Abstract
cAMP is a universal second messenger regulated by various upstream pathways including Ca2+ and G-protein-coupled receptors (GPCRs). To decipher in vivo cAMP dynamics, we rationally designed cAMPinG1, a sensitive genetically encoded green cAMP indicator that outperformed its predecessors in both dynamic range and cAMP affinity. Two-photon cAMPinG1 imaging detected cAMP transients in the somata and dendritic spines of neurons in the mouse visual cortex on the order of tens of seconds. In addition, multicolor imaging with a sensitive red Ca2+ indicator RCaMP3 allowed simultaneous measurement of population patterns in Ca2+ and cAMP in hundreds of neurons. We found Ca2+-related cAMP responses that represented specific information, such as direction selectivity in vision and locomotion, as well as GPCR-related cAMP responses. Overall, our multicolor suite will facilitate analysis of the interaction between the Ca2+, GPCR and cAMP signaling at single-cell resolution both in vitro and in vivo.
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Main
cAMP is a universal second messenger in a variety of cell types and organisms. Individual cell types express various adenylate cyclases (ACs) and phosphodiesterases that synthesize and degrade cAMP, respectively. The upstream regulators of the ACs include GPCRs and another central second messenger, Ca2+, through Ca2+-dependent ACs1. cAMP is regulated by these multiple upstream signaling pathways and modulates diverse cellular functions through cAMP-dependent kinases, channels and transcription factors. Therefore, technologies to visualize the spatiotemporal dynamics of cAMP in vivo are crucial for biological research in various organs or species.
Since cAMP was visualized in 1991, more than 50 cAMP indicators have been developed2,3, and recently, several cAMP indicators have been used in vivo4,5,6,7,8. The circularly permuted green fluorescent protein (cpGFP)-type cAMP indicators have been intensively developed more recently due to their large dynamic range6,7,9,10,11. However, the use of these indicators has been limited due to their cAMP affinity (>1 μM), which is lower than that of endogenous cAMP-dependent kinases and channels (typically in the hundreds of nanomolar range)12,13.
Here, we present cAMPinG1, a green cAMP indicator with a high dynamic range and more than 4-fold higher cAMP affinity than the existing green cAMP indicators. We also introduce RCaMP3, an improved red calcium indicator. Dual-color imaging of RCaMP3 and cAMPinG1 revealed dynamic interaction and information flow between Ca2+, cAMP and GPCRs in vivo. In addition, we demonstrated the application of cAMPinG1 imaging in cultured cells for studying GPCR biology.
Results
Rational engineering of a sensitive cAMP sensor
To develop a high-affinity cAMP sensor, we chose a mammalian protein kinase A regulatory subunit (PKA-R) as the cAMP-sensing domain. PKA-Rs are widely distributed and functional in mammalian neurons even when tagged by GFP and overexpressed14 and have been well characterized in terms of their biochemical, evolutionary and structural properties13,15,16,17. We used the cAMP-binding domain A of mammalian PKA-R type 1α (PKA-R1α) because of its affinity for cAMP (around 150 nM)18, which was within the range of the affinity of cAMP-binding domains in multiple PKAs and cyclic nucleotide-gated ion channels7,8 (Extended Data Fig. 1a). We inserted cpGFP into the β4–β5 loop of PKA-R1α for the following reasons: (1) it is close to cAMP in the cAMP-bound three-dimensional structure, (2) it is exposed on the surface, and (3) it is structurally flexible, as demonstrated by the crystal structure analysis and evolutionarily conserved sequences15,19, to avoid possible structural perturbations20 (Fig. 1a and Extended Data Fig. 1b,c). We also removed the N-terminal PKA-R1α region to avoid interaction with endogenous proteins (Extended Data Fig. 1a). Instead, we fused the RSET sequence to the N terminus of the sensor to promote stable expression19. To improve dynamic range (ΔF/F), we then screened over 250 mutants with mutations on the putative interface between the cpGFP and cAMP-binding domain, including two linkers and residues in cpGFP close to the interface. The variant with the largest ΔF/F was named cAMP indicator green 1 (cAMPinG1; Fig. 1b). Excitation spectra of cAMPinG1 indicated green fluorescence intensity of cAMPinG1 increased by 1,000% upon binding to cAMP with blue light (488 nm), while the green fluorescence intensity decreased by 61% with violet light (405 nm), resulting in a 2,700% ratio change with a combination of blue and violet excitation in HEK293T cell lysate (Fig. 1c). In contrast, G-Flamp1 showed less fluorescence decrease with violet excitation7 (Extended Data Fig. 2a).
