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| Open AccessUbiquitin ligation to F-box protein targets by SCF–RBR E3–E3 super-assembly
Cryo-electron microscopy of neddylated SCF-family ligases interacting with the RBR-type E3 ligase ARIH1 reveals the steps through which E3–E3 super-assemblies ubiquitylate a diverse set of substrates presented on F-box proteins.
- Daniel Horn-Ghetko
- , David T. Krist
- & Brenda A. Schulman
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Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity
Biochemical, structural and functional studies on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) papain-like protease PLpro reveal that it regulates host antiviral responses by preferentially cleaving the ubiquitin-like interferon-stimulated gene 15 protein (ISG15) and identify this protease as a potential therapeutic target for coronavirus disease 2019 (COVID-19).
- Donghyuk Shin
- , Rukmini Mukherjee
- & Ivan Dikic
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Structure of the DDB1–CRBN E3 ubiquitin ligase in complex with thalidomide
The crystal structures of thalidomide and its derivatives bound to the E3 ligase subcomplex DDB1–CRBN are shown; these drugs are found to have dual functions, interfering with the binding of certain cellular substrates to the E3 ligase but promoting the binding of others, thereby modulating the degradation of cellular proteins.
- Eric S. Fischer
- , Kerstin Böhm
- & Nicolas H. Thomä
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53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark
This study shows that 53BP1 recruitment to sites of DNA damage involves dual recognition of H4K20me2 and H2AK15 histone ubiquitination; the ubiquitin mark and the surrounding epitope on H2A are read by a region of 53BP1 designated the ubiquitination-dependent recruitment motif.
- Amélie Fradet-Turcotte
- , Marella D. Canny
- & Daniel Durocher
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Enhancement of proteasome activity by a small-molecule inhibitor of USP14
In the ubiquitin–proteasome system, substrates destined for destruction are modified with ubiquitin chains and then degraded by the proteasome. These authors reveal a regulatory mechanism in which proteasomal activity is modulated by the length of ubiquitin chains in human cells. They find that deubiquitinating enzyme USP14 can inhibit the degradation of ubiquitin-conjugated substrates by trimming ubiquitin chains, and that stimulation of proteasome activity may be used to reduce the levels of toxic proteins in cells.
- Byung-Hoon Lee
- , Min Jae Lee
- & Daniel Finley