Browse Articles

  • Article |

    Genome-wide analyses in fission yeast reveal that iron homeostasis influences facultative heterochromatin assembly to modulate gene expression in response to changes in growth temperature.

    • Pamela S. Gallagher
    • , Madeline Larkin
    • , Gobi Thillainadesan
    • , Jothy Dhakshnamoorthy
    • , Vanivilasini Balachandran
    • , Hua Xiao
    • , Christopher Wellman
    • , Raghunath Chatterjee
    • , David Wheeler
    •  & Shiv I. S. Grewal
  • Review Article |

    Developments in basic RNA biology have spawned RNA-based strategies to generate new types of therapeutics. Judy Lieberman reviews RNA-based drug design and discusses barriers to more widespread applications and possible ways to overcome them.

    • Judy Lieberman
  • Article |

    Selective disruption of adjacent splice acceptor and donor sites identifies cryptic exons involved in recursive splicing in Drosophila, thus unifying the mechanism of recursive splicing in flies and mammals.

    • Brian Joseph
    • , Shu Kondo
    •  & Eric C. Lai
  • Article |

    Single-molecule FRET analysis of DNA binding and unwinding by two engineered Cas9s reveals that their improved specificity is achieved through lower binding and more transient unwinding of mismatched DNA and lower intrinsic cleavage reaction rates.

    • Digvijay Singh
    • , Yanbo Wang
    • , John Mallon
    • , Olivia Yang
    • , Jingyi Fei
    • , Anustup Poddar
    • , Damon Ceylan
    • , Scott Bailey
    •  & Taekjip Ha
  • Article |

    Cryo-EM structures of human ABCG2 bound to synthetic derivatives of inhibitor Ko143 or the multidrug resistance modulator tariquidar together with insight into the structure–activity relationship of the Ko143 scaffold provide a basis for the design of novel ABCG2 inhibitors.

    • Scott M. Jackson
    • , Ioannis Manolaridis
    • , Julia Kowal
    • , Melanie Zechner
    • , Nicholas M. I. Taylor
    • , Manuel Bause
    • , Stefanie Bauer
    • , Ruben Bartholomaeus
    • , Guenther Bernhardt
    • , Burkhard Koenig
    • , Armin Buschauer
    • , Henning Stahlberg
    • , Karl-Heinz Altmann
    •  & Kaspar P. Locher
  • Author Correction |

    • Jocelyn Charlton
    • , Timothy L. Downing
    • , Zachary D. Smith
    • , Hongcang Gu
    • , Kendell Clement
    • , Ramona Pop
    • , Veronika Akopian
    • , Sven Klages
    • , David P. Santos
    • , Alexander M. Tsankov
    • , Bernd Timmermann
    • , Michael J. Ziller
    • , Evangelos Kiskinis
    • , Andreas Gnirke
    •  & Alexander Meissner
  • News & Views |

    Recent developments in transcriptome-wide sequencing technologies have enabled the identification of cellular mRNA decay intermediates. Although canonical mRNA decay has been shown to occur by deadenylation followed by decapping and subsequent exonucleolytic decay from both mRNA ends, a study by Mourelatos and colleagues now defines mRNA fragments that are generated on polysomes by endonucleolytic cleavages phased by the associated ribosome.

    • Tatsuaki Kurosaki
    •  & Lynne E. Maquat
  • Article |

    A lag in nascent strand DNA methylation contributes to heterogeneous methylation in asynchronous cell populations, but cancer cells and active transcription factor binding sites preserve heterogeneity even after cell cycle arrest.

    • Jocelyn Charlton
    • , Timothy L. Downing
    • , Zachary D. Smith
    • , Hongcang Gu
    • , Kendell Clement
    • , Ramona Pop
    • , Veronika Akopian
    • , Sven Klages
    • , David P. Santos
    • , Alexander M. Tsankov
    • , Bernd Timmermann
    • , Michael J. Ziller
    • , Evangelos Kiskinis
    • , Andreas Gnirke
    •  & Alexander Meissner
  • News & Views |

    Nanobodies have emerged as highly versatile and useful binding molecules in biomedical research. A technical report describes a cost- and time-effective in vitro platform that facilitates the generation of desired nanobodies, including conformationally selective nanobodies against agonist-bound G-protein-coupled receptors (GPCRs).

    • Ulrich Rothbauer
  • News & Views |

    Inheritance of Polycomb repressive complex 2 (PRC2)-mediated gene silencing involves self-propagation of histone H3 lysine 27 (H3K27) methylation from an initial nucleation site, but how the first H3K27 methylation marks are established is not fully understood. A recent study reveals that PRC2 can reconstitute H3K27 methylation de novo in cells that have lost the mark. This reconstitution is dependent on the PRC2 core component SUZ12, which provides a novel link between initiation and self-propagation of this critical epigenetic mark.

    • Jafar Sharif
    •  & Haruhiko Koseki
  • News & Views |

    A new study reveals how the oocyte-specific transcription factor TAp63 ensures female germ line fidelity and describes approaches to circumvent premature ovarian insufficiency in women receiving cytotoxic chemotherapy.

    • Wa Xian
    •  & Frank McKeon
  • News & Views |

    Transcripts with highly complementary sequences can target microRNAs (miRNAs) for degradation, but the physiological relevance of target-directed miRNA degradation (TDMD) has remained unclear. Bitetti et al. now identify a conserved vertebrate RNA that induces TDMD in the cerebellum of zebrafish and mouse to promote wild-type animal behaviors.

    • Manuel de la Mata
    •  & Helge Großhans
  • Article |

    A platform for high-throughput expression screening and large-scale production of cystine-dense peptides, alongside a global structure-based classification, is presented.

    • Colin E. Correnti
    • , Mesfin M. Gewe
    • , Christopher Mehlin
    • , Ashok D. Bandaranayake
    • , William A. Johnsen
    • , Peter B. Rupert
    • , Mi-Youn Brusniak
    • , Midori Clarke
    • , Skyler E. Burke
    • , Willem De Van Der Schueren
    • , Kristina Pilat
    • , Shanon M. Turnbaugh
    • , Damon May
    • , Alex Watson
    • , Man Kid Chan
    • , Christopher D. Bahl
    • , James M. Olson
    •  & Roland K. Strong
  • Article |

    RNA-directed miRNA degradation triggered by a brain-specific genome-encoded transcript regulates explorative and anxiety-like behavior in zebrafish and affects balance and motor learning in mice.

    • Angelo Bitetti
    • , Allison C. Mallory
    • , Elisabetta Golini
    • , Claudia Carrieri
    • , Héctor Carreño Gutiérrez
    • , Emerald Perlas
    • , Yuvia A. Pérez-Rico
    • , Glauco P. Tocchini-Valentini
    • , Anton J. Enright
    • , William H. J. Norton
    • , Silvia Mandillo
    • , Dónal O’Carroll
    •  & Alena Shkumatava
  • Article |

    Although mechanisms wherein pre-existing H3K27 methylation directs recruitment of PRC2 to support its own maintenance have been proposed, H3K27 methylation patterns in mESCs are not dependent on self-autonomous epigenetic inheritance.

    • Jonas W. Højfeldt
    • , Anne Laugesen
    • , Berthe M. Willumsen
    • , Helene Damhofer
    • , Lin Hedehus
    • , Andrey Tvardovskiy
    • , Faizaan Mohammad
    • , Ole N. Jensen
    •  & Kristian Helin
  • Article |

    Structural analyses of Xenopus tropicalis TRPV4 reveal a single ion-binding site in the selectivity filter and distinct interactions between the voltage-sensor-like and pore domains and provide a blueprint to understanding disease-related mutations.

    • Zengqin Deng
    • , Navid Paknejad
    • , Grigory Maksaev
    • , Monica Sala-Rabanal
    • , Colin G. Nichols
    • , Richard K. Hite
    •  & Peng Yuan
  • Article |

    p63 activation in response to DNA damage leads to oocyte death and loss of fertility in women receiving chemotherapy. Activation requires sequential phosphorylation by CHK2 and CK1 kinases, and inhibition of these kinases rescues oocytes from apoptosis induced by chemotherapy.

    • Marcel Tuppi
    • , Sebastian Kehrloesser
    • , Daniel W. Coutandin
    • , Valerio Rossi
    • , Laura M. Luh
    • , Alexander Strubel
    • , Katharina Hötte
    • , Meike Hoffmeister
    • , Birgit Schäfer
    • , Tiago De Oliveira
    • , Florian Greten
    • , Ernst H. K. Stelzer
    • , Stefan Knapp
    • , Massimo De Felici
    • , Christian Behrends
    • , Francesca Gioia Klinger
    •  & Volker Dötsch