Abstract
A new antifungal compound, named N-demethyltyroscherin (1), was discovered from the static fungal cultured material of Scedosporium apiospermum FKJ-0499 isolated from a deep-sea sediment sample together with a known compound, tyroscherin (2). The structure of 1 was elucidated as a new analog of 2 by MS and NMR analyses. The absolute configuration of 1 was determined by chemical derivatization. Both compounds showed potent in vitro antifungal activity against clinically isolated Candida auris strains, with MIC values ranging from 0.0625 to 4 µg ml–1.
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Acknowledgements
We are grateful to Distinguished Emeritus Professor Satoshi Ōmura (Kitasato University) for his helpful support and valuable guidance and suggestions. We thank Dr. Kenichiro Nagai, Ms. Reiko Seki, and Ms. Noriko Sato (School of Pharmacy, Kitasato University) for various instrumental analyses. We also thank Dr. Hidetaka Nomaki for his great help in collecting the deep-sea sample. The authors would like to thank the Centers for Disease Control and Prevention and the National Institute of Infectious Diseases for providing the AR Isolate bank strains. This research was partially supported by Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under Grant Number JP21am0101096 (Phase I) and JP22ama121035 (Phase II). This work was supported by the JSPS KAKENHI Grant (21K05293 and 23H04887).
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Azami, H., Watanabe, Y., Sakai, K. et al. Antifungal profile against Candida auris clinical isolates of tyroscherin and its new analog produced by the deep-sea-derived fungal strain Scedosporium apiospermum FKJ-0499. J Antibiot 77, 156–162 (2024). https://doi.org/10.1038/s41429-023-00696-x
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DOI: https://doi.org/10.1038/s41429-023-00696-x
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