Nanocrystallography articles within Nature

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• Article
  14 February 2024 | Open Access

  Influence of pump laser fluence on ultrafast myoglobin structural dynamics

  Ultrafast time-resolved serial femtosecond crystallography is used to investigate a photodissociation reaction in a protein, revealing the strong impact of the pump laser fluence on the structural changes  and the reaction mechanism.

  • Thomas R. M. Barends
  • , Alexander Gorel
  •  & Ilme Schlichting

• Article
  03 May 2023 | Open Access

  Structural evidence for intermediates during O₂ formation in photosystem II

  Using serial femtosecond X-ray cystallography, we provide structural insights into the final reaction step of Kok’s photosynthetic water oxidation cycle, specifically the S₃→[S₄]→S₀ transition where O₂ is formed.

  • Asmit Bhowmick
  • , Rana Hussein
  •  & Vittal K. Yachandra

• Article | 03 March 2021

  Structural insights into the inhibition of glycine reuptake

  Serial synchrotron crystallography reveals the structure of the human glycine transporter GlyT1, showing how a state-specific inhibitor exerts its effects, and potentially informing the design of new GlyT1 inhibitors to treat a range of disorders of the central nervous system.

  • Azadeh Shahsavar
  • , Peter Stohler
  •  & Poul Nissen

• Article | 02 December 2020

  Ultrafast structural changes within a photosynthetic reaction centre

  Time-resolved serial femtosecond crystallography is used to reveal the structural changes that stabilize the charge-separation steps of electron-transfer reactions in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds.

  • Robert Dods
  • , Petra Båth
  •  & Richard Neutze

• Article | 08 July 2020

  Structures of fungal and plant acetohydroxyacid synthases

  Structures of the acetohydroxyacid synthase complexes of Saccharomyces cerevisiae and Arabidopsis thaliana provide insights into the biosynthesis of and feedback inhibition by branched-chain amino acids.

  • Thierry Lonhienne
  • , Yu Shang Low
  •  & Luke W. Guddat

• Letter | 07 November 2018

  Structures of the intermediates of Kok’s photosynthetic water oxidation clock

  Crystallography and spectroscopy are used to solve high-resolution structures of the intermediates of Kok’s S-state clock in photosystem II.

  • Jan Kern
  • , Ruchira Chatterjee
  •  & Vittal K. Yachandra

• Letter | 17 May 2017

  Human GLP-1 receptor transmembrane domain structure in complex with allosteric modulators

  Crystal structures of the human GLP-1 receptor in complex with two negative allosteric modulators reveal a common binding pocket, and, together with mutagenesis and modelling studies, further our understanding of the receptor activation mechanism.Author: Please check the wording of the following statement, which will appear online only.

  • Gaojie Song
  • , Dehua Yang
  •  & Raymond C. Stevens

• Letter | 20 February 2017

  Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL

  A new approach, time-resolved serial femtosecond crystallography, is used to view the intermediate states of a photosystem complex following illumination, shedding light on proton transfer and O=O bond formation.

  • Michihiro Suga
  • , Fusamichi Akita
  •  & Jian-Ren Shen

• Letter | 21 November 2016

  Structure of photosystem II and substrate binding at room temperature

  The structures of three intermediate states of photosystem II, which is crucial for photosynthesis, have been solved at room temperature, shedding new light on this process.

  • Iris D. Young
  • , Mohamed Ibrahim
  •  & Junko Yano

• Letter | 14 November 2016

  Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography

  Femtosecond XFEL crystallography is used to identify dynamic changes in the adenine riboswitch aptamer domain, with at least four states identified in real time, two in the apo form before binding and two with the ligand bound.

  • J. R. Stagno
  • , Y. Liu
  •  & Y.-X. Wang

• Article | 28 September 2016

  De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure

  The structure of the bacterial toxin BinAB, which is used to combat mosquito-borne diseases, reveals pH-sensitive switches and carbohydrate-binding modules that may contribute to the larvicidal function of the toxin.

  • Jacques-Philippe Colletier
  • , Michael R. Sawaya
  •  & David S. Eisenberg

• Letter | 10 February 2016

  Macromolecular diffractive imaging using imperfect crystals

  Crystal lattice disorder, which gives rise to a continuous diffraction pattern, is exploited to determine the structure of the integral membrane protein complex photosystem II to a higher resolution than could be achieved using Bragg diffraction alone.

  • Kartik Ayyer
  • , Oleksandr M. Yefanov
  •  & Henry N. Chapman

• Letter | 11 May 2014

  Ribosomal oxygenases are structurally conserved from prokaryotes to humans

  Crystal structures of human and prokaryotic ribosomal oxygenases reported here, with and without their ribosomal protein substrates, support their assignments as hydroxylases, and provide insights into the evolution of the JmjC-domain-containing hydroxylases and demethylases.

  • Rasheduzzaman Chowdhury
  • , Rok Sekirnik
  •  & Christopher J. Schofield