We next developed a cAMP-insensitive indicator (cAMPinG1mut (inactive mutant)) by introducing the p.Arg211Glu (in the numbering of mouse PKA-R1α) mutation to block cAMP binding (Fig. 1d). To detect cAMP changes selectively in the cytoplasm, we added a self-cleaving peptide (F2A) to the C termini of cAMPinG1 (named nuclear-excluded cAMPinG1 (cAMPinG1-NE)21 (Fig. 1d). Furthermore, to avoid contamination of somatic neuropil fluorescence signals, we linked ribosomal subunit protein (RPL10) to the C terminus of cAMPinG1 as soma-targeting (named cAMPinG1-ST)22 (Fig. 1d).
In vitro characterization of cAMPinG1
Side-by-side comparison revealed that cAMPinG1 had the largest ΔF/F at 490 nm of excitation and fluorescence intensity in a cAMP-saturated state in HEK293T cell lysate than the existing cAMP sensors, Flamindo2, gCarvi and G-Flamp1 (refs. 7,9,23; Fig. 1e, Extended Data Fig. 2a and Supplementary Table 1). The ΔF/F of cAMPinG1 at 450 nm of excitation was less than that of G-Flamp1, indicating that cAMPinG1 was tuned to the 470–490 nm of excitation, frequently used for green fluorescence imaging (Fig. 1e). cAMPinG1mut did not respond to cAMP, confirming that the fluorescence change of cAMPinG1 depended on cAMP binding. The Kd value of cAMPinG1 was 181 nM, less than a quarter of those of Flamindo2, gCarvi and G-Flamp1(Fig. 1f,g). Furthermore, we validated the linear relation of cAMP concentration and cAMPinG1 fluorescence intensity, low affinity to cGMP (Kd = 12.2 µM), in vitro properties of cAMPinG1-NE, pH sensitivity, in vitro kinetics and undetectable binding to PKA-C (Extended Data Fig. 2c–j and Supplementary Table 2).
As expected by excitation spectra (Fig. 1c), time-lapse imaging for HEK293T cells showed an increase in green fluorescence of cAMPinG1 with blue (488 nm) excitation and decreased with violet (405 nm) excitation in response to the application of forskolin (FSK), an activator of ACs (Fig. 1h–j). While cAMPinG1 demonstrated a high ΔF/F (~400%) for intensiometric measurement with 488 nm of excitation, ratiometric measurement had an even higher ΔF/F (~800%). Moreover, cAMPinG1 showed a fluorescence lifetime increase from 2.461 to 2.644 in response to forskolin (Extended Data Fig. 2k).
In acute brain slices, the elevation of cAMPinG1-NE fluorescence by forskolin/isobutylmethylxanthine (IBMX) administration was observed with single-cell resolution (Extended Data Fig. 3a–d). Moreover, the cAMPinG1-NE response to local puff application of dopamine in the striatum and bath application of norepinephrine in the cortex was also observed, which was significantly larger than that of G-Flamp1 (P = 1.2 × 10−3 and 4.6 × 10−6, unpaired two-tailed t-test; Extended Data Fig. 3e–l). We did not observe any abnormalities in the excitability and synaptic transmission of neurons expressing cAMPinG1-NE (Extended Data Fig. 3m–o).
In vivo imaging of cAMP dynamics with subcellular resolution
We introduced cAMP sensors into pyramidal neurons in layer 2/3 (L2/3) of the mouse primary visual cortex (V1) by in utero electroporation (Fig. 2a). We applied an aversive airpuff stimulus in the awake condition to induce an elevation of norepinephrine and cAMP in the cortex5. