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<title>Nature Methods</title>
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<title>Enlightened neuroscience</title>
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<p>
<b>Enlightened neuroscience</b>
</p>
<p>Nature Methods 6, 857 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-857">doi:10.1038/nmeth1209-857</a>
</p>
<p>Neuroscience methods are undergoing a dramatic change owing to improvements in optical probes, but standardized evaluation procedures would aid probe development and uptake.</p>
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<title>Cell culture medium affects GFP photostability: a solution</title>
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<p>
<b>Cell culture medium affects GFP photostability: a solution</b>
</p>
<p>Nature Methods 6, 859 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-859">doi:10.1038/nmeth1209-859</a>
</p>
<p>Authors: Alexey M Bogdanov, Ekaterina A Bogdanova, Dmitriy M Chudakov, Tatiana V Gorodnicheva, Sergey Lukyanov &amp; Konstantin A Lukyanov</p>
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<dc:creator>Ekaterina A Bogdanova</dc:creator>
<dc:creator>Dmitriy M Chudakov</dc:creator>
<dc:creator>Tatiana V Gorodnicheva</dc:creator>
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<dc:creator>Konstantin A Lukyanov</dc:creator>
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<title>Recurated protein interaction datasets</title>
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<description />
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<p>
<b>Recurated protein interaction datasets</b>
</p>
<p>Nature Methods 6, 860 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-860">doi:10.1038/nmeth1209-860</a>
</p>
<p>Authors: Lukasz Salwinski, Luana Licata, Andrew Winter, David Thorneycroft, Jyoti Khadake, Arnaud Ceol, Andrew Chatr Aryamontri, Rose Oughtred, Michael Livstone, Lorrie Boucher, David Botstein, Kara Dolinski, Tanya Berardini, Eva Huala, Mike Tyers, David Eisenberg, Gianni Cesareni &amp; Henning Hermjakob</p>
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<dc:title>Recurated protein interaction datasets</dc:title>
<dc:creator>Lukasz Salwinski</dc:creator>
<dc:creator>Luana Licata</dc:creator>
<dc:creator>Andrew Winter</dc:creator>
<dc:creator>David Thorneycroft</dc:creator>
<dc:creator>Jyoti Khadake</dc:creator>
<dc:creator>Arnaud Ceol</dc:creator>
<dc:creator>Andrew Chatr Aryamontri</dc:creator>
<dc:creator>Rose Oughtred</dc:creator>
<dc:creator>Michael Livstone</dc:creator>
<dc:creator>Lorrie Boucher</dc:creator>
<dc:creator>David Botstein</dc:creator>
<dc:creator>Kara Dolinski</dc:creator>
<dc:creator>Tanya Berardini</dc:creator>
<dc:creator>Eva Huala</dc:creator>
<dc:creator>Mike Tyers</dc:creator>
<dc:creator>David Eisenberg</dc:creator>
<dc:creator>Gianni Cesareni</dc:creator>
<dc:creator>Henning Hermjakob</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-860</dc:identifier>
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<title>When ChIA PETs meet Hi-C</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/G-2KeYXVXWM/nmeth1209-863</link>
<description>Two methods enable the drawing of genome-wide chromatin interaction maps: one looks at protein-independent folding principles, the other at protein-mediated functional interactions.</description>
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<p>
<b>When ChIA PETs meet Hi-C</b>
</p>
<p>Nature Methods 6, 863 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-863">doi:10.1038/nmeth1209-863</a>
</p>
<p>Author: Nicole Rusk</p>
<p>Two methods enable the drawing of genome-wide chromatin interaction maps: one looks at protein-independent folding principles, the other at protein-mediated functional interactions.</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/G-2KeYXVXWM" height="1" width="1"/>]]></content:encoded>
<dc:title>When ChIA PETs meet Hi-C</dc:title>
<dc:creator>Nicole Rusk</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-863</dc:identifier>
<dc:source>Nature Methods 6, 863 (2009)</dc:source>
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<title>Many routes to pluripotency</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/IGRUISvSNRU/nmeth1209-864a</link>
<description>Researchers observe that cells of the post-implantation mouse epiblast can revert to an embryonic stem cell–like state without the addition of exogenous genes.</description>
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<p>
<b>Many routes to pluripotency</b>
</p>
<p>Nature Methods 6, 864 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-864a">doi:10.1038/nmeth1209-864a</a>
</p>
<p>Author: Natalie de Souza</p>
<p>Researchers observe that cells of the post-implantation mouse epiblast can revert to an embryonic stem cell&#8211;like state without the addition of exogenous genes.</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/IGRUISvSNRU" height="1" width="1"/>]]></content:encoded>
<dc:title>Many routes to pluripotency</dc:title>
<dc:creator>Natalie de Souza</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-864a</dc:identifier>
<dc:source>Nature Methods 6, 864 (2009)</dc:source>
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<prism:startingPage>864</prism:startingPage>
<prism:endingPage>865</prism:endingPage>
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<title>Looking for a reaction</title>
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<description>New chemical microarrays capture a comprehensive snapshot of the various enzymatic activities contained within a biological sample.</description>
<content:encoded><![CDATA[

