

    Article  CAS  Google Scholar 

  10. Kuban, W. et al. J. Bacteriol. 186, 4802–4807 (2004).

    Article  CAS  Google Scholar 

  11. Chang, S. et al. Proc. Natl Acad. Sci. USA 116, 25591–25601 (2019).

    Article  CAS  Google Scholar 

  12. Chang, S. et al. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-022-00827-2 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  13. Furukohri, A., Nishikawa, Y., Akiyama, M. T. & Maki, H. Nucleic Acids Res. 40, 6039–6048 (2012).

    Article  CAS  Google Scholar 

  14. Lohman, T. M. & Ferrari, M. E. Annu. Rev. Biochem. 63, 527–570 (1994).

    Article  CAS  Google Scholar 

  15. Sale, J. E., Lehmann, A. R. & Woodgate, R. Nat. Rev. Mol. Cell Biol. 13, 141–152 (2012).

    Article  CAS  Google Scholar 

  16. Gueranger, Q. et al. DNA Repair (Amst.) 7, 1551–1562 (2008).

    Article  CAS  Google Scholar 

  17. Spenkelink, L. M. et al. Nucleic Acids Res. 47, 4111–4123 (2019).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Work in the Sale laboratory is supported by a core grant to the LMB from the MRC (U105178808).

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  1. Division of Protein & Nucleic Acid Chemistry, MRC Laboratory of Molecular Biology, Cambridge, UK

    Julian E. Sale

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  1. Julian E. Sale
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Correspondence to Julian E. Sale.

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Sale, J.E. Focus and persistence: how Pol IV unblocks stalled DNA synthesis. Nat Struct Mol Biol 29, 846–847 (2022). https://doi.org/10.1038/s41594-022-00825-4

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