Browse Articles

  • Technical Report |

    Harada et al. develop a chromatin integration labelling (ChIL) method to map distributions of histone modifications and DNA-binding factors at low-input or even single-cell levels.

    • Akihito Harada
    • , Kazumitsu Maehara
    • , Tetsuya Handa
    • , Yasuhiro Arimura
    • , Jumpei Nogami
    • , Yoko Hayashi-Takanaka
    • , Katsuhiko Shirahige
    • , Hitoshi Kurumizaka
    • , Hiroshi Kimura
    •  & Yasuyuki Ohkawa
  • News & Views |

    RIPK1 plays a key role in several inflammatory and cell death signalling pathways. Understanding its regulation is pivotal for identifying diseases that might therapeutically benefit from RIPK1 inhibition. Recent studies now show that TBK1 and IKKε constitute a cell death checkpoint that restrains RIPK1 activation.

    • Klaus Heger
    •  & Vishva M. Dixit
  • News & Views |

    BAF is a heterogenous chromatin-remodelling complex, frequently mutated in cancer. A study now defines genome-wide localization patterns of three complexes, cBAF, PBAF and previously unknown ncBAF, and reveals the ncBAF complex as a specific vulnerability in synovial sarcoma and malignant rhabdoid tumours.

    • Divya Reddy
    •  & Jerry L. Workman
  • Editorial |

    Scientists are increasingly embracing social media in their professional lives. Here, we look at the different platforms available to researchers and how social media engagement can positively influence their day-to-day work and scientific communication.

  • Review Article |

    In this Review, Leidal et al. discuss the role and regulation of autophagy in aging. They cover how autophagy promotes longevity and restricts cellular damage, and discuss autophagy modulators for the potential treatment of age-related diseases.

    • Andrew M. Leidal
    • , Beth Levine
    •  & Jayanta Debnath
  • Review Article |

    Lawson et al. review recent advances in single-cell technologies and discuss in detail how they can be leveraged to understand tumour heterogeneity and metastasis.

    • Devon A. Lawson
    • , Kai Kessenbrock
    • , Ryan T. Davis
    • , Nicholas Pervolarakis
    •  & Zena Werb
  • Article |

    Avgustinova et al. report that targeting the H3K9 methyltransferase G9a in skin cancer does not affect single nucleotide variant profiles, but leads to increased tumour aggressiveness after a prolonged latency.

    • Alexandra Avgustinova
    • , Aikaterini Symeonidi
    • , Andrés Castellanos
    • , Uxue Urdiroz-Urricelqui
    • , Llorenç Solé-Boldo
    • , Mercè Martín
    • , Ivan Pérez-Rodríguez
    • , Neus Prats
    • , Ben Lehner
    • , Fran Supek
    •  & Salvador Aznar Benitah
  • News & Views |

    During mitosis, the kinetochore connects chromosomes to spindle microtubules and enables chromosome segregation. A genetic study in vertebrate cells demonstrates phosphorylation-regulated plasticity of kinetochore assembly and highlights the role of the centromere protein T in load-bearing kinetochore–microtubule attachment.

    • Yang Yang
    •  & Hongtao Yu
  • Letter |

    Xin et al. track stem cells in live mice to show that fate determination is a flexible process during homeostatic hair follicle growth, and progenitor cells can change differentiation outcomes as a consequence of dynamic relocation.

    • Tianchi Xin
    • , David Gonzalez
    • , Panteleimon Rompolas
    •  & Valentina Greco
  • Article |

    Lafont et al. uncover a checkpoint mediated by TBK1 and IKKε, which phosphorylate RIPK1 in the TNFR1-SC. TBK1 and IKKε recruitment depends on M1 ubiquitylation and NEMO to restrict TNF-induced cell death.

