Browse Articles

  • Article |

    CD28 signaling motifs are sequestered within the membrane via interactions with phospholipids. TCR activation increases the local Ca2+ concentration, which disrupts CD28-lipid interactions.

    • Wei Yang
    • , Weiling Pan
    • , Shuokai Chen
    • , Nicola Trendel
    • , Shutan Jiang
    • , Feng Xiao
    • , Manman Xue
    • , Wei Wu
    • , Zeli Peng
    • , Xiaoxi Li
    • , Hongbin Ji
    • , Xiaolong Liu
    • , Hai Jiang
    • , Haopeng Wang
    • , Hongbin Shen
    • , Omer Dushek
    • , Hua Li
    •  & Chenqi Xu
  • Article |

    Histone H3 lysine 14 is propionylated and butyrylated in vivo in a metabolic-state-dependent manner and these modifications promote high levels of transcription.

    • Adam F Kebede
    • , Anna Nieborak
    • , Lara Zorro Shahidian
    • , Stephanie Le Gras
    • , Florian Richter
    • , Diana Aguilar Gómez
    • , Marijke P Baltissen
    • , Gergo Meszaros
    • , Helena de Fatima Magliarelli
    • , Aaron Taudt
    • , Raphael Margueron
    • , Maria Colomé-Tatché
    • , Romeo Ricci
    • , Sylvain Daujat
    • , Michiel Vermeulen
    • , Gerhard Mittler
    •  & Robert Schneider
  • Article |

    Biochemical and cellular analyses reveal that the helicase activity of DNA polymerase theta (Polθ) antagonizes RPA to promote DNA strand annealing and double-strand break repair via alt-NHEJ in mouse ES cells.

    • Pedro A Mateos-Gomez
    • , Tatiana Kent
    • , Sarah K Deng
    • , Shane McDevitt
    • , Ekaterina Kashkina
    • , Trung M Hoang
    • , Richard T Pomerantz
    •  & Agnel Sfeir
  • Article |

    Histone variant macroH2A1.1 inhibits PARP activity to maintain mitochondrial NAD+ pools in muscle cells, thus linking chromatin state to optimal energy metabolism.

    • Melanija Posavec Marjanović
    • , Sarah Hurtado-Bagès
    • , Maximilian Lassi
    • , Vanesa Valero
    • , Roberto Malinverni
    • , Hélène Delage
    • , Miriam Navarro
    • , David Corujo
    • , Iva Guberovic
    • , Julien Douet
    • , Pau Gama-Perez
    • , Pablo M Garcia-Roves
    • , Ivan Ahel
    • , Andreas G Ladurner
    • , Oscar Yanes
    • , Philippe Bouvet
    • , Mònica Suelves
    • , Raffaele Teperino
    • , J Andrew Pospisilik
    •  & Marcus Buschbeck
  • Article |

    Combined structural and microscopy approaches provide a model for how CAMSAP proteins recognize microtubule minus ends through their conserved CCK domains and protect microtubules from depolymerizing kinesin-13.

    • Joseph Atherton
    • , Kai Jiang
    • , Marcel M Stangier
    • , Yanzhang Luo
    • , Shasha Hua
    • , Klaartje Houben
    • , Jolien J E van Hooff
    • , Agnel-Praveen Joseph
    • , Guido Scarabelli
    • , Barry J Grant
    • , Anthony J Roberts
    • , Maya Topf
    • , Michel O Steinmetz
    • , Marc Baldus
    • , Carolyn A Moores
    •  & Anna Akhmanova
  • Article |

    Analysis of the 3D genomic organization of Schizosaccharomyces pombe during the cell cycle reveals that condensin mediates formation of large domains that serve as chromosomal compaction units, whereas cohesin forms smaller, more stable domains.

    • Hideki Tanizawa
    • , Kyoung-Dong Kim
    • , Osamu Iwasaki
    •  & Ken-ichi Noma
  • Article |

    Crystal structures and functional assays of a chimeric GABAA receptor in apo and pregnanolone-bound states reveal how neurosteroid binding alters receptor conformation to modulate channel opening.

