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Phenotypic screening system using three-dimensional (3D) culture models for natural product screening

The Journal of Antibiotics volume 74, pages 660–666 (2021)Cite this article

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Abstract

Recent progress in three-dimensional (3D) cell culture systems has attracted much attention in the fields of basic life science and drug development. Newly established methods include 3D co-culture, spheroid culture, and organoid culture; these methods enable more human tissue-like culture and have largely replaced traditional two-dimensional (2D) monolayer culture. By combining 3D culture methods with high-content imaging analysis, it is possible to obtain diverse and convincing data even during initial screening (which requires rapid and easy operating procedures). Until recently, 3D culture methods were considered expensive, time-consuming, complex, and unstable. However, by exploiting the self-assembling nature of cells and adding several technical improvements, we have developed several phenotypic screenings aimed at discovering anticancer compounds.

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Acknowledgements

This work was supported by Technology Research Association for Next Generation Natural Products Chemistry.

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Authors and Affiliations

  1. National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan

    Hikaru Suenaga & Kazuo Shin-ya

  2. Technology Research Association for Next Generation Natural Products Chemistry, Tokyo, Japan

    Noritaka Kagaya & Kazuo Shin-ya

  3. Institute of Microbial Chemistry (BIKAKEN), Numazu, Microbial Chemistry Research Foundation, Shizuoka, Japan

    Manabu Kawada

  4. Institute of Microbial Chemistry (BIKAKEN), Microbial Chemistry Research Foundation, Tokyo, Japan

    Manabu Kawada & Daisuke Tatsuda

  5. Department of Organoid Medicine, Keio University School of Medicine, Tokyo, Japan

    Toshiro Sato

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  1. Hikaru Suenaga
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Contributions

KS designed and managed the study; HS prepared screening samples; NK constructed assay systems and practiced screening; MK and DT established fluorescent labeled Panc-1 cells; TS established CSC cells; HS, NK, and KS wrote the manuscript.

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Correspondence to Kazuo Shin-ya.

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Suenaga, H., Kagaya, N., Kawada, M. et al. Phenotypic screening system using three-dimensional (3D) culture models for natural product screening. J Antibiot 74, 660–666 (2021). https://doi.org/10.1038/s41429-021-00457-8

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