Nature Methods - AOP - nature.com science feeds Nature Methods is a science methodology journal publishing laboratory techniques and methods papers in the life sciences and areas of chemistry relevant to the life sciences. http://www.nature.com/nmeth/current_issue/ Nature Publishing Group en © Nature Publishing Group Nature Methods 1548-7091 1548-7105 © Nature Publishing Group permissions@nature.com Nature Methods http://www.nature.com/includes/rj_globnavimages/nmeth_logo.gif http://www.nature.com/nmeth/ Cell stimulation with optically manipulated microsources http://feeds.nature.com/~r/nmeth/rss/aop/~3/MIJNB5XVPtY/nmeth.1400 Microsources positioned with holographic optical tweezers can establish a highly localized, three-dimensional chemical gradient that allows the manipulation of polarization and migration in single cells. Cell stimulation with optically manipulated microsources

Nature Methods. doi:10.1038/nmeth.1400

Authors: Holger Kress, Jin-Gyu Park, Cecile O Mejean, Jason D Forster, Jason Park, Spencer S Walse, Yong Zhang, Dianqing Wu, Orion D Weiner, Tarek M Fahmy & Eric R Dufresne

]]> Cell stimulation with optically manipulated microsources Holger Kress Jin-Gyu Park Cecile O Mejean Jason D Forster Jason Park Spencer S Walse Yong Zhang Dianqing Wu Orion D Weiner Tarek M Fahmy Eric R Dufresne doi:10.1038/nmeth.1400 Nature Methods 2009-11-15 Nature Methods 2009-11-15 10.1038/nmeth.1400 http://dx.doi.org/10.1038/nmeth.1400 Article http://dx.doi.org/10.1038/nmeth.1400 Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms http://feeds.nature.com/~r/nmeth/rss/aop/~3/-C3PqXqmJ9U/nmeth.1402 Tissue-specific expression of microRNA sponges allows precise regulation of microRNA activity in living flies. The authors investigate the role of miR-8 in the formation of neuromuscular junctions in detail. Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms

Nature Methods. doi:10.1038/nmeth.1402

Authors: Carlos M Loya, Cecilia S Lu, David Van Vactor & Tudor A Fulga

]]> Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms Carlos M Loya Cecilia S Lu David Van Vactor Tudor A Fulga doi:10.1038/nmeth.1402 Nature Methods 2009-11-15 Nature Methods 2009-11-15 10.1038/nmeth.1402 http://dx.doi.org/10.1038/nmeth.1402 Article http://dx.doi.org/10.1038/nmeth.1402 An auxin-based degron system for the rapid depletion of proteins in nonplant cells http://feeds.nature.com/~r/nmeth/rss/aop/~3/ua8lLmIFch0/nmeth.1401 A degradation pathway found in plants, dependent on the hormone auxin, can be transplanted and harnessed to induce rapid and reversible target protein degradation in both yeast and animal cells. An auxin-based degron system for the rapid depletion of proteins in nonplant cells

Nature Methods. doi:10.1038/nmeth.1401

Authors: Kohei Nishimura, Tatsuo Fukagawa, Haruhiko Takisawa, Tatsuo Kakimoto & Masato Kanemaki

]]> An auxin-based degron system for the rapid depletion of proteins in nonplant cells Kohei Nishimura Tatsuo Fukagawa Haruhiko Takisawa Tatsuo Kakimoto Masato Kanemaki doi:10.1038/nmeth.1401 Nature Methods 2009-11-15 Nature Methods 2009-11-15 10.1038/nmeth.1401 http://dx.doi.org/10.1038/nmeth.1401 Article http://dx.doi.org/10.1038/nmeth.1401 A genetically encoded reporter of synaptic activity in vivo http://feeds.nature.com/~r/nmeth/rss/aop/~3/sPE4pQ63BGE/nmeth.1399 Fusion of the genetically-encoded calcium indicator GCaMP2 to synaptophysin localizes the sensor to neuron presynaptic terminals and conveys linear responsiveness over a wider range of spike frequencies. The sensor allowed measurement of synaptic activity caused by spiking as well as graded voltage signals during in vivo imaging in zebrafish. A genetically encoded reporter of synaptic activity in vivo

Nature Methods. doi:10.1038/nmeth.1399

Authors: Elena Dreosti, Benjamin Odermatt, Mario M Dorostkar & Leon Lagnado

]]> A genetically encoded reporter of synaptic activity in vivo Elena Dreosti Benjamin Odermatt Mario M Dorostkar Leon Lagnado doi:10.1038/nmeth.1399 Nature Methods 2009-11-08 Nature Methods 2009-11-08 10.1038/nmeth.1399 http://dx.doi.org/10.1038/nmeth.1399 Article http://dx.doi.org/10.1038/nmeth.1399 Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos http://feeds.nature.com/~r/nmeth/rss/aop/~3/BFondmOeuaI/nmeth.1396 Methods for automated fluorescence imaging allow high-throughput examination of reporter expression patterns in zebrafish embryos. They are applied to mapping promoter-enhancer interactions in this organism. Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos

Nature Methods. doi:10.1038/nmeth.1396

Authors: Jochen Gehrig, Markus Reischl, Éva Kalmár, Marco Ferg, Yavor Hadzhiev, Andreas Zaucker, Chengyi Song, Simone Schindler, Urban Liebel & Ferenc Müller

]]> Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos Jochen Gehrig Markus Reischl Éva Kalmár Marco Ferg Yavor Hadzhiev Andreas Zaucker Chengyi Song Simone Schindler Urban Liebel Ferenc Müller doi:10.1038/nmeth.1396 Nature Methods 2009-11-08 Nature Methods 2009-11-08 10.1038/nmeth.1396 http://dx.doi.org/10.1038/nmeth.1396 Article http://dx.doi.org/10.1038/nmeth.1396 Optical interrogation of neural circuits in Caenorhabditis elegans http://feeds.nature.com/~r/nmeth/rss/aop/~3/EkWt5xM_JdU/nmeth.1397 Neuronal stimulation with channelrhodopsin-2 is combined with calcium fluorescence imaging to study neural connections in intact Caenorhabditis elegans. Optical interrogation of neural circuits in Caenorhabditis elegans

Nature Methods. doi:10.1038/nmeth.1397

Authors: Zengcai V Guo, Anne C Hart & Sharad Ramanathan

]]> Optical interrogation of neural circuits in Caenorhabditis elegans Zengcai V Guo Anne C Hart Sharad Ramanathan doi:10.1038/nmeth.1397 Nature Methods 2009-11-08 Nature Methods 2009-11-08 10.1038/nmeth.1397 http://dx.doi.org/10.1038/nmeth.1397 Article http://dx.doi.org/10.1038/nmeth.1397 Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators http://feeds.nature.com/~r/nmeth/rss/aop/~3/ENrhj3PwEm0/nmeth.1398 An improved version of the GCaMP genetically encoded calcium indicator, called GCaMP3, has higher calcium affinity and increased baseline fluorescence, dynamic range and stability. GCaMP3 performs better than existing genetically encoded calcium indicators in several assays and organisms, including in vivo imaging of neuronal signaling in worms, flies and mice. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

Nature Methods. doi:10.1038/nmeth.1398

Authors: Lin Tian, S Andrew Hires, Tianyi Mao, Daniel Huber, M Eugenia Chiappe, Sreekanth H Chalasani, Leopoldo Petreanu, Jasper Akerboom, Sean A McKinney, Eric R Schreiter, Cornelia I Bargmann, Vivek Jayaraman, Karel Svoboda & Loren L Looger

]]> Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators Lin Tian S Andrew Hires Tianyi Mao Daniel Huber M Eugenia Chiappe Sreekanth H Chalasani Leopoldo Petreanu Jasper Akerboom Sean A McKinney Eric R Schreiter Cornelia I Bargmann Vivek Jayaraman Karel Svoboda Loren L Looger doi:10.1038/nmeth.1398 Nature Methods 2009-11-08 Nature Methods 2009-11-08 10.1038/nmeth.1398 http://dx.doi.org/10.1038/nmeth.1398 Article http://dx.doi.org/10.1038/nmeth.1398 High-speed nanoscopic tracking of the position and orientation of a single virus http://feeds.nature.com/~r/nmeth/rss/aop/~3/XhQMJdVtKdI/nmeth.1395 A combination of scattering interferometry and single-molecule fluorescence microscopy allows visualization of both the position and orientation of single Simian virus 40 particles on lipid bilayers and provides evidence of viral interaction with receptors in membrane nanodomains. High-speed nanoscopic tracking of the position and orientation of a single virus

Nature Methods. doi:10.1038/nmeth.1395

Authors: Philipp Kukura, Helge Ewers, Christian Müller, Alois Renn, Ari Helenius & Vahid Sandoghdar

]]> High-speed nanoscopic tracking of the position and orientation of a single virus Philipp Kukura Helge Ewers Christian Müller Alois Renn Ari Helenius Vahid Sandoghdar doi:10.1038/nmeth.1395 Nature Methods 2009-11-01 Nature Methods 2009-11-01 10.1038/nmeth.1395 http://dx.doi.org/10.1038/nmeth.1395 Article http://dx.doi.org/10.1038/nmeth.1395