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Article
| Open Access
Membraneless channels sieve cations in ammonia-oxidizing marine archaea
The Nitrosopumilus maritimus surface layer (S-layer) concentrates ammonium ions on its cell-facing side, acting as a multichannel sieve on the cell membrane.
- Andriko von Kügelgen
- , C. Keith Cassidy
- & Tanmay A. M. Bharat
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Review Article |
Bridging structural and cell biology with cryo-electron microscopy
The interplay between cryo-electron microscopy and cryo-electron tomography to define complex macromolecular assemblies and visualize them in situ is explored.
- Eva Nogales
- & Julia Mahamid
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Article
| Open Access
HIV-1 Env trimers asymmetrically engage CD4 receptors in membranes
HIV-1 Env trimers bound to one, two and three CD4 molecules are observed at membrane–membrane interfaces between HIV-1 and CD4-decorated virus-like particles.
- Wenwei Li
- , Zhuan Qin
- & Walther Mothes
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Article
| Open Access
Structure of the native myosin filament in the relaxed cardiac sarcomere
A cryo-electron tomography study reports the structure of thick myosin filaments of mouse cardiac muscle in the relaxed state in situ and the MyBP-C links that connect them with the surrounding thin actin filaments.
- Davide Tamborrini
- , Zhexin Wang
- & Stefan Raunser
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Article |
In situ architecture of the ER–mitochondria encounter structure
Integrative structural biology combining quantitative live imaging, cryo-correlative microscopy, subtomogram averaging and molecular modelling enables in situ determination of the structure of the endoplasmic reticulum–mitochondria encounter complex in yeast.
- Michael R. Wozny
- , Andrea Di Luca
- & Wanda Kukulski
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Article |
mRNA recognition and packaging by the human transcription–export complex
Cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNA ribonucleoprotein complexes bound to the transcription–export complex reveal how mRNAs are packaged and recognized for nuclear export.
- Belén Pacheco-Fiallos
- , Matthias K. Vorländer
- & Clemens Plaschka
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Article
| Open Access
Structural basis of mitochondrial membrane bending by the I–II–III2–IV2 supercomplex
A supercomplex comprising all four respiratory chain components contributes to the induction of mitochondrial membrane curvature and tubulation of cristae.
- Alexander Mühleip
- , Rasmus Kock Flygaard
- & Alexey Amunts
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Article |
In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex
In situ structures of PBS–PSII–PSI–LHC megacomplexes from the alga P. purpureum at near-atomic resolution using cryogenic-electron tomography and in situ single-particle analysis are reported, providing interaction details between PBS, PSII and PSI.
- Xin You
- , Xing Zhang
- & Sen-Fang Sui
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Article
| Open Access
Visualization of translation and protein biogenesis at the ER membrane
Structural studies of the ribosome-associated endoplasmic reticulum translocon complex based on cryo-electron tomography and molecular modelling reveal multiple intermediate states and interactions between the components of the complex and its cofactors.
- Max Gemmer
- , Marten L. Chaillet
- & Friedrich Förster
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Article
| Open Access
Visualizing translation dynamics at atomic detail inside a bacterial cell
Cryo-electron tomography is used to reveal the structural dynamics and functional diversity of translating ribosomes in Mycoplasma pneumoniae, providing insight into the translation elongation cycle inside cells and how it is reshaped by antibiotics.
- Liang Xue
- , Swantje Lenz
- & Julia Mahamid
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Article
| Open Access
Architecture and self-assembly of the jumbo bacteriophage nuclear shell
The nucleus-like compartment formed in bacteria during infection by jumbo phage 201phi2-1 is composed of the bacteriophage protein chimallin, which can self-assemble into closed compartments in vitro.
- Thomas G. Laughlin
- , Amar Deep
- & Elizabeth Villa
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Article |
Membrane-anchored HDCR nanowires drive hydrogen-powered CO2 fixation
The cryo-electron microscopy structure of the filamentous hydrogen-dependent CO2 reductase (HDCR) enzyme from Thermoanaerobacter kivui, together with enzymatic analysis and in situ cryo-electron tomography, provides insight into the high catalytic activity of HDCR.
- Helge M. Dietrich
- , Ricardo D. Righetto
- & Jan M. Schuller
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Article
| Open Access
The cellular environment shapes the nuclear pore complex architecture
Structure of human nuclear pore complex in its cellular environment reveals a substantially dilated central channel and shows that its nucleoplasmic and cytoplasmic rings restrict channel dimensions and create membrane asymmetry at the inner ring.
- Anthony P. Schuller
- , Matthias Wojtynek
- & Thomas U. Schwartz
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Article |
The native structure of the assembled matrix protein 1 of influenza A virus
Structures of the assembled matrix protein 1 of influenza A virus in intact virus particles and of oligomers of this protein reconstituted in vitro reveal mechanisms of assembly and disassembly of influenza virus.