    • Elodie Lafont
    • , Peter Draber
    • , Eva Rieser
    • , Matthias Reichert
    • , Sebastian Kupka
    • , Diego de Miguel
    • , Helena Draberova
    • , Anne von Mässenhausen
    • , Amandeep Bhamra
    • , Stephen Henderson
    • , Katarzyna Wojdyla
    • , Avigayil Chalk
    • , Silvia Surinova
    • , Andreas Linkermann
    •  & Henning Walczak
  • Letter |

    di Ronza et al. identify CLN8 as a cargo receptor for lysosomal enzymes required for their endoplasmic-reticulum-to-Golgi transport, linking Batten disease caused by CLN8 mutations to defects in organelle biogenesis.

    • Alberto di Ronza
    • , Lakshya Bajaj
    • , Jaiprakash Sharma
    • , Deepthi Sanagasetti
    • , Parisa Lotfi
    • , Carolyn Joy Adamski
    • , John Collette
    • , Michela Palmieri
    • , Abdallah Amawi
    • , Lauren Popp
    • , Kevin Tommy Chang
    • , Maria Chiara Meschini
    • , Hon-Chiu Eastwood Leung
    • , Laura Segatori
    • , Alessandro Simonati
    • , Richard Norman Sifers
    • , Filippo Maria Santorelli
    •  & Marco Sardiello
  • Article |

    Michel et al. report unique localization of non-canonical BAF to CTCF sites and promoters, which confers synthetic lethality in canonical BAF-perturbed synovial sarcoma and malignant rhabdoid tumour cells.

    • Brittany C. Michel
    • , Andrew R. D’Avino
    • , Seth H. Cassel
    • , Nazar Mashtalir
    • , Zachary M. McKenzie
    • , Matthew J. McBride
    • , Alfredo M. Valencia
    • , Qianhe Zhou
    • , Michael Bocker
    • , Luis M. M. Soares
    • , Joshua Pan
    • , David I. Remillard
    • , Caleb A. Lareau
    • , Hayley J. Zullow
    • , Nora Fortoul
    • , Nathanael S. Gray
    • , James E. Bradner
    • , Ho Man Chan
    •  & Cigall Kadoch
  • News & Views |

    Newly synthesised lysosomal proteins are sorted from other cargo on the secretory pathway for delivery to endolysosomal compartments. A study now shows that the Batten disease protein, CLN8, acts as a recycling receptor to sort soluble lysosomal enzymes for export from the endoplasmic reticulum to the Golgi.

    • J. Paul Luzio
  • Article |

    Jung et al demonstrate that TMEM9 facilitates vacuolar-ATPase assembly to enhance vesicular acidification, thereby promoting activation of Wnt signalling and development of colorectal cancer.

    • Youn-Sang Jung
    • , Sohee Jun
    • , Moon Jong Kim
    • , Sung Ho Lee
    • , Han Na Suh
    • , Esther M. Lien
    • , Hae-Yun Jung
    • , Sunhye Lee
    • , Jie Zhang
    • , Jung-In Yang
    • , Hong Ji
    • , Ji Yuan Wu
    • , Wenqi Wang
    • , Rachel K. Miller
    • , Junjie Chen
    • , Pierre D. McCrea
    • , Scott Kopetz
    •  & Jae-Il Park
  • Review Article |

    Goddard et al review recent advances in our understanding of dormant tumour cells, highlight their cross-talk with immune responses, and elaborate therapeutic implications to treat metastatic malignancies.

    • Erica T. Goddard
    • , Ivana Bozic
    • , Stanley R. Riddell
    •  & Cyrus M. Ghajar
  • News & Views |

    The cancer-suppressive mechanisms underlying a tissue’s response to spontaneous oncogenic mutations during homeostasis are largely unknown. A study now explores how clonal expansion of epidermal stem cells with specific oncogenic mutations might be restricted by their elimination through enforced differentiation.

    • Paola Kuri
    •  & Panteleimon Rompolas
  • Article |

    Jung et al. demonstrate that loss of CRAD impairs F-actin polymerization and disrupts the cadherin–catenin–actin complex, thereby hyperactivating Wnt signalling and promoting mucinous intestinal tumorigenesis.