<p>
<b>Looking for a reaction</b>
</p>
<p>Nature Methods 6, 864 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-864b">doi:10.1038/nmeth1209-864b</a>
</p>
<p>Author: Michael Eisenstein</p>
<p>New chemical microarrays capture a comprehensive snapshot of the various enzymatic activities contained within a biological sample.</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/Uf7Ehl7rnFA" height="1" width="1"/>]]></content:encoded>
<dc:title>Looking for a reaction</dc:title>
<dc:creator>Michael Eisenstein</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-864b</dc:identifier>
<dc:source>Nature Methods 6, 864 (2009)</dc:source>
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<prism:startingPage>864</prism:startingPage>
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<title>News in brief</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/Yx7VVhtkv_E/nmeth1209-865</link>
<description />
<content:encoded><![CDATA[

<p>
<b>News in brief</b>
</p>
<p>Nature Methods 6, 865 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-865">doi:10.1038/nmeth1209-865</a>
</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/Yx7VVhtkv_E" height="1" width="1"/>]]></content:encoded>
<dc:title>News in brief</dc:title>
<dc:identifier>doi:10.1038/nmeth1209-865</dc:identifier>
<dc:source>Nature Methods 6, 865 (2009)</dc:source>
<prism:publicationName>Nature Methods</prism:publicationName>
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<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Research Highlights</prism:section>
<prism:startingPage>865</prism:startingPage>
<prism:endingPage>865</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth1209-865</feedburner:origLink></item>
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<title>Seeing in the dark</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/Nd97gFwDiQE/nmeth1209-866</link>
<description>An imaging platform based on stimulated emission helps researchers to lead nonfluorescent chromophores out of the shadows.</description>
<content:encoded><![CDATA[

<p>
<b>Seeing in the dark</b>
</p>
<p>Nature Methods 6, 866 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-866">doi:10.1038/nmeth1209-866</a>
</p>
<p>Author: Michael Eisenstein</p>
<p>An imaging platform based on stimulated emission helps researchers to lead nonfluorescent chromophores out of the shadows.</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/Nd97gFwDiQE" height="1" width="1"/>]]></content:encoded>
<dc:title>Seeing in the dark</dc:title>
<dc:creator>Michael Eisenstein</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-866</dc:identifier>
<dc:source>Nature Methods 6, 866 (2009)</dc:source>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:doi>10.1038/nmeth1209-866</prism:doi>
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<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Research Highlights</prism:section>
<prism:startingPage>866</prism:startingPage>
<prism:endingPage>866</prism:endingPage>
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<title>Scattering gold</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/7CEOI_Q0UmA/nmeth1209-868</link>
<description>Gold nanoparticles are used to monitor caspase activity at the single-molecule level in living cells.</description>
<content:encoded><![CDATA[

<p>
<b>Scattering gold</b>
</p>
<p>Nature Methods 6, 868 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-868">doi:10.1038/nmeth1209-868</a>
</p>
<p>Author: Natalie de Souza</p>
<p>Gold nanoparticles are used to monitor caspase activity at the single-molecule level in living cells.</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/7CEOI_Q0UmA" height="1" width="1"/>]]></content:encoded>
<dc:title>Scattering gold</dc:title>
<dc:creator>Natalie de Souza</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-868</dc:identifier>
<dc:source>Nature Methods 6, 868 (2009)</dc:source>
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<prism:volume>6</prism:volume>
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<prism:startingPage>868</prism:startingPage>
<prism:endingPage>868</prism:endingPage>
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<title>Lighting up neural networks using a new generation of genetically encoded calcium sensors</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/-MOSUTJOd4Q/nmeth1209-871</link>
<description />
<content:encoded><![CDATA[

<p>
<b>Lighting up neural networks using a new generation of genetically encoded calcium sensors</b>
</p>
<p>Nature Methods 6, 871 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-871">doi:10.1038/nmeth1209-871</a>
</p>
<p>Authors: Christian D Wilms &amp; Michael H&#228;usser</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/-MOSUTJOd4Q" height="1" width="1"/>]]></content:encoded>
<dc:title>Lighting up neural networks using a new generation of genetically encoded calcium sensors</dc:title>
<dc:creator>Christian D Wilms</dc:creator>
<dc:creator>Michael Häusser</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-871</dc:identifier>
<dc:source>Nature Methods 6, 871 (2009)</dc:source>
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<prism:volume>6</prism:volume>
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<prism:section>News and Views</prism:section>
<prism:startingPage>871</prism:startingPage>
<prism:endingPage>872</prism:endingPage>
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<title>Use of microRNA sponges to explore tissue-specific microRNA functions in vivo</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/_ONPm55Klvg/nmeth1209-873</link>
<description />
<content:encoded><![CDATA[

