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Protaetiibacter mangrovi sp. nov., isolated from mangrove soil

The Journal of Antibiotics volume 76, pages 532–539 (2023)Cite this article

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Abstract

A novel Gram-stain-positive, aerobic, non-flagellated and rod-shaped actinobacterium, designated 10F1B-8-1T, was isolated from mangrove soil sampled at Futian Mangrove Nature Reserve, China. The isolate was able to grow at 10–40 °C (optimum 30–32 °C), at pH 6–8 (optimum 7) and in the presence of 0–6% (w/v) NaCl (optimum 0%). Strain 10F1B-8-1T shared the highest 16S rRNA gene sequence similarity to Protaetiibacter larvae NBRC 113051T (98.3%), followed by Protaetiibacter intestinalis NBRC 113050T (98.2%). Phylogenetic trees based on 16S rRNA gene sequences and the core proteomes exhibited that strain 10F1B-8-1T formed a new phyletic line in the clade of genus Protaetiibacter, indicating that this strain belonged to the genus Protaetiibacter. Strain 10F1B-8-1T showed low average nucleotide identity (<84%) and digital DNA-DNA hybridization values (<27%) with closely related taxa, suggesting that strain 10F1B-8-1T was a hitherto undescribed species of the genus Protaetiibacter. Strain 10F1B-8-1T contained D-2,4-diaminobutyric acid as the diagnostic diamino acid, and the peptidoglycan type was characterized as type B2β. The major fatty acids were iso-C16:0, anteiso-C15:0 and anteiso-C17:0. The major menaquinones were MK-13 and MK-14. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and five unidentified lipids. Notably, the ethyl acetate extracts of strain 10F1B-8-1T showed effective antibacterial activity against Bacillus subtilis CPCC 100029 and Escherichia coli △tolC. According to the polyphasic data, strain 10F1B-8-1T should be classified as a novel species of the genus Protaetiibacter, for which the name Protaetiibacter mangrovi sp. nov. is proposed, with the type strain 10F1B-8-1T (=JCM 33142T = CPCC 205428T).

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Acknowledgements

This research was supported by the Beijing Natural Science Foundation (BJNSF, Grant number 5224038 and 7222256), the National Natural Science Foundation of China (NSFC, Grant number 32200001), and Foreign Youth Talent Program QN2022194001L funded by Ministry of Science and Technology of the People’s Republic of China.

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Author notes

  1. These authors contributed equally: Feina Li, Xiaomeng Hao.

Authors and Affiliations

  1. Laboratory of Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Center for Children’s Health, Beijing, 100045, China

    Feina Li, Huiwen Zheng & Chen Shen

  2. Department of Microbial Chemistry, Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China

    Feina Li, Xiaomeng Hao, Qinpei Lu, Shaowei Liu & Chenghang Sun

  3. Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, China

    Li Tuo

  4. Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50275, Indonesia

    Mada Triandala Sibero

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  1. Feina Li
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Li, F., Hao, X., Lu, Q. et al. Protaetiibacter mangrovi sp. nov., isolated from mangrove soil. J Antibiot 76, 532–539 (2023). https://doi.org/10.1038/s41429-023-00627-w

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