    • Youn-Sang Jung
    • , Wenqi Wang
    • , Sohee Jun
    • , Jie Zhang
    • , Mrinal Srivastava
    • , Moon Jong Kim
    • , Esther M. Lien
    • , Joan Shang
    • , Junjie Chen
    • , Pierre D. McCrea
    • , Songlin Zhang
    •  & Jae-Il Park
  • Article |

    Lock et al. identify reticular adhesion complexes that maintain cell–extracellular-matrix attachments during cell division. Reticular adhesions transmit spatial memory between cell generations, mediated by αvβ5 integrin and PtdIns(4,5)P2.

    • John G. Lock
    • , Matthew C. Jones
    • , Janet A. Askari
    • , Xiaowei Gong
    • , Anna Oddone
    • , Helene Olofsson
    • , Sara Göransson
    • , Melike Lakadamyali
    • , Martin J. Humphries
    •  & Staffan Strömblad
  • News & Views |

    Classical actin-dependent, integrin-mediated cell–matrix adhesions disassemble before mitotic rounding. Yet, to transmit positional information and facilitate daughter-cell separation, dividing cells maintain connections to the matrix. A previously unidentified class of actin-independent integrin adhesions may fulfil this task.

    • Ronen Zaidel-Bar
  • News & Views |

    β-catenin regulates cell–cell adhesion and maintains stemness through Wnt signalling, but how these functions are mechanistically related is not fully understood. A study now identifies CRAD as the mechanistic link, providing insight into how dysregulation of epithelial adhesion contributes to Wnt-driven tumorigenesis.

    • George Eng
    • , Jonathan Braverman
    •  & Ömer H. Yilmaz
  • Article |

    Cigliola et al. show that β-cell loss activates insulin production in a small number of α-cells and that insulin and Hedgehog signalling actively maintain and enforce the α-cell fate.

    • Valentina Cigliola
    • , Luiza Ghila
    • , Fabrizio Thorel
    • , Léon van Gurp
    • , Delphine Baronnier
    • , Daniel Oropeza
    • , Simone Gupta
    • , Takeshi Miyatsuka
    • , Hideaki Kaneto
    • , Mark A. Magnuson
    • , Anna B. Osipovich
    • , Maike Sander
    • , Christopher E. V. Wright
    • , Melissa K. Thomas
    • , Kenichiro Furuyama
    • , Simona Chera
    •  & Pedro L. Herrera
  • Article |

    Using time-lapse microscopy and transcriptome analysis of the post-implantation mouse embryo, Christodoulou et al. show that cavity fusion occurs through the formation and polarized resolution of multiple, multicellular three-dimensional rosettes.

    • Neophytos Christodoulou
    • , Christos Kyprianou
    • , Antonia Weberling
    • , Ran Wang
    • , Guizhong Cui
    • , Guangdun Peng
    • , Naihe Jing
    •  & Magdalena Zernicka-Goetz
  • News & Views |

    Functional genetic screening of mice and other mammals is exceedingly challenging. A CRISPR-based mutagenesis screen in mice has successfully revealed amino acids vital for protein function of the DND1 gene, missense mutations of which lead to defects in primordial germ cell development.

    • Yevgeniy V. Serebrenik
    •  & Ophir Shalem
  • Technical Report |

    Li and colleagues develop a CRISPR–Cas9-based screen strategy that combines base editing and haploid embryonic stem cell technologies to identify amino acids critical for protein function in mice.

    • Qing Li
    • , Yanjing Li
    • , Suming Yang
    • , Shuo Huang
    • , Meng Yan
    • , Yifu Ding
    • , Wei Tang
    • , Xiwen Lou
    • , Qi Yin
    • , Zhanfei Sun
    • , Lei Lu
    • , Huijuan Shi
    • , Hongyan Wang
    • , Yong Chen
    •  & Jinsong Li
  • News & Views |

    The intestinal crypt has become the prototype compartment to investigate adult stem cell biology, and the list of identified intestinal stem cell (ISC) markers is already extensive. A comprehensive study now uncovers an additional layer in ISC regulation by introducing long noncoding RNA lncGata6 to the stem cell repertoire.