<p>
<b>Use of microRNA sponges to explore tissue-specific microRNA functions in vivo</b>
</p>
<p>Nature Methods 6, 873 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-873">doi:10.1038/nmeth1209-873</a>
</p>
<p>Author: Stephen M Cohen</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/_ONPm55Klvg" height="1" width="1"/>]]></content:encoded>
<dc:title>Use of microRNA sponges to explore tissue-specific microRNA functions in vivo</dc:title>
<dc:creator>Stephen M Cohen</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-873</dc:identifier>
<dc:source>Nature Methods 6, 873 (2009)</dc:source>
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<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>News and Views</prism:section>
<prism:startingPage>873</prism:startingPage>
<prism:endingPage>874</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth1209-873</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth.1398">
<title>Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/ENrhj3PwEm0/nmeth.1398</link>
<description>An improved version of the GCaMP genetically encoded calcium indicator, called GCaMP3, has higher calcium affinity and increased baseline fluorescence, dynamic range and stability. GCaMP3 performs better than existing genetically encoded calcium indicators in several assays and organisms, including in vivo imaging of neuronal signaling in worms, flies and mice.</description>
<content:encoded><![CDATA[

<p>
<b>Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators</b>
</p>
<p>Nature Methods 6, 875 (2009). <a href="http://dx.doi.org/10.1038/nmeth.1398">doi:10.1038/nmeth.1398</a>
</p>
<p>Authors: Lin Tian, S Andrew Hires, Tianyi Mao, Daniel Huber, M Eugenia Chiappe, Sreekanth H Chalasani, Leopoldo Petreanu, Jasper Akerboom, Sean A McKinney, Eric R Schreiter, Cornelia I Bargmann, Vivek Jayaraman, Karel Svoboda &amp; Loren L Looger</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/ENrhj3PwEm0" height="1" width="1"/>]]></content:encoded>
<dc:title>Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators</dc:title>
<dc:creator>Lin Tian</dc:creator>
<dc:creator>S Andrew Hires</dc:creator>
<dc:creator>Tianyi Mao</dc:creator>
<dc:creator>Daniel Huber</dc:creator>
<dc:creator>M Eugenia Chiappe</dc:creator>
<dc:creator>Sreekanth H Chalasani</dc:creator>
<dc:creator>Leopoldo Petreanu</dc:creator>
<dc:creator>Jasper Akerboom</dc:creator>
<dc:creator>Sean A McKinney</dc:creator>
<dc:creator>Eric R Schreiter</dc:creator>
<dc:creator>Cornelia I Bargmann</dc:creator>
<dc:creator>Vivek Jayaraman</dc:creator>
<dc:creator>Karel Svoboda</dc:creator>
<dc:creator>Loren L Looger</dc:creator>
<dc:identifier>doi:10.1038/nmeth.1398</dc:identifier>
<dc:source>Nature Methods 6, 875 (2009)</dc:source>
<dc:date>2009-11-08</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-08</prism:publicationDate>
<prism:doi>10.1038/nmeth.1398</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth.1398</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Article</prism:section>
<prism:startingPage>875</prism:startingPage>
<prism:endingPage>881</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth.1398</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth.1399">
<title>A genetically encoded reporter of synaptic activity in vivo</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/sPE4pQ63BGE/nmeth.1399</link>
<description>Fusion of the genetically-encoded calcium indicator GCaMP2 to synaptophysin localizes the sensor to neuron presynaptic terminals and conveys linear responsiveness over a wider range of spike frequencies. The sensor allowed measurement of synaptic activity caused by spiking as well as graded voltage signals during in vivo imaging in zebrafish.</description>
<content:encoded><![CDATA[

<p>
<b>A genetically encoded reporter of synaptic activity in vivo</b>
</p>
<p>Nature Methods 6, 883 (2009). <a href="http://dx.doi.org/10.1038/nmeth.1399">doi:10.1038/nmeth.1399</a>
</p>
<p>Authors: Elena Dreosti, Benjamin Odermatt, Mario M Dorostkar &amp; Leon Lagnado</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/sPE4pQ63BGE" height="1" width="1"/>]]></content:encoded>
<dc:title>A genetically encoded reporter of synaptic activity in vivo</dc:title>
<dc:creator>Elena Dreosti</dc:creator>
<dc:creator>Benjamin Odermatt</dc:creator>
<dc:creator>Mario M Dorostkar</dc:creator>
<dc:creator>Leon Lagnado</dc:creator>
<dc:identifier>doi:10.1038/nmeth.1399</dc:identifier>
<dc:source>Nature Methods 6, 883 (2009)</dc:source>
<dc:date>2009-11-08</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-08</prism:publicationDate>
<prism:doi>10.1038/nmeth.1399</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth.1399</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Article</prism:section>
<prism:startingPage>883</prism:startingPage>
<prism:endingPage>889</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth.1399</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth.1397">
<title>Optical interrogation of neural circuits in Caenorhabditis elegans</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/EkWt5xM_JdU/nmeth.1397</link>
<description>Neuronal stimulation with channelrhodopsin-2 is combined with calcium fluorescence imaging to study neural connections in intact Caenorhabditis elegans.</description>
<content:encoded><![CDATA[

<p>
<b>Optical interrogation of neural circuits in Caenorhabditis elegans</b>
</p>
<p>Nature Methods 6, 891 (2009). <a href="http://dx.doi.org/10.1038/nmeth.1397">doi:10.1038/nmeth.1397</a>
</p>
<p>Authors: Zengcai V Guo, Anne C Hart &amp; Sharad Ramanathan</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/EkWt5xM_JdU" height="1" width="1"/>]]></content:encoded>
<dc:title>Optical interrogation of neural circuits in Caenorhabditis elegans</dc:title>
<dc:creator>Zengcai V Guo</dc:creator>
<dc:creator>Anne C Hart</dc:creator>
<dc:creator>Sharad Ramanathan</dc:creator>
<dc:identifier>doi:10.1038/nmeth.1397</dc:identifier>
<dc:source>Nature Methods 6, 891 (2009)</dc:source>
<dc:date>2009-11-08</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-08</prism:publicationDate>
<prism:doi>10.1038/nmeth.1397</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth.1397</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Article</prism:section>
<prism:startingPage>891</prism:startingPage>
<prism:endingPage>896</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth.1397</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth.1402">
<title>Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/-C3PqXqmJ9U/nmeth.1402</link>
<description>Tissue-specific expression of microRNA sponges allows precise regulation of microRNA activity in living flies. The authors investigate the role of miR-8 in the formation of neuromuscular junctions in detail.</description>
<content:encoded><![CDATA[

<p>
<b>Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms</b>
</p>
<p>Nature Methods 6, 897 (2009). <a href="http://dx.doi.org/10.1038/nmeth.1402">doi:10.1038/nmeth.1402</a>
</p>
<p>Authors: Carlos M Loya, Cecilia S Lu, David Van Vactor &amp; Tudor A Fulga</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/-C3PqXqmJ9U" height="1" width="1"/>]]></content:encoded>
<dc:title>Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms</dc:title>
<dc:creator>Carlos M Loya</dc:creator>
<dc:creator>Cecilia S Lu</dc:creator>
<dc:creator>David Van Vactor</dc:creator>
<dc:creator>Tudor A Fulga</dc:creator>
<dc:identifier>doi:10.1038/nmeth.1402</dc:identifier>
<dc:source>Nature Methods 6, 897 (2009)</dc:source>
<dc:date>2009-11-15</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-15</prism:publicationDate>
<prism:doi>10.1038/nmeth.1402</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth.1402</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Article</prism:section>
<prism:startingPage>897</prism:startingPage>
<prism:endingPage>903</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth.1402</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth.1400">
<title>Cell stimulation with optically manipulated microsources</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/MIJNB5XVPtY/nmeth.1400</link>
<description>Microsources positioned with holographic optical tweezers can establish a highly localized, three-dimensional chemical gradient that allows the manipulation of polarization and migration in single cells.</description>
<content:encoded><![CDATA[

<p>
<b>Cell stimulation with optically manipulated microsources</b>
</p>
<p>Nature Methods 6, 905 (2009). <a href="http://dx.doi.org/10.1038/nmeth.1400">doi:10.1038/nmeth.1400</a>
</p>
<p>Authors: Holger Kress, Jin-Gyu Park, Cecile O Mejean, Jason D Forster, Jason Park, Spencer S Walse, Yong Zhang, Dianqing Wu, Orion D Weiner, Tarek M Fahmy &amp; Eric R Dufresne</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/MIJNB5XVPtY" height="1" width="1"/>]]></content:encoded>
<dc:title>Cell stimulation with optically manipulated microsources</dc:title>
<dc:creator>Holger Kress</dc:creator>
<dc:creator>Jin-Gyu Park</dc:creator>
<dc:creator>Cecile O Mejean</dc:creator>
<dc:creator>Jason D Forster</dc:creator>
<dc:creator>Jason Park</dc:creator>
<dc:creator>Spencer S Walse</dc:creator>
<dc:creator>Yong Zhang</dc:creator>
<dc:creator>Dianqing Wu</dc:creator>
<dc:creator>Orion D Weiner</dc:creator>
<dc:creator>Tarek M Fahmy</dc:creator>
<dc:creator>Eric R Dufresne</dc:creator>
<dc:identifier>doi:10.1038/nmeth.1400</dc:identifier>
<dc:source>Nature Methods 6, 905 (2009)</dc:source>
<dc:date>2009-11-15</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-15</prism:publicationDate>
<prism:doi>10.1038/nmeth.1400</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth.1400</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Article</prism:section>
<prism:startingPage>905</prism:startingPage>
<prism:endingPage>909</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth.1400</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth.1396">
<title>Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/BFondmOeuaI/nmeth.1396</link>
<description>Methods for automated fluorescence imaging allow high-throughput examination of reporter expression patterns in zebrafish embryos. They are applied to mapping promoter-enhancer interactions in this organism.</description>
<content:encoded><![CDATA[