- Julia Peukes
- , Xiaoli Xiong
- & John A. G. Briggs
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Article |
In-cell architecture of the nuclear pore and snapshots of its turnover
In-cell structural studies in Saccharomyces cerevisiae reveal that the configuration of the Nup159 complex is a key determinant of the mRNA export function of the nuclear pore complex, and suggest a model in which nuclear pore complexes are degraded via the autophagy machinery.
- Matteo Allegretti
- , Christian E. Zimmerli
- & Martin Beck
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Article |
Structures and distributions of SARS-CoV-2 spike proteins on intact virions
Cryo-electron microscopy and tomography studies reveal the structures, conformations and distributions of spike protein trimers on intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virions and provide a basis for understanding the interactions of the spike protein with neutralizing antibodies.
- Zunlong Ke
- , Joaquin Oton
- & John A. G. Briggs
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Letter |
Visualization of clustered protocadherin neuronal self-recognition complexes
Clustered protocadherin ectodomains spontaneously assemble to form a zipper-like lattice of alternating cis and trans interactions at membrane contact sites, which probably represents their mode of function in neuronal self-recognition.
- Julia Brasch
- , Kerry M. Goodman
- & Lawrence Shapiro
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Letter |
Rubisco condensate formation by CcmM in β-carboxysome biogenesis
The structure of a Rubisco–CcmM complex sheds light on the formation of carboxysomes in cyanobacteria.
- H. Wang
- , X. Yan
- & M. Hayer-Hartl
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Letter |
Structure of the membrane-assembled retromer coat determined by cryo-electron tomography
The retromer complex (the vacuolar protein sorting heterotrimer Vps26–Vps29–Vps35) has been resolved in association with membranes and the sorting nexin protein Vps5 using cryo-electron tomography.
- Oleksiy Kovtun
- , Natalya Leneva
- & Brett M. Collins
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Letter |
Structure and assembly of the Ebola virus nucleocapsid
Application of cryo-electron tomography and subtomogram averaging to determine the structure of the Ebola virus nucleocapsid within intact viruses and recombinant nucleocapsid-like assemblies.
- William Wan
- , Larissa Kolesnikova
- & John A. G. Briggs
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Letter |
Structure of RNA polymerase I transcribing ribosomal DNA genes
Structures of budding yeast RNA polymerase I in a catalytically active conformation are presented and confirmed by visualizing processive transcription along ribosomal DNA genes; they support a general model for transcription elongation in which contracted and expanded polymerase conformations are associated with active and inactive states, respectively.
- Simon Neyer
- , Michael Kunz
- & Achilleas S. Frangakis
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Letter |
In situ structural analysis of the human nuclear pore complex
The most comprehensive architectural model to date of the nuclear pore complex reveals previously unknown local interactions, and a role for nucleoporin 358 in Y-complex oligomerization.
- Alexander von Appen
- , Jan Kosinski
- & Martin Beck
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Letter |
Eye-like ocelloids are built from different endosymbiotically acquired components
Dinoflagellate eye-like ocelloids are built from pre-existing organelles of disparate origin, including a cornea-like layer made of mitochondria and a retinal body made of anastomosing plastids.
- Gregory S. Gavelis
- , Shiho Hayakawa
- & Brian S. Leander
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Letter |
Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles
Structures of actin-like ParM filaments at near-atomic resolution and their arrangements into doublets reveal how subunits and filaments come together to segregate low-copy-number plasmid R1 in Escherichia coli, producing the simplest known mitotic machinery.
- Tanmay A. M. Bharat
- , Garib N. Murshudov
- & Jan Löwe
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Letter |
Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution
Cryo-electron tomography and subtomogram averaging methods reveal the structure of the capsid lattice within intact heterogeneous immature HIV-1 particles.
- Florian K. M. Schur
- , Wim J. H. Hagen
- & John A. G. Briggs
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Letter |
Icosahedral bacteriophage ΦX174 forms a tail for DNA transport during infection
Here, the atomic structure of a virally encoded, cell-wall-spanning, DNA-translocating conduit from bacteriophage ΦX174 is described.
- Lei Sun
- , Lindsey N. Young
- & Bentley A. Fane
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Letter |
Visualizing virus assembly intermediates inside marine cyanobacteria
This study reports the first application of Zernike phase contrast (ZPC) electron cryo-tomography to examine cellular processes without the need for labelling or sectioning; the technique is used to visualize the maturation of the cyanophage Syn5 inside its host cell, identifying subcellular compartments and five distinct Syn5 assembly intermediates.
- Wei Dai
- , Caroline Fu
- & Wah Chiu
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Article |
Type VI secretion requires a dynamic contractile phage tail-like structure
Microscopy reveals the dynamics of the type VI secretion system of Vibrio cholerae and its structural and functional resemblance to the contractile tail sheath of bacteriophages.
- M. Basler
- , M. Pilhofer
- & J. J. Mekalanos
