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| Open AccessDesign and testing of a humanized porcine donor for xenotransplantation
Using kidneys from a genetically engineered porcine donor transplanted into a cynomolgus monkey model, the design, creation and long-term function of kidney grafts supporting life are explored.
- Ranjith P. Anand
- , Jacob V. Layer
- & Wenning Qin
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Article
| Open AccessThe PTPN2/PTPN1 inhibitor ABBV-CLS-484 unleashes potent anti-tumour immunity
An orally bioavailable small-molecule active-site inhibitor of the phosphatases PTPN2 and PTPN1, ABBV-CLS-484, demonstrates immunotherapeutic efficacy in mouse models of cancer resistant to PD-1 blockade.
- Christina K. Baumgartner
- , Hakimeh Ebrahimi-Nik
- & Robert T. Manguso
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Article |
Lung adenocarcinoma promotion by air pollutants
Combination of epidemiology, preclinical models and ultradeep DNA profiling of clinical cohorts unpicks the inflammatory mechanism by which air pollution promotes lung cancer
- William Hill
- , Emilia L. Lim
- & Charles Swanton
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Article
| Open AccessFXR inhibition may protect from SARS-CoV-2 infection by reducing ACE2
FXR regulates the levels of ACE2 in tissues of the respiratory and gastrointestinal systems that are affected by COVID-19, and inhibiting FXR with ursodeoxycholic acid downregulates ACE2 and reduces susceptibility to SARS-CoV-2 infection.
- Teresa Brevini
- , Mailis Maes
- & Fotios Sampaziotis
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Article
| Open AccessPD-1-cis IL-2R agonism yields better effectors from stem-like CD8+ T cells
Binding of the PD1-IL2v immunocytokine to PD-1 and IL-2Rβγ on the same cell leads to an alternative differentiation of stem-like CD8+ T cells into better effectors rather than exhausted T cells in models of both chronic infection and cancer.
- Laura Codarri Deak
- , Valeria Nicolini
- & Pablo Umaña
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Article |
PHGDH heterogeneity potentiates cancer cell dissemination and metastasis
PHDGH heterogeneity in primary tumours could be a sign of tumour aggressiveness.
- Matteo Rossi
- , Patricia Altea-Manzano
- & Sarah-Maria Fendt
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Article |
Clonal fitness inferred from time-series modelling of single-cell cancer genomes
Whole-genome sequencing of human cancer cells in patient-derived mouse xenograft models indicates a key role for TP53 in determining the fitness landscape of polyclonal cancer cell populations.
- Sohrab Salehi
- , Farhia Kabeer
- & Sohrab P. Shah
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Article |
Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmia
Heart atria produce a large pool of calcitonin (previously well-recognized as a thyroid-secreted hormone with roles in calcium and bone metabolism) that in the heart acts as a paracrine signal controlling atrial fibrosis and fibrillation.
- Lucia M. Moreira
- , Abhijit Takawale
- & Svetlana Reilly
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Article |
Hydroxychloroquine use against SARS-CoV-2 infection in non-human primates
Hydroxychloroquine did not confer protection against SARS-CoV-2 infection or reduce the viral load after infection in macaques; these findings do not support the use of hydroxychloroquine as an antiviral drug treatment of COVID-19 in humans.
- Pauline Maisonnasse
- , Jérémie Guedj
- & Roger Le Grand
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Article |
International evaluation of an AI system for breast cancer screening
An artificial intelligence (AI) system performs as well as or better than radiologists at detecting breast cancer from mammograms, and using a combination of AI and human inputs could help to improve screening efficiency.
- Scott Mayer McKinney
- , Marcin Sieniek
- & Shravya Shetty
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Letter |
Small-molecule ion channels increase host defences in cystic fibrosis airway epithelia
Amphotericin B forms nonselective transmembrane ion channels, and restores host defences of cystic fibrosis airway epithelia independently of the regulatory function of cystic fibrosis transmembrane conductance.
- Katrina A. Muraglia
- , Rajeev S. Chorghade
- & Martin D. Burke
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Letter |
Evidence for an alternative fatty acid desaturation pathway increasing cancer plasticity
In several human and mouse cancer cell lines and carcinomas, a sapienate biosynthesis pathway underpins metabolic plasticity by allowing these cells to bypass stearoyl-CoA desaturase-dependent fatty acid desaturation.