<p>
<b>Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos</b>
</p>
<p>Nature Methods 6, 911 (2009). <a href="http://dx.doi.org/10.1038/nmeth.1396">doi:10.1038/nmeth.1396</a>
</p>
<p>Authors: Jochen Gehrig, Markus Reischl, &#201;va Kalm&#225;r, Marco Ferg, Yavor Hadzhiev, Andreas Zaucker, Chengyi Song, Simone Schindler, Urban Liebel &amp; Ferenc M&#252;ller</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/BFondmOeuaI" height="1" width="1"/>]]></content:encoded>
<dc:title>Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos</dc:title>
<dc:creator>Jochen Gehrig</dc:creator>
<dc:creator>Markus Reischl</dc:creator>
<dc:creator>Éva Kalmár</dc:creator>
<dc:creator>Marco Ferg</dc:creator>
<dc:creator>Yavor Hadzhiev</dc:creator>
<dc:creator>Andreas Zaucker</dc:creator>
<dc:creator>Chengyi Song</dc:creator>
<dc:creator>Simone Schindler</dc:creator>
<dc:creator>Urban Liebel</dc:creator>
<dc:creator>Ferenc Müller</dc:creator>
<dc:identifier>doi:10.1038/nmeth.1396</dc:identifier>
<dc:source>Nature Methods 6, 911 (2009)</dc:source>
<dc:date>2009-11-08</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-08</prism:publicationDate>
<prism:doi>10.1038/nmeth.1396</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth.1396</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Article</prism:section>
<prism:startingPage>911</prism:startingPage>
<prism:endingPage>916</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth.1396</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth.1401">
<title>An auxin-based degron system for the rapid depletion of proteins in nonplant cells</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/ua8lLmIFch0/nmeth.1401</link>
<description>A degradation pathway found in plants, dependent on the hormone auxin, can be transplanted and harnessed to induce rapid and reversible target protein degradation in both yeast and animal cells.</description>
<content:encoded><![CDATA[

<p>
<b>An auxin-based degron system for the rapid depletion of proteins in nonplant cells</b>
</p>
<p>Nature Methods 6, 917 (2009). <a href="http://dx.doi.org/10.1038/nmeth.1401">doi:10.1038/nmeth.1401</a>
</p>
<p>Authors: Kohei Nishimura, Tatsuo Fukagawa, Haruhiko Takisawa, Tatsuo Kakimoto &amp; Masato Kanemaki</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/ua8lLmIFch0" height="1" width="1"/>]]></content:encoded>
<dc:title>An auxin-based degron system for the rapid depletion of proteins in nonplant cells</dc:title>
<dc:creator>Kohei Nishimura</dc:creator>
<dc:creator>Tatsuo Fukagawa</dc:creator>
<dc:creator>Haruhiko Takisawa</dc:creator>
<dc:creator>Tatsuo Kakimoto</dc:creator>
<dc:creator>Masato Kanemaki</dc:creator>
<dc:identifier>doi:10.1038/nmeth.1401</dc:identifier>
<dc:source>Nature Methods 6, 917 (2009)</dc:source>
<dc:date>2009-11-15</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-15</prism:publicationDate>
<prism:doi>10.1038/nmeth.1401</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth.1401</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Article</prism:section>
<prism:startingPage>917</prism:startingPage>
<prism:endingPage>922</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth.1401</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth.1395">
<title>High-speed nanoscopic tracking of the position and orientation of a single virus</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/XhQMJdVtKdI/nmeth.1395</link>
<description>A combination of scattering interferometry and single-molecule fluorescence microscopy allows visualization of both the position and orientation of single Simian virus 40 particles on lipid bilayers and provides evidence of viral interaction with receptors in membrane nanodomains.</description>
<content:encoded><![CDATA[

