Browse Articles

  • Brief Communication |

    MicroED structure of a peptide from the β2–α2 loop of the bank vole prion protein reveals a protofibril stabilized by a dense network of hydrogen bonds.

    • Marcus Gallagher-Jones
    • , Calina Glynn
    • , David R. Boyer
    • , Michael W. Martynowycz
    • , Evelyn Hernandez
    • , Jennifer Miao
    • , Chih-Te Zee
    • , Irina V. Novikova
    • , Lukasz Goldschmidt
    • , Heather T. McFarlane
    • , Gustavo F. Helguera
    • , James E. Evans
    • , Michael R. Sawaya
    • , Duilio Cascio
    • , David S. Eisenberg
    • , Tamir Gonen
    •  & Jose A. Rodriguez
  • Article |

    The structure of the fully assembled yeast exocyst complex, which mediates the tethering of secretory vesicles to the plasma membrane during exocytosis, provides new insights to hierarchical complex assembly and the mechanism of vesicle tethering.

    • Kunrong Mei
    • , Yan Li
    • , Shaoxiao Wang
    • , Guangcan Shao
    • , Jia Wang
    • , Yuehe Ding
    • , Guangzuo Luo
    • , Peng Yue
    • , Jun-Jie Liu
    • , Xinquan Wang
    • , Meng-Qiu Dong
    • , Hong-Wei Wang
    •  & Wei Guo
  • Technical Report |

    The Protein Contacts Atlas is an interactive resource of non-covalent contacts that can generate multiple representations of non-covalent contacts from PDB structures at different scales, from atoms to subunits and entire complexes.

    • Melis Kayikci
    • , A. J. Venkatakrishnan
    • , James Scott-Brown
    • , Charles N. J. Ravarani
    • , Tilman Flock
    •  & M. Madan Babu
  • Review Article |

    Rossmann and colleagues review the rapid progress in our understanding of the structure of Zika virus, building on previous studies of other flaviviruses such as dengue virus.

    • S. Saif Hasan
    • , Madhumati Sevvana
    • , Richard J. Kuhn
    •  & Michael G. Rossmann
  • Review Article |

    In this Review, the authors discuss recent insights into the mechanism of GPCR signaling provided by structural and biophysical elucidation of receptor interactions with G proteins and arrestins.

    • Daniel Hilger
    • , Matthieu Masureel
    •  & Brian K. Kobilka
  • News & Views |

    Chd1 is a highly conserved chromatin remodeler found across all eukaryotic species. A recent study shows the structural changes that take place when yeast Chd1 binds to its nucleosomal substrate and reveals how they relate to remodeler function.

    • Michaela M. Smolle
  • Editorial |

    This January 2018 issue starts the 25th year of NSMB’s journey. We mark the occasion by launching a special series that celebrates the exciting research uncovering the fundamental principles behind biological processes.

  • Article |

    During oogenesis, H3K4 trimethylation is targeted to genomic elements through transcription-dependent and transcription-independent mechanisms, the latter relying on MLL2 recruitment to unmethylated CpG-rich regions.

    • Courtney W. Hanna
    • , Aaron Taudt
    • , Jiahao Huang
    • , Lenka Gahurova
    • , Andrea Kranz
    • , Simon Andrews
    • , Wendy Dean
    • , A. Francis Stewart
    • , Maria Colomé-Tatché
    •  & Gavin Kelsey
  • Article |

    The cryo-EM structure of the full-length TRPV5 channel in complex with inhibitor econazole reveals a domain-swapped architecture and provides insights into mechanisms of inhibition.

    • Taylor E. T. Hughes
    • , David T. Lodowski
    • , Kevin W. Huynh
    • , Aysenur Yazici
    • , John Del Rosario
    • , Abhijeet Kapoor
    • , Sandip Basak
    • , Amrita Samanta
    • , Xu Han
    • , Sudha Chakrapani
    • , Z. Hong Zhou
    • , Marta Filizola
    • , Tibor Rohacs
    • , Seungil Han
    •  & Vera Y. Moiseenkova-Bell
  • Article |

    The ER-resident Hsp70 BiP is regulated by NEF Bap. The interactions between BiP and Bap are now dissected using biochemistry, molecular modeling and smFRET approaches, revealing that Bap affects both domains of BiP, to coordinate release of substrate and nucleotide.

    • Mathias Rosam
    • , Daniela Krader
    • , Christina Nickels
    • , Janine Hochmair
    • , Katrin C. Back
    • , Ganesh Agam
    • , Anders Barth
    • , Cathleen Zeymer
    • , Jelle Hendrix
    • , Markus Schneider
    • , Iris Antes
    • , Jochen Reinstein
    • , Don C. Lamb
    •  & Johannes Buchner
  • Article |

    The APOBEC-AID family of cytidine deaminases target single-stranded nucleic acids for cytidine-to-uridine deamination and can thereby affect DNA repair processes that occur during CRISPR–Cas9-mediated genome editing.

    • Liqun Lei
    • , Hongquan Chen
    • , Wei Xue
    • , Bei Yang
    • , Bian Hu
    • , Jia Wei
    • , Lijie Wang
    • , Yiqiang Cui
    • , Wei Li
    • , Jianying Wang
    • , Lei Yan
    • , Wanjing Shang
    • , Jimin Gao
    • , Jiahao Sha
    • , Min Zhuang
    • , Xingxu Huang
    • , Bin Shen
    • , Li Yang
    •  & Jia Chen
  • Article |

    Heterozygous cancer-associated SMARCA4 missense mutations disrupt conserved ATPase surfaces of this chromatin remodeler and alter the open chromatin landscape, thus inducing pro-oncogenic gene expression.

    • H. Courtney Hodges
    • , Benjamin Z. Stanton
    • , Katerina Cermakova
    • , Chiung-Ying Chang
    • , Erik L. Miller
    • , Jacob G. Kirkland
    • , Wai Lim Ku
    • , Vaclav Veverka
    • , Keji Zhao
    •  & Gerald R. Crabtree
  • Article |

    Cryo-EM structures of nucleosomes in partially unwrapped, transient states reveal intrinsic plasticity that is required for nucleosome stability and can be exploited by chromatin-remodeling factors.

    • Silvija Bilokapic
    • , Mike Strauss
    •  & Mario Halic
  • News & Views |

    The helicase intrinsic to DNA polymerase θ (Polθ), the versatile mediator of microhomology-based repair of DNA double-strand breaks and stalled replication forks, is now revealed to be a member of an elite group of proteins known as annealing helicases. This small family of enzymes remodels DNA intermediates in multiple repair processes that are crucial to preserving genome stability and warding off cancer and aging.

    • Judith L Campbell
    •  & Hongzhi Li
  • News & Views |

    The crystal structure of an oligosaccharyltransferase in complex with a sugar donor and an acceptor peptide provides insight into the mechanism of protein glycosylation and reveals how lipid-linked oligosaccharides are positioned in the enzyme active site.

    • Shiteshu Shrimal
    • , Natalia A Cherepanova
    •  & Reid Gilmore
  • Perspective |

    The mechanics and mechanisms of ribosomal translocation, including the conformational rearrangements in the ribosome and the roles of EF-G and tRNAs, are discussed in this Perspective by Mohan, Noller and colleagues.

    • Harry F Noller
    • , Laura Lancaster
    • , Jie Zhou
    •  & Srividya Mohan
  • News & Views |

    Deadenylation of mRNAs is generally associated with translational inhibition and mRNA decay. A study now reports that, unexpectedly, highly expressed genes tend to have shorter poly(A) tails and suggests that poly(A) tails can be 'pruned', generating a 30-nucleotide-biased phased distribution, likely due to protection by poly(A)-binding proteins.

    • Luciana A Castellano
    •  & Ariel A Bazzini
  • Article |

    The cryo-EM structure of the human INO80 chromatin-remodeling complex reveals the architecture of the complex centered around a RUVBL1–2 AAA+ heterohexamer.

    • Ricardo J. Aramayo
    • , Oliver Willhoft
    • , Rafael Ayala
    • , Rohan Bythell-Douglas
    • , Dale B. Wigley
    •  & Xiaodong Zhang
  • Article |

    Ultrastructural analysis of nuclear membrane topology and assembling NPCs reveals how mitotic cells can rapidly establish a closed nuclear compartment while at the same time making it transport competent.

    • Shotaro Otsuka
    • , Anna M. Steyer
    • , Martin Schorb
    • , Jean-Karim Hériché
    • , M. Julius Hossain
    • , Suruchi Sethi
    • , Moritz Kueblbeck
    • , Yannick Schwab
    • , Martin Beck
    •  & Jan Ellenberg
  • Article |

    Apolipoprotein A-I (apoA-I) is the scaffold protein that is essential for the assembly and function of HDL particles. A structural model for monomeric, lipid-free apoA-I, based on previous and new data, is now presented.

    • John T Melchior
    • , Ryan G Walker
    • , Allison L Cooke
    • , Jamie Morris
    • , Mark Castleberry
    • , Thomas B Thompson
    • , Martin K Jones
    • , Hyun D Song
    • , Kerry-Anne Rye
    • , Michael N Oda
    • , Mary G Sorci-Thomas
    • , Michael J Thomas
    • , Jay W Heinecke
    • , Xiaohu Mei
    • , David Atkinson
    • , Jere P Segrest
    • , Sissel Lund-Katz
    • , Michael C Phillips
    •  & W Sean Davidson