    • Paul S Miller
    • , Suzanne Scott
    • , Simonas Masiulis
    • , Luigi De Colibus
    • , Els Pardon
    • , Jan Steyaert
    •  & A Radu Aricescu
  • News and Views |

    C-type inactivation is a process by which ion flux through a voltage-gated K+ channel is regulated at the selectivity filter. A recent structure of the Kv1.2 channel provides a view into the structural changes of the selectivity filter during C-type inactivation.

    • Francis I Valiyaveetil
  • News and Views |

    PERK is a major sensor of the unfolded protein response controlling cell fate under endoplasmic reticulum (ER) stress. A new study reveals an additional step for optimal PERK signaling, involving the binding of CNPY2 to PERK's luminal domain. The PERK–CNPY2 axis was shown to enhance cell death under ER stress in vivo influence liver disease.

    • Hery Urra
    •  & Claudio Hetz
  • News and Views |

    PCSK9 enhances LDL cholesterol (LDL-c) levels by escorting the liver LDL receptor (LDLR) to endosomes and lysosomes for degradation. PCSK9 monoclonal antibodies and RNA-antisense formulations are effective in reducing LDL cholesterol in patients. The recent structural identification of a novel pocket in PCSK9 paves the way to the future development of orally active small-molecule hypocholesterolemic drugs.

    • Nabil G Seidah
  • News and Views |

    The cellular crosstalk between different classes of regulatory noncoding RNAs has reached a new spatial dimension. Jiang et al. reveal an essential role of a nuclear-paraspeckle-organizing long noncoding RNA and its protein partners in regulating the first steps of microRNA biogenesis.

    • Jacek Krol
  • Article |

    Genome-wide analyses of the effects of U1 snRNP inhibition in human cells shows that telescripting suppresses premature cleavage and polyadenylation in long introns to sustain expression of large genes important for cell cycle and development.

    • Jung-Min Oh
    • , Chao Di
    • , Christopher C Venters
    • , Jiannan Guo
    • , Chie Arai
    • , Byung Ran So
    • , Anna Maria Pinto
    • , Zhenxi Zhang
    • , Lili Wan
    • , Ihab Younis
    •  & Gideon Dreyfuss
  • Analysis |

    Genome-wide analyses of somatic mutations across six cancer types show that mutation frequencies differ between chromosomal regions located at the nuclear core versus the periphery, and thus mutational patterns are influenced by nuclear architecture.

    • Kyle S Smith
    • , Lin L Liu
    • , Shridar Ganesan
    • , Franziska Michor
    •  & Subhajyoti De
  • Article |

    The near-atomic structure of the Chaetomium thermophilum 90S preribosome explains how assembly factors and pre-rRNA guide folding of pre-40S domains and suggests a proofreading model for the 90S–pre-40S transition.

    • Jingdong Cheng
    • , Nikola Kellner
    • , Otto Berninghausen
    • , Ed Hurt
    •  & Roland Beckmann
  • Article |

    The structure of zebrafish DUB USP30 in complex with a Lys6-linked diUb, along with biochemistry analyses, reveals the basis for Lys6-linkage specificity.

    • Yusuke Sato
    • , Kei Okatsu
    • , Yasushi Saeki
    • , Koji Yamano
    • , Noriyuki Matsuda
    • , Ai Kaiho
    • , Atsushi Yamagata
    • , Sakurako Goto-Ito
    • , Minoru Ishikawa
    • , Yuichi Hashimoto
    • , Keiji Tanaka
    •  & Shuya Fukai
  • Article |

    Structural and biochemical analyses of human USP30 explain basis for Lys6-linkage preference and regulation by PINK1 and Parkin, shedding light onto how USP30 can act as a brake on mitophagy.

    • Malte Gersch
    • , Christina Gladkova
    • , Alexander F Schubert
    • , Martin A Michel
    • , Sarah Maslen
    •  & David Komander
  • Article |

    The 3.8-Å cryo-EM structure of the Saccharomyces cerevisiae small-subunit processome in a state that precedes pre-rRNA cleavage at site A1 provides an essentially complete near-atomic model of this assembly.

    • Jonas Barandun
    • , Malik Chaker-Margot
    • , Mirjam Hunziker
    • , Kelly R Molloy
    • , Brian T Chait
    •  & Sebastian Klinge