<p>
<b>High-speed nanoscopic tracking of the position and orientation of a single virus</b>
</p>
<p>Nature Methods 6, 923 (2009). <a href="http://dx.doi.org/10.1038/nmeth.1395">doi:10.1038/nmeth.1395</a>
</p>
<p>Authors: Philipp Kukura, Helge Ewers, Christian M&#252;ller, Alois Renn, Ari Helenius &amp; Vahid Sandoghdar</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/XhQMJdVtKdI" height="1" width="1"/>]]></content:encoded>
<dc:title>High-speed nanoscopic tracking of the position and orientation of a single virus</dc:title>
<dc:creator>Philipp Kukura</dc:creator>
<dc:creator>Helge Ewers</dc:creator>
<dc:creator>Christian Müller</dc:creator>
<dc:creator>Alois Renn</dc:creator>
<dc:creator>Ari Helenius</dc:creator>
<dc:creator>Vahid Sandoghdar</dc:creator>
<dc:identifier>doi:10.1038/nmeth.1395</dc:identifier>
<dc:source>Nature Methods 6, 923 (2009)</dc:source>
<dc:date>2009-11-01</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-01</prism:publicationDate>
<prism:doi>10.1038/nmeth.1395</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth.1395</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Article</prism:section>
<prism:startingPage>923</prism:startingPage>
<prism:endingPage>927</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth.1395</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth1209-929">
<title>GPCRs: insane in the membrane</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/e4jb3sMK1ks/nmeth1209-929</link>
<description>They are the quintessential drug target—but the dynamic structures and highly elaborate mechanisms of G protein–coupled receptors continue to keep experts in both industry and academia on their toes.</description>
<content:encoded><![CDATA[

<p>
<b>GPCRs: insane in the membrane</b>
</p>
<p>Nature Methods 6, 929 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-929">doi:10.1038/nmeth1209-929</a>
</p>
<p>Author: Michael Eisenstein</p>
<p>They are the quintessential drug target&#8212;but the dynamic structures and highly elaborate mechanisms of G protein&#8211;coupled receptors continue to keep experts in both industry and academia on their toes.</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/e4jb3sMK1ks" height="1" width="1"/>]]></content:encoded>
<dc:title>GPCRs: insane in the membrane</dc:title>
<dc:creator>Michael Eisenstein</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-929</dc:identifier>
<dc:source>Nature Methods 6, 929 (2009)</dc:source>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:doi>10.1038/nmeth1209-929</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth1209-929</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Technology Feature</prism:section>
<prism:startingPage>929</prism:startingPage>
<prism:endingPage>933</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth1209-929</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth1209-934">
<title>Addendum: Literature-curated protein interaction datasets</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/l-_wBTROC4U/nmeth1209-934</link>
<description />
<content:encoded><![CDATA[

<p>
<b>Addendum: Literature-curated protein interaction datasets</b>
</p>
<p>Nature Methods 6, 934 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-934">doi:10.1038/nmeth1209-934</a>
</p>
<p>Author: Michael E Cusick, Haiyuan Yu, Alex Smolyar, Kavitha Venkatesan, Anne-Ruxandra Carvunis, Nicolas Simonis, Jean-Fran&#231;ois Rual, Heather Borick, Pascal Braun, Matija Dreze, Jean Vandenhaute, Mary Galli, Junshi Yazaki, David E Hill, Joseph R Ecker, Frederick P Roth &amp; Marc Vidal</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/l-_wBTROC4U" height="1" width="1"/>]]></content:encoded>
<dc:title>Addendum: Literature-curated protein interaction datasets</dc:title>
<dc:creator>Michael E Cusick</dc:creator>
<dc:creator>Haiyuan Yu</dc:creator>
<dc:creator>Alex Smolyar</dc:creator>
<dc:creator>Kavitha Venkatesan</dc:creator>
<dc:creator>Anne-Ruxandra Carvunis</dc:creator>
<dc:creator>Nicolas Simonis</dc:creator>
<dc:creator>Jean-François Rual</dc:creator>
<dc:creator>Heather Borick</dc:creator>
<dc:creator>Pascal Braun</dc:creator>
<dc:creator>Matija Dreze</dc:creator>
<dc:creator>Jean Vandenhaute</dc:creator>
<dc:creator>Mary Galli</dc:creator>
<dc:creator>Junshi Yazaki</dc:creator>
<dc:creator>David E Hill</dc:creator>
<dc:creator>Joseph R Ecker</dc:creator>
<dc:creator>Frederick P Roth</dc:creator>
<dc:creator>Marc Vidal</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-934</dc:identifier>
<dc:source>Nature Methods 6, 934 (2009)</dc:source>
<dc:date>2009-11-25</dc:date>
<prism:publicationName>Nature Methods</prism:publicationName>
<prism:publicationDate>2009-11-25</prism:publicationDate>
<prism:doi>10.1038/nmeth1209-934</prism:doi>
<prism:url>http://dx.doi.org/10.1038/nmeth1209-934</prism:url>
<prism:volume>6</prism:volume>
<prism:number>12</prism:number>
<prism:section>Addendum</prism:section>
<prism:startingPage>934</prism:startingPage>
<prism:endingPage>935</prism:endingPage>
<feedburner:origLink>http://dx.doi.org/10.1038/nmeth1209-934</feedburner:origLink></item>
<item rdf:about="http://dx.doi.org/10.1038/nmeth1209-935a">
<title>Corrigendum: Nanoscale live-cell imaging using hopping probe ion conductance microscopy</title>
<link>http://feeds.nature.com/~r/nmeth/rss/current/~3/G3fIf05PMqM/nmeth1209-935a</link>
<description />
<content:encoded><![CDATA[

<p>
<b>Corrigendum: Nanoscale live-cell imaging using hopping probe ion conductance microscopy</b>
</p>
<p>Nature Methods 6, 935 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-935a">doi:10.1038/nmeth1209-935a</a>
</p>
<p>Author: Pavel Novak, Chao Li, Andrew I Shevchuk, Ruben Stepanyan, Matthew Caldwell, Simon Hughes, Trevor G Smart, Julia Gorelik, Victor P Ostanin, Max J Lab, Guy W J Moss, Gregory I Frolenkov, David Klenerman &amp; Yuri E Korchev</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/G3fIf05PMqM" height="1" width="1"/>]]></content:encoded>
<dc:title>Corrigendum: Nanoscale live-cell imaging using hopping probe ion conductance microscopy</dc:title>
<dc:creator>Pavel Novak</dc:creator>
<dc:creator>Chao Li</dc:creator>
<dc:creator>Andrew I Shevchuk</dc:creator>
<dc:creator>Ruben Stepanyan</dc:creator>
<dc:creator>Matthew Caldwell</dc:creator>
<dc:creator>Simon Hughes</dc:creator>
<dc:creator>Trevor G Smart</dc:creator>
<dc:creator>Julia Gorelik</dc:creator>
<dc:creator>Victor P Ostanin</dc:creator>
<dc:creator>Max J Lab</dc:creator>
<dc:creator>Guy W J Moss</dc:creator>
<dc:creator>Gregory I Frolenkov</dc:creator>
<dc:creator>David Klenerman</dc:creator>
<dc:creator>Yuri E Korchev</dc:creator>
<dc:identifier>doi:10.1038/nmeth1209-935a</dc:identifier>
<dc:source>Nature Methods 6, 935 (2009)</dc:source>
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<b>Erratum: 'Edgetic' perturbation of a C. elegans BCL2 ortholog</b>
</p>
<p>Nature Methods 6, 935 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-935b">doi:10.1038/nmeth1209-935b</a>
</p>
<p>Author: Matija Dreze, Benoit Charloteaux, Stuart Milstein, Pierre-Olivier Vidalain, Muhammed A Yildirim, Quan Zhong, Nenad Svrzikapa, Viviana Romero, G&#233;raldine Laloux, Robert Brasseur, Jean Vandenhaute, Mike Boxem, Michael E Cusick, David E Hill &amp; Marc Vidal</p>
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<dc:title>Erratum: 'Edgetic' perturbation of a C. elegans BCL2 ortholog</dc:title>
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<dc:creator>Benoit Charloteaux</dc:creator>
<dc:creator>Stuart Milstein</dc:creator>
<dc:creator>Pierre-Olivier Vidalain</dc:creator>
<dc:creator>Muhammed A Yildirim</dc:creator>
<dc:creator>Quan Zhong</dc:creator>
<dc:creator>Nenad Svrzikapa</dc:creator>
<dc:creator>Viviana Romero</dc:creator>
<dc:creator>Géraldine Laloux</dc:creator>
<dc:creator>Robert Brasseur</dc:creator>
<dc:creator>Jean Vandenhaute</dc:creator>
<dc:creator>Mike Boxem</dc:creator>
<dc:creator>Michael E Cusick</dc:creator>
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<dc:identifier>doi:10.1038/nmeth1209-935b</dc:identifier>
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<title>Erratum: What's in a test?</title>
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<p>
<b>Erratum: What's in a test?</b>
</p>
<p>Nature Methods 6, 935 (2009). <a href="http://dx.doi.org/10.1038/nmeth1209-935c">doi:10.1038/nmeth1209-935c</a>
</p>
<p>Author: Anonymous</p>
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<dc:title>Erratum: What's in a test?</dc:title>
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<dc:identifier>doi:10.1038/nmeth1209-935c</dc:identifier>
<dc:source>Nature Methods 6, 935 (2009)</dc:source>
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<title>A cell-permeant dye for cell cycle analysis by flow and laser-scanning microplate cytometry</title>
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<p>
<b>A cell-permeant dye for cell cycle analysis by flow and laser-scanning microplate cytometry</b>
</p>
<p>Nature Methods 6, (2009). <a href="http://dx.doi.org/">doi:</a>
</p>
<p>Authors: YueJun Xiang, Hilary Cox, Irina Lebedeva, Jack Coleman, Dee Shen, Praveen Pande, Joanne Schultz &amp; Wayne F Patton</p>
<img src="http://feeds.feedburner.com/~r/nmeth/rss/current/~4/ejaviLK4zkc" height="1" width="1"/>]]></content:encoded>
