Nature Protocols 4, 1709 (2009). doi:10.1038/nprot.2009.159

Authors: Mardas Daneshian, Sonja von Aulock & Thomas Hartung

We present an internationally validated protocol for the evaluation of pyrogenic contaminations using human whole blood. In the in vitro pyrogen test (IPT) the sample is incubated with fresh or cryopreserved human whole blood, and the proinflammatory cytokine interleukin-1β (IL-1β) is detected by enzyme-linked

Nature Protocols 4, 1722 (2009). doi:10.1038/nprot.2009.183

Authors: Susan M Gribble, Bee Ling Ng, Elena Prigmore, Tomas Fitzgerald & Nigel P Carter

Array painting is a technique that uses microarray technology to rapidly map chromosome translocation breakpoints. Previous methods to map translocation breakpoints have used fluorescence in situ hybridization (FISH) and have consequently been labor-intensive, time-consuming and restricted to the low breakpoint resolution imposed by the

Nature Protocols 4, 1737 (2009). doi:10.1038/nprot.2009.185

Authors: Anne Limbourg, Thomas Korff, L Christian Napp, Wolfgang Schaper, Helmut Drexler & Florian P Limbourg

Blood vessel growth in adult organisms involves the following two fundamental processes: angiogenesis, the proliferation and extension of capillary networks; and arteriogenesis, the growth of functional arteries. We provide a protocol for the evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb

Nature Protocols 4, 1779 (2009). doi:10.1038/nprot.2009.190

Author: Maria A Serrat

Fluorescent microspheres are commonly used to assess bone blood supply in large animals, but the technique is not widely used in smaller mammals, as traditional methods such as reference blood sampling, ventilation and catheterization are not easily applied. This protocol describes a viable alternative for