Stem-cell research

Article | 27 March 2024

Depleting myeloid-biased haematopoietic stem cells rejuvenates aged immunity

Antibody-mediated depletion of myeloid-biased haematopoietic stem cells in aged mice restores characteristic features of a more youthful immune system.

Jason B. Ross
, Lara M. Myers
& Irving L. Weissman

Article
15 November 2023 | Open Access

CD201⁺ fascia progenitors choreograph injury repair

Spatiotemporal regulation of wound healing in mice and humans occurs via retinoic acid and hypoxia signalling, which regulate the differentiation of CD201⁺ fibroblast progenitors into proinflammatory and myofibroblast states.

Donovan Correa-Gallegos
, Haifeng Ye
& Yuval Rinkevich

Article | 20 September 2023

A multi-stem cell basis for craniosynostosis and calvarial mineralization

The calvarial stem cell niche is populated by a cathepsin K-expressing cell lineage and a newly identified discoidin domain-containing receptor 2-expressing lineage, both of which are required for proper calvarial mineralization.

Seoyeon Bok
, Alisha R. Yallowitz
& Matthew B. Greenblatt

Article | 20 April 2022

Generation of 3D lacrimal gland organoids from human pluripotent stem cells

Organoids originating from human multipotent ocular surface epithelial stem cells are similar to native lacrimal glands and undergo functional maturation when transplanted adjacent to the eyes of recipient rats, developing lumina and producing tear-film proteins.

Ryuhei Hayashi
, Toru Okubo
& Kohji Nishida

Article | 24 February 2021

An organoid-based organ-repurposing approach to treat short bowel syndrome

In a rat model of short bowel syndrome, transplantation of small intestinal organoids into the colon partially restores intestinal function and improves survival—a proof of principle that organoid transplantation might have therapeutic benefit.

Shinya Sugimoto
, Eiji Kobayashi
& Toshiro Sato

Article | 23 December 2020

Nociceptive nerves regulate haematopoietic stem cell mobilization

Stimulation of pain-sensing neurons, which can be achieved in mice by the ingestion of capsaicin, promotes the migration of haematopoietic stem cells from the bone marrow into the blood.

Xin Gao
, Dachuan Zhang
& Paul S. Frenette

Article | 18 December 2019

Somatic inflammatory gene mutations in human ulcerative colitis epithelium

Whole-exome sequencing of colon organoids derived from patients with ulcerative colitis identifies somatic mutations in components of the IL-17 signalling pathway, which may confer a growth advantage to cells under inflammatory conditions.

Kosaku Nanki
, Masayuki Fujii
& Toshiro Sato

Article | 27 November 2019

MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment

MLLT3 is identified as a crucial regulator of the self-renewal of human haematopoietic stem cells, and helps to maintain an active chromatin state in haematopoietic stem-cell regulatory genes during culture.

Vincenzo Calvanese
, Andrew T. Nguyen
& Hanna K. A. Mikkola

Letter | 14 November 2018

Linking a cell-division gene and a suicide gene to define and improve cell therapy safety

Introduction of a suicide gene together with a linked cell-division gene to generate a safe-cell system enables the selective elimination of proliferating cells after cell transplantation in mouse models of cell therapy.

Qin Liang
, Claudio Monetti
& Andras Nagy

Letter | 04 April 2018

Advanced maturation of human cardiac tissue grown from pluripotent stem cells

A tissue culture system that provides an increasing intensity of electromechanical stimulation over time enables an in vitro model of cardiac tissue derived from human induced pluripotent stem cells to develop many of the characteristics of adult cardiac tissue.

Kacey Ronaldson-Bouchard
, Stephen P. Ma
& Gordana Vunjak-Novakovic

Article | 08 November 2017

Regeneration of the entire human epidermis using transgenic stem cells

Autologous transgenic epidermal stem cell cultures are used to reconstitute almost the entire epidermis of a patient with severe junctional epidermolysis bullosa.

Tobias Hirsch
, Tobias Rothoeft
& Michele De Luca

Letter | 27 September 2017

A reversible haploid mouse embryonic stem cell biobank resource for functional genomics

The Haplobank contains over 100,000 individually reversibly mutagenized, barcoded, mouse embryonic cell lines; proof-of-principle experiments were used to search for genes that are required for rhinovirus infection and angiogenesis using forward and reverse genetic screens, respectively.

Ulrich Elling
, Reiner A. Wimmer
& Josef M. Penninger

Letter | 20 September 2017

Comparative glycoproteomics of stem cells identifies new players in ricin toxicity

A novel quantitative approach to identify intact glycopeptides from comparative proteomic data sets, allowing inference of complex glycan structures and direct mapping of them to sites within the associated proteins at the proteome scale.