- Kim Vriens
- , Stefan Christen
- & Sarah-Maria Fendt
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Letter |
Consistent success in life-supporting porcine cardiac xenotransplantation
α1,3-galactosyltransferase-knockout pig hearts that express human CD46 and human thrombomodulin require non-ischaemic preservation with continuous perfusion and post-transplantation growth control to ensure long-term orthotopic function of the xenograft in baboons.
- Matthias Längin
- , Tanja Mayr
- & Jan-Michael Abicht
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Article |
A homing system targets therapeutic T cells to brain cancer
Therapeutic T cells bearing ligands engineered to optimize adhesion and transmigration through the blood–brain barrier can be targeted to brain tumours.
- Heba Samaha
- , Antonella Pignata
- & Nabil Ahmed
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Letter |
TGFβ drives immune evasion in genetically reconstituted colon cancer metastasis
A combination of TGFβ inhibition and checkpoint-inhibition therapy provokes a potent cytotoxic response against metastatic tumours derived from colorectal cancers in mice.
- Daniele V. F. Tauriello
- , Sergio Palomo-Ponce
- & Eduard Batlle
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Letter |
Targeting renal cell carcinoma with a HIF-2 antagonist
The HIF-2 antagonist PT2399 is tested in mice bearing tumourgrafts derived from human renal cell cancers to demonstrate its efficacy, identify markers of sensitivity and characterize its effects.
- Wenfang Chen
- , Haley Hill
- & James Brugarolas
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Letter |
Nanoparticle biointerfacing by platelet membrane cloaking
The authors report a new biomimetic nanodelivery platform in which polymeric nanoparticles enclosed in the plasma membrane of human platelets are used for disease-relevant targeting, and the therapeutic potential of the concept is demonstrated in animal models of coronary restenosis and systemic bacterial infection.
- Che-Ming J. Hu
- , Ronnie H. Fang
- & Liangfang Zhang
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Article |
Loss of oncogenic Notch1 with resistance to a PI3K inhibitor in T-cell leukaemia
Mutations that dysregulate Notch1 and Ras/PI3K signalling are common in T-cell acute lymphoblastic leukaemia; here, treatment with a PI3K inhibitor is shown to induce drug resistance that is associated with downregulation of activated Notch1 signalling, suggesting that inhibition of both Notch1 and PI3K could promote drug resistance.
- Monique Dail
- , Jason Wong
- & Kevin Shannon
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Letter |
Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts
Regeneration of the heart muscle after myocardial infarction with cardiomyocytes derived from human embryonic stem cells is demonstrated in non-human primates, with the grafts showing evidence of electromechanical coupling, although they were also associated with non-fatal arrhythmias.
- James J. H. Chong
- , Xiulan Yang
- & Charles E. Murry
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Perspective
| Open AccessA call for transparent reporting to optimize the predictive value of preclinical research
Deficiencies in methods reporting in animal experimentation lead to difficulties in reproducing experiments; the authors propose a set of reporting standards to improve scientific communication and study design.
- Story C. Landis
- , Susan G. Amara
- & Shai D. Silberberg
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Correspondence |
Keep 'reproducibility' in context
- Brad Picha
- , Matthew Thompson
- & Thomas M. Vondriska
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News |
Drug candidates derailed in case of mistaken identity
PARP inhibitor that wasn't highlights widespread flaws in preclinical studies.
- Heidi Ledford
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Letter |
Clinical efficacy of a RAF inhibitor needs broad target blockade in BRAF-mutant melanoma
PLX4032 is a selective inhibitor of the B-RAF protein that has shown promising results in an early clinical trial in melanoma patients with an activating mutation in B-RAF. Now the structure and function of this inhibitor are described. Translational data from a phase I trial show that clinical efficacy requires a substantial degree of inhibition of the ERK pathway downstream of B-RAF. The data also show that BRAF-mutant melanomas are highly dependent on B-RAF activity.
- Gideon Bollag
- , Peter Hirth
- & Keith Nolop
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News |
Protein jab mends broken bones
Injecting mice with Wnt proteins speeds up healing.
- Janelle Weaver
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Article |
N-myristoyltransferase inhibitors as new leads to treat sleeping sickness
African sleeping sickness, caused by Trypanosoma brucei species, is responsible for some 30,000 human deaths each year. Available treatments are limited by poor efficacy and safety profiles. However, a new molecular target for potential treatments has now been identified. The protein target is T. brucei N-myristoyltransferase. In further experiments, lead compounds have been discovered that inhibit this protein, kill trypanosomes in vitro and in vivo, and can cure trypanosomiasis in mice.
- Julie A. Frearson
- , Stephen Brand
- & Paul G. Wyatt