<dc:title>A cell-permeant dye for cell cycle analysis by flow and laser-scanning microplate cytometry</dc:title>
<dc:creator>YueJun Xiang</dc:creator>
<dc:creator>Hilary Cox</dc:creator>
<dc:creator>Irina Lebedeva</dc:creator>
<dc:creator>Jack Coleman</dc:creator>
<dc:creator>Dee Shen</dc:creator>
<dc:creator>Praveen Pande</dc:creator>
<dc:creator>Joanne Schultz</dc:creator>
<dc:creator>Wayne F Patton</dc:creator>
<dc:source>Nature Methods 6, (2009)</dc:source>
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<title>Carestream Molecular Imaging: imaging of cancer biology and relevant pathways in vivo</title>
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<p>
<b>Carestream Molecular Imaging: imaging of cancer biology and relevant pathways in vivo</b>
</p>
<p>Nature Methods 6, (2009). <a href="http://dx.doi.org/">doi:</a>
</p>
<p>Authors: Seth T Gammon, Warren M Leevy &amp; Merrill Loechner</p>
]]></content:encoded>
<dc:title>Carestream Molecular Imaging: imaging of cancer biology and relevant pathways in vivo</dc:title>
<dc:creator>Seth T Gammon</dc:creator>
<dc:creator>Warren M Leevy</dc:creator>
<dc:creator>Merrill Loechner</dc:creator>
<dc:source>Nature Methods 6, (2009)</dc:source>
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<title>New benchtop bioreactor uses presterilized, prevalidated, single-use vessels to simplify cell culture</title>
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<p>
<b>New benchtop bioreactor uses presterilized, prevalidated, single-use vessels to simplify cell culture</b>
</p>
<p>Nature Methods 6, (2009). <a href="http://dx.doi.org/">doi:</a>
</p>
<p>Authors: Guozheng Wang, Wenying Zhang &amp; Rich Mirro</p>
]]></content:encoded>
<dc:title>New benchtop bioreactor uses presterilized, prevalidated, single-use vessels to simplify cell culture</dc:title>
<dc:creator>Guozheng Wang</dc:creator>
<dc:creator>Wenying Zhang</dc:creator>
<dc:creator>Rich Mirro</dc:creator>
<dc:source>Nature Methods 6, (2009)</dc:source>
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<title>CERTOMAT® CTplus: a purpose-built incubator shaker for mammalian cell cultures</title>
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<p>
<b>CERTOMAT&#174; CTplus: a purpose-built incubator shaker for mammalian cell cultures</b>
</p>
<p>Nature Methods 6, (2009). <a href="http://dx.doi.org/">doi:</a>
</p>
<p>Author: Wolfgang Asche</p>
]]></content:encoded>
<dc:title>CERTOMAT® CTplus: a purpose-built incubator shaker for mammalian cell cultures</dc:title>
<dc:creator>Wolfgang Asche</dc:creator>
<dc:source>Nature Methods 6, (2009)</dc:source>
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<title>New adjuvants for accelerated and enhanced antibody response</title>
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<b>New adjuvants for accelerated and enhanced antibody response</b>
</p>
<p>Nature Methods 6, (2009). <a href="http://dx.doi.org/">doi:</a>
</p>
<p>Author: Sergey Litvinov</p>
]]></content:encoded>
<dc:title>New adjuvants for accelerated and enhanced antibody response</dc:title>
<dc:creator>Sergey Litvinov</dc:creator>
<dc:source>Nature Methods 6, (2009)</dc:source>
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<title>RNA amplification and cDNA synthesis for qRT-PCR directly from a single cell</title>
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<p>
<b>RNA amplification and cDNA synthesis for qRT-PCR directly from a single cell</b>
</p>
<p>Nature Methods 6, (2009). <a href="http://dx.doi.org/">doi:</a>
</p>
<p>Authors: Judith E Meis &amp; Anupama Khanna</p>
]]></content:encoded>
<dc:title>RNA amplification and cDNA synthesis for qRT-PCR directly from a single cell</dc:title>
<dc:creator>Judith E Meis</dc:creator>
<dc:creator>Anupama Khanna</dc:creator>
<dc:source>Nature Methods 6, (2009)</dc:source>
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