Article
|
Open Access
Featured
-
-
Article |
Clonally expanded B cells in multiple sclerosis bind EBV EBNA1 and GlialCAM
The identification of high-affinity molecular mimicry between the Epstein–Barr virus (EBV) transcription factor EBNA1 and the CNS protein GlialCAM provides a mechanistic link between multiple sclerosis and EBV.
- Tobias V. Lanz
- , R. Camille Brewer
- & William H. Robinson
-
Article |
Mucosal or systemic microbiota exposures shape the B cell repertoire
A mouse model of systemic versus mucosal exposure to microbial taxa reveals that the former provokes a flexible B cell response with a diverse immunoglobulin repertoire, whereas the latter generates a more-restricted response.
- Hai Li
- , Julien P. Limenitakis
- & Andrew J. Macpherson
-
Article |
CTCF orchestrates long-range cohesin-driven V(D)J recombinational scanning
Using an auxin-inducible approach, the authors show that downmodulation of CTCF activity promotes cohesin-driven RAG endonuclease scanning, and thus V(D)J recombination, across the Igh locus.
- Zhaoqing Ba
- , Jiangman Lou
- & Frederick W. Alt
-
Article |
Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion
Pax5 regulates contraction of the immunoglobulin heavy chain (Igh) locus—an essential step in V(D)J recombination—by promoting chromatin loop extrusion via repression of Wapl expression.
- Louisa Hill
- , Anja Ebert
- & Meinrad Busslinger
-
Article |
Structures of a RAG-like transposase during cut-and-paste transposition
Analysis of multiple structures of the Helicoverpa zea DNA transposase Transib, determined by X-ray crystallography and cryo-electron microscopy, reveals the detailed pathway of the transposition reaction and sheds light on the evolution of the RAG recombinase.
- Chang Liu
- , Yang Yang
- & David G. Schatz
-
Letter |
Analysis of the B cell receptor repertoire in six immune-mediated diseases
An analysis of the B cell receptor repertoire in six immune-mediated diseases reveals that there are substantial differences in clonality, isotype use, class switching and use of the IGHV genes between diseases.
- R. J. M. Bashford-Rogers
- , L. Bergamaschi
- & K. G. C. Smith
-
Letter |
The fundamental role of chromatin loop extrusion in physiological V(D)J recombination
V(D)J recombination in B cells involves cohesin-mediated extrusion of chromatin loops to present DNA targets for cleavage and joining.
- Yu Zhang
- , Xuefei Zhang
- & Frederick W. Alt
-
Article |
Transposon molecular domestication and the evolution of the RAG recombinase
Identification of the changes that converted a transposase to a recombinase sheds light on the evolution of the vertebrate adaptive immune system.
- Yuhang Zhang
- , Tat Cheung Cheng
- & David G. Schatz
-
Letter |
Commonality despite exceptional diversity in the baseline human antibody repertoire
A genetic study of the baseline human antibody repertoire, based on the circulating B cell populations of ten subjects, reveals universally shared antibody clonotypes within repertoires that are largely unique to the individual.
- Bryan Briney
- , Anne Inderbitzin
- & Dennis R. Burton
-
Letter |
Quantifiable predictive features define epitope-specific T cell receptor repertoires
The authors characterize epitope-specific T cell repertoires, identify shared and recognizable features of TCRs, and develop tools to classify antigen specificity on the basis of sequence analysis.
- Pradyot Dash
- , Andrew J. Fiore-Gartland
- & Paul G. Thomas
-
Article |
Crystal structure of the V(D)J recombinase RAG1–RAG2
The crystal structure of the RAG1–RAG2 heterotetramer forms a Y-shaped structure, with each arm containing a RAG1–RAG2 heterodimer; the overall structure is reminiscent of hairpin-forming transposases, attesting to its evolutionary history as a specialized form of a transposition activity.
- Min-Sung Kim
- , Mikalai Lapkouski
- & Martin Gellert
-
Article |
CTCF-binding elements mediate control of V(D)J recombination
- Chunguang Guo
- , Hye Suk Yoon
- & Frederick W. Alt
-
Letter |
ATM damage response and XLF repair factor are functionally redundant in joining DNA breaks
Although loss of XLF, a classical non-homologous DNA end-joining (NHEJ) repair factor, shows strong effects in non-lymphoid cells, in lymphoid cells its absence has only modest effects on V(D)J recombination. This study now shows that in lymphoid cells, two other repair factors — ATM kinase and histone protein H2AX — have functional redundancy with XLF. Thus, mice deficient in both ATM and XLF have compromised conventional NHEJ, although alternative end-joining is retained. The results hint that the redundant function in end-joining that XLF has with both ATM and H2AX may have to do with an ATM role in chromatin accessibility.
- Shan Zha
- , Chunguang Guo
- & Frederick W. Alt
Functional antibodies exhibit light chain coherence