    • Pantelis Hatzis
    •  & Hugo J. G. Snippert
  • News & Views |

    Intra-tumour heterogeneity manifests both at the level of mutational burden, and at a functional level within genetically homogenous populations. A new modelling approach suggests stemness within colorectal tumours is defined by microenvironmental cues secreted from cancer-associated fibroblasts rather than cell-intrinsic properties.

    • D. J. Flanagan
    • , M. C. Hodder
    •  & O. J. Sansom
  • Editorial |

    We are often asked about various aspects of the editor’s job, and most frequently about the editorial process after submission. Here, we outline what happens after a manuscript is submitted to the journal and clarify some misconceptions about the editorial process.

  • Article |

    Basnet et al. discover that in vitro SSNA1 forms fibrils that attach along protofilaments and guide these to grow away from microtubules, forming templates for branched microtubules. SSNA1 mutations that perturb this process lead to defective axon branching in primary neurons.

    • Nirakar Basnet
    • , Hana Nedozralova
    • , Alvaro H. Crevenna
    • , Satish Bodakuntla
    • , Thomas Schlichthaerle
    • , Michael Taschner
    • , Giovanni Cardone
    • , Carsten Janke
    • , Ralf Jungmann
    • , Maria M. Magiera
    • , Christian Biertümpfel
    •  & Naoko Mizuno
  • News & Views |

    Paraspeckles are nuclear bodies built on the long noncoding RNA, NEAT1, that regulate cellular homeostasis, but how they sense and help under stress is unclear. A study now shows mitochondrial stress modulates paraspeckles by altering NEAT1 expression with a feedback loop that influences mitochondrial homeostasis.

    • Archa H. Fox
  • Article |

    Fort et al. identify CYRI as a conserved negative modulator of Scar–WAVE-induced lamellipodia by interacting directly with active Rac1, thereby conferring pseudopod plasticity and dynamics during motility.

    • Loic Fort
    • , José Miguel Batista
    • , Peter A. Thomason
    • , Heather J. Spence
    • , Jamie A. Whitelaw
    • , Luke Tweedy
    • , Jennifer Greaves
    • , Kirsty J. Martin
    • , Kurt I. Anderson
    • , Peter Brown
    • , Sergio Lilla
    • , Matthew P. Neilson
    • , Petra Tafelmeyer
    • , Sara Zanivan
    • , Shehab Ismail
    • , David M. Bryant
    • , Nicholas C. O. Tomkinson
    • , Luke H. Chamberlain
    • , Grant S. Mastick
    • , Robert H. Insall
    •  & Laura M. Machesky
  • Technical Report |

    Mehta et al. create single-fluorophore kinase activity sensors for PKA, PKC and ERK in different colours, and demonstrate that they enable multiplex imaging of a combination of signalling activities in cell lines and primary rat neurons.

    • Sohum Mehta
    • , Yong Zhang
    • , Richard H. Roth
    • , Jin-fan Zhang
    • , Albert Mo
    • , Brian Tenner
    • , Richard L. Huganir
    •  & Jin Zhang
  • Review Article |

    In this Review, Doherty and Baehrecke discuss the multiple roles of autophagy during cell survival and cell death. They cover the interplay between autophagy, apoptosis and necrosis, as well as engulfment and inflammation.

    • Johnna Doherty
    •  & Eric H. Baehrecke
  • News & Views |

    Ferroptosis is a regulated non-apoptotic form of cell death and its functional role in tumorigenesis remains elusive. A study now shows that the tumour suppressor BAP1 enhances ferroptosis by modulating expression of the cystine transporter SLC7A11, leading to improved control of tumour growth.

    • Michael P. Murphy
  • Article |

    Zhu et al. show that the long noncoding RNA lncGata6 enhances Wnt signalling in intestinal stem cells by recruiting the NURF remodelling complex onto the Ehf promoter and initiating its transcription, which triggers expression of Lgr4/5.

