Featured
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Letter |
Dynamics of ribosome scanning and recycling revealed by translation complex profiling
A translation complex sequencing approach has been developed enabling intermediates of all mRNA-associated processes of translation to be isolated and localized across the transcriptome; the results support longstanding models of initiation and termination and offer new mechanistic insights.
- Stuart K. Archer
- , Nikolay E. Shirokikh
- & Thomas Preiss
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Article |
Quantitative reactivity profiling predicts functional cysteines in proteomes
Cysteine is the most intrinsically nucleophilic amino acid in proteins, but the absence of a consensus sequence that defines functional cysteines in proteins has hindered their discovery and characterization. Here, a proteomics method to quantitatively profile the intrinsic reactivity of cysteine residues directly in native biological systems is described. Hyper-reactive cysteines were identified in several proteins of uncharacterized function, including a residue conserved across eukaryotes that is shown to be required for yeast viability and involved in iron–sulphur protein biogenesis.
- Eranthie Weerapana
- , Chu Wang
- & Benjamin F. Cravatt
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Letter |
Aneuploidy confers quantitative proteome changes and phenotypic variation in budding yeast
Profiling of a large set of aneuploid yeast strains grown under a variety of conditions demonstrates that aneuploidy can affect both the transcriptome and the proteome and can generate significant phenotypic variation that could lead to fitness gains.
- Norman Pavelka
- , Giulia Rancati
- & Rong Li
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News |
China pushes for the proteome
Strategy to build a complete catalogue of human proteins could put the country in a leading position.
- David Cyranoski
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Review Article |
Expansion of the eukaryotic proteome by alternative splicing
- Timothy W. Nilsen
- & Brenton R. Graveley