Johannes Stadlmann
, Jasmin Taubenschmid
& Josef M. Penninger

Article | 17 May 2017

Conversion of adult endothelium to immunocompetent haematopoietic stem cells

The authors reprogram in vitro endothelial cells from adult mice into engraftable haematopoietic stem cells that display single-cell and multilineage properties, are capable of long-term self-renewal and can reconstitute T cell adaptive immune function.

Raphael Lis
, Charles C. Karrasch
& Shahin Rafii

Letter | 10 October 2016

Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts

Allogenic induced pluripotent stem cell-derived cardiomyocytes transplanted directly into infarcted cynomolgus monkey hearts show electrical coupling with host cardiomyocytes improve cardiac contractile function after mild immunosuppression.

Yuji Shiba
, Toshihito Gomibuchi
& Uichi Ikeda

Letter | 07 October 2015

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

The kidney arises from two types of progenitors; here, the signalling conditions that induce the production of collecting ducts and functional nephrons from human pluripotent stem cells are determined, and organoids that recapitulate the functional regionalization of the kidney are produced.

Minoru Takasato
, Pei X. Er
& Melissa H. Little

Spotlight | 11 June 2014

Spotlight on Stem Cell Research

Letter | 30 April 2014

Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts

Regeneration of the heart muscle after myocardial infarction with cardiomyocytes derived from human embryonic stem cells is demonstrated in non-human primates, with the grafts showing evidence of electromechanical coupling, although they were also associated with non-fatal arrhythmias.

James J. H. Chong
, Xiulan Yang
& Charles E. Murry

Letter | 02 March 2014

Direct measurement of local oxygen concentration in the bone marrow of live animals

Here, using two-photon phosphorescence lifetime microscopy, the local oxygen tension in the bone marrow of live mice is found to be quite low, with spatiotemporal variations depending on the blood vessel type, distance to the endosteum, and changes in cellularity after stress.

Joel A. Spencer
, Francesca Ferraro
& Charles P. Lin

Article | 09 October 2013

Arteriolar niches maintain haematopoietic stem cell quiescence

Immunofluorescence imaging and computational modelling are used to study the spatial distribution of different cell types within the haematopoietic stem cell (HSC) niche; findings show that quiescent HSCs associate specifically with small arterioles that are preferentially found in the endosteal bone marrow and are essential in maintaining this quiescence.

Yuya Kunisaki
, Ingmar Bruns
& Paul S. Frenette

Spotlight | 05 June 2013

Spotlight on Stem Cell Research

Inside View | 30 January 2013

Inside View: Bettencourt Schueller Foundation

News | 18 December 2012

Features of the year

The best of our longer reads as selected by our editors.

Research Highlights | 17 October 2012

Stem cells wrap neurons

News | 16 October 2012

Stem-cell fraud hits febrile field

After heart-treatment claims collapse, researchers caution against a rush to the clinic.

David Cyranoski

News | 08 October 2012

Cell rewind wins medicine Nobel

Researchers awarded prestigious prize for their work on reprogramming mature cells to a pluripotent state.

Alison Abbott

Correspondence | 05 September 2012

Tighten up Japan's stem-cell practices

Hiroshi Ikegaya

Editorial | 20 June 2012

Renewed vigour

Stem-cell researchers must engage with politicians to keep their work alive in Europe.

News | 20 June 2012

Rudimentary liver grown in vitro

Japanese scientists coax pluripotent cells to form functional liver tissue.

David Cyranoski

Spotlight | 30 May 2012

Spotlight on Stem-Cell Research

News | 01 May 2012

South Korea steps up stem-cell work

Regenerative medicine gets cash boost but stricter regulations are needed to ensure safety.

Soo Bin Park

Research Highlights | 25 April 2012

Patient-specific heart cells

News | 11 April 2012

China’s stem-cell rules go unheeded

Health ministry’s attempt at regulation has had little effect.

David Cyranoski

News | 26 March 2012

Vatican calls off stem-cell conference

Embryonic-stem-cell researchers question sudden cancellation of annual meeting.

Ewen Callaway

Q&A | 14 March 2012

Turning point: Sean Bendall

Following the ‘road not taken’ philosophy, stem-cell specialist earns award and start-up money.

Virginia Gewin

Outlook | 29 February 2012

Q&A: Stemming the tide of misinformation

As director of outreach experiences at the Morgridge Institute for Research in Madison, Wisconsin, Nirupama Shevde spreads the word about stem cells. Nature Outlook finds out what she has to say.