Cytological techniques articles within Nature

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  • News & Views |

    Irrespective of an organism's size, the proportional sizes of its parts remain constant. An experimental model reveals size-dependent adjustment of segment formation and gene-expression oscillations in vertebrates. See Letter p.101

    • Naama Barkai
    •  & Ben-Zion Shilo

  • Letter |

    An ex vivo primary culture assay is developed that recapitulates mouse embryonic mesodermal patterning and segment formation; using this approach, it is shown that oscillating gene activity is central to maintain stable proportions during development.

    • Volker M. Lauschke
    • , Charisios D. Tsiairis
    •  & Alexander Aulehla

  • News |

    The master builders that underlie tumour growth may inform treatment strategies.

    • Monya Baker

  • Letter |

    Using genetic lineage tracing, tumour cells are traced in vivo in an unperturbed solid tumour; in a carcinogen-induced papilloma mouse model, cells in these benign lesions are found to mirror the clonal hierarchy organization of normal tissue.

    • Gregory Driessens
    • , Benjamin Beck
    •  & Cédric Blanpain

  • News & Views |

    A method commonly employed to study replicative ageing in yeast is laborious and slow. The use of miniaturized culture chambers opens the door for automated molecular analyses of individual cells during ageing.

    • Michael Polymenis
    •  & Brian K. Kennedy

  • Article |

    Argonaute proteins are an essential part of the guide-RNA–protein complex that carries out RNA-induced gene silencing; structure–function studies of the yeast complex reveal conserved features of the eukaryotic complex, which underlie formation of the catalytically active conformation.

    • Kotaro Nakanishi
    • , David E. Weinberg
    •  & Dinshaw J. Patel

  • News & Views |

    Developing organs adapt dynamically to meet the changing needs of a growing organism. A study in zebrafish reveals surprising patterns of muscle growth that reshape the heart as it matures. See Article p.479

    • Deborah Yelon

  • News & Views |

    Drugs used to treat African sleeping sickness are outdated, and how they enter cells and exert biological effects is poorly understood. A genome-wide study using RNA interference provides valuable insight. See Letter p.232

    • Alan H. Fairlamb

  • Technology Feature |

    To understand biological heterogeneity, researchers are learning how to profile the molecular contents of individual cells.

    • Charlotte Schubert

  • Article |

    Organogenesis relies on the orchestration of many cellular interactions to create the collective cell behaviours that progressively shape developing tissues. Using a three-dimensional embryonic stem cell culture system, this study successfully generated neural retinal tissues that formed a fully stratified neural retinal structure with all the major components located in their proper spatial location as seen during optic-cup development in vivo. This approach might have important implications for stem cell therapy for retinal repair.

    • Mototsugu Eiraku
    • , Nozomu Takata
    •  & Yoshiki Sasai

  • Letter |

    Reproducing the complex process of spermatogenesis in vitro might lead to the development of new diagnostic and therapeutic techniques for male infertility. This study establishes in vitro organ culture conditions that can support complete spermatogenesis in mice. The in-vitro-derived spermatids and sperm produced healthy and fertile mice, and testis tissue fragments used as a starting material for in vitro spermatogenesis could be cryopreserved for months and then resumed full spermatogenesis in vitro.

    • Takuya Sato
    • , Kumiko Katagiri
    •  & Takehiko Ogawa

  • Books & Arts |

    Anthony King explores a Dublin exhibition that exposes the controversies of synthetic biology.

    • Anthony King

  • Brief Communications Arising |

    • Sabine Conrad
    • , Markus Renninger
    •  & Thomas Skutella

  • Letter |

    One of two papers showing the generation of haematopoietic stem cells (HSCs) from the ventral wall of the dorsal aorta in live zebrafish embryos. Here, combined fluorescent reporter transgenes, confocal time-lapse microscopy and flow cytometry identify and isolate the stepwise intermediates as aortic haemogenic endothelium transitions to nascent HSCs. HSCs generated from this haemogenic endothelium are the lineal founders of virtually all of the adult haematopoietic system.

    • Julien Y. Bertrand
    • , Neil C. Chi
    •  & David Traver

  • Letter |

    High mutation rates in the influenza A virus facilitate the generation of viral escape mutants, rendering vaccines and drugs potentially ineffective, but targeting host cell determinants could prevent viral escape. Here, 287 human host cell genes influencing influenza A virus replication are found using a genome-wide RNA interference screen. An independent assay is then used to investigate overlap between genes necessary for different viral strains.

    • Alexander Karlas
    • , Nikolaus Machuy
    •  & Thomas F. Meyer

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