    • Pingping Zhu
    • , Jiayi Wu
    • , Yanying Wang
    • , Xiaoxiao Zhu
    • , Tiankun Lu
    • , Benyu Liu
    • , Luyun He
    • , Buqing Ye
    • , Shuo Wang
    • , Shu Meng
    • , Dongdong Fan
    • , Jing Wang
    • , Liuliu Yang
    • , Xiwen Qin
    • , Ying Du
    • , Chong Li
    • , Lei He
    • , Weizheng Ren
    • , Xin Wu
    • , Yong Tian
    •  & Zusen Fan
  • Letter |

    Using super-resolution microscopy, tracking of single particle trajectories of endoplasmic reticulum (ER) luminal proteins traversing tubular ER, and measuring ER dynamics, Holcman et al. show that ER content is propelled by active luminal flow.

    • David Holcman
    • , Pierre Parutto
    • , Joseph E. Chambers
    • , Marcus Fantham
    • , Laurence J. Young
    • , Stefan J. Marciniak
    • , Clemens F. Kaminski
    • , David Ron
    •  & Edward Avezov
  • Article |

    Barnes et al. report a dynamic and reciprocal crosstalk between tissue tension and glycocalyx bulkiness that promotes a mesenchymal, stem-like phenotype in GBM.

    • J. Matthew Barnes
    • , Shelly Kaushik
    • , Russell O. Bainer
    • , Jason K. Sa
    • , Elliot C. Woods
    • , FuiBoon Kai
    • , Laralynne Przybyla
    • , Mijeong Lee
    • , Hye Won Lee
    • , Jason C. Tung
    • , Ori Maller
    • , Alexander S. Barrett
    • , Kan V. Lu
    • , Jonathon N. Lakins
    • , Kirk C. Hansen
    • , Kirsten Obernier
    • , Arturo Alvarez-Buylla
    • , Gabriele Bergers
    • , Joanna J. Phillips
    • , Do-Hyun Nam
    • , Carolyn R. Bertozzi
    •  & Valerie M. Weaver
  • Letter |

    Studying early Drosophila wing epithelium development, Singh et al. find that individual cells are mechanically autonomous, and that their elongation along the proximal–distal axis is driven by polarized microtubule-mediated pushing forces.

    • Amrita Singh
    • , Tanumoy Saha
    • , Isabell Begemann
    • , Andrea Ricker
    • , Harald Nüsse
    • , Oliver Thorn-Seshold
    • , Jürgen Klingauf
    • , Milos Galic
    •  & Maja Matis
  • Article |

    Zhang et al. show that BAP1 suppresses SLC7A11 expression and cystine uptake, thereby promoting ferroptosis and inhibiting tumour growth.

    • Yilei Zhang
    • , Jiejun Shi
    • , Xiaoguang Liu
    • , Li Feng
    • , Zihua Gong
    • , Pranavi Koppula
    • , Kapil Sirohi
    • , Xu Li
    • , Yongkun Wei
    • , Hyemin Lee
    • , Li Zhuang
    • , Gang Chen
    • , Zhen-Dong Xiao
    • , Mien-Chie Hung
    • , Junjie Chen
    • , Peng Huang
    • , Wei Li
    •  & Boyi Gan
  • Correction |

    • Rishi Raj Chhipa
    • , Qiang Fan
    • , Jane Anderson
    • , Ranjithmenon Muraleedharan
    • , Yan Huang
    • , Georgianne Ciraolo
    • , Xiaoting Chen
    • , Ronald Waclaw
    • , Lionel M. Chow
    • , Zaza Khuchua
    • , Matthew Kofron
    • , Matthew T. Weirauch
    • , Ady Kendler
    • , Christopher McPherson
    • , Nancy Ratner
    • , Ichiro Nakano
    • , Nupur Dasgupta
    • , Kakajan Komurov
    •  & Biplab Dasgupta