Abstract
A new formanilide dimer, fraxinin (1), and three known formanilides (2‒4) were isolated from the culture broth of Perenniporia fraxinea using silica gel and Sephadex LH-20 column chromatographies, medium-pressure liquid chromatography (MPLC), and preparative HPLC. The structures of these compounds were determined by spectroscopic methods, such as NMR and mass analysis, and by comparison of the spectra with previously reported data. The free radical scavenging activities of the isolated compounds were assessed using 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Compounds 1‒3 exhibited ABTS radical scavenging activity with IC50 values in the range of 57.2–250.2 μM. Compounds 2 and 4 marginally reduced disease incidence of powdery mildew with a control value of 42% at 1.0 mg ml−1 in cucumber leaf disk assay.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Szczepkowski A. Perenniporia fraxinea (fungi, polyporales). Pol Bot J. 2004;49:73–7.
Wen CN, Hu DB, Bai X, Wang F, Li ZH, Feng T, et al. Chemical constituents from fruiting bodies of Basidiomycete Perenniporia subacida. Fitoterapia. 2016;109:179–84.
Hirotani M, Ino C, Furuya T, Shirot M. Perenniporiol derivatives six triterpenoids from the cultured mycelia of Perenniporia ochroleuca. Phytochemistry. 1984;23:1129–34.
Wu LS, Hu CL, Han T, Zheng CJ, Ma XQ, Rahman K, et al. Cytotoxic metabolites from Perenniporia tephropora, an endophytic fungus from Taxus chinensis var. mairei. Appl Microbiol Biotechnol. 2013;97:305–15.
Kim JY, Woo EE, Ha LS, Ki DW, Lee IK, Yun BS. Three new meroterpenoids from culture broth of Perenniporia medulla-panis and their antioxidant activities. J Antibiot. 2019;72:625–8.
Feng Y, Wang L, Niu S, Li L, Si Y, Liu X, et al. Naphthalenones from a Perenniporia sp. inhabiting the larva of a phytophagous weevil, Euops chinesis. J Nat Prod. 2012;75:1339–45.
Kim DH, Kim MJ, Kim DW, Kim GY, Kim JK, Gebru YA, et al. Changes of phytochemical components (urushiols, polyphenols, gallotannins) and antioxidant capacity during Fomitella fraxinea–mediated fermentation of Toxicodendron vernicifluum bark. Molecules. 2019;24:683.
Tanaka N, Kitamura A, Mizushina Y, Sugawara F, Sakaguchi K. Fomitellic acids, triterpenoid inhibitors of eukaryotic DNA polymerases from a basidiomycete, Fomitella fraxinea. J Nat Prod. 1998;61:193–7.
Cho SM, Yun BS, Yoo ID, Koshino H. Structure of fomitellan A, a mannofucogalactan from the fruiting bodies of Fomitella fraxinea. Bioorg Med Chem Lett. 2011;21:204–6.
Yoshikawa K, Koso K, Shimomura M, Tanaka M, Yamamoto H, Imagawa H, et al. Yellow pigments, fomitellanols A and B, and drimane sesquiterpenoids, cryptoporic acids p and q, from Fomitella fraxinea and their inhibitory activity against COX and 5-LO. Molecules. 2013;18:4181–91.
Park SS, Lee JS, Bae KG, Yu KH, Han HC, Min TJ. Antioxidative activity and structural analysis of the steroid compound from Fomitella fraxinea. Kor J Mycol. 2001;29:67–71.
Yin J, Zhang J, Cai C, Deng G-J, Gong H. Catalyst-free transamidation of aromatic amines with formamide derivatives and tertiary amides with aliphatic amines. Org Lett. 2019;21:387–92.
Corbett MD, Corbett BR. Reaction of nitroso aromatics with glyoxylic acid. A new path to hydroxamic acids. J Org Chem. 1980;45:2834–9.
Ashalatha K, Raja Shantha Reddy A, Raveesha H. Evaluation of phytochemical compounds and antimicrobial activities of Hemidesmus indicus. Asian J Pharm Clin Res. 2019;12:102–6.
Kim JY, Ki DW, Lee YJ, Ha LS, Woo EE, Lee IK et al. Consoramides A–C, New Zwitterionic Alkaloids from the Fungus Irpex consors. Mycobiology 2021;49:434–7.
Lee IK, Kim SE, Yeom JH, Ki DW, Lee MS, Song JG, et al. Daldinan A, a novel isoindolinone antioxidant from the ascomycete Daldinia concentrica. J Antibiot. 2012;65:95–7.
Ki DW, Kim DW, Hwang BS, Lee SW, Seok SJ, Lee IK, et al. New antioxidant sesquiterpenes from a culture broth of Coprinus echinosporus. J Antibiot. 2015;68:351–3.
Li YH, Windham MT, Trigiano RN, Fare DC, Spiers JM, Copes WE. Evaluation for resistance to powdery mildew in Cornus species and hybrids using a leaf disk assay. J Environ Hort. 2007;25:131–3.
Acknowledgements
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Crop Viruses and Pests Response Industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (grant number 321103-3) and also supported by BK21 FOUR Program by Jeonbuk National University Research Grant. Authors thank Ms. Ji-Young Oh, Center for University-wide Research Facilities (CURF) at Jeonbuk National University, for performing the NMR measurements.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
About this article
Cite this article
Choi, DC., Ki, DW., Kim, YH. et al. Formanilides from the culture broth of Perenniporia fraxinea. J Antibiot 76, 731–734 (2023). https://doi.org/10.1038/s41429-023-00665-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41429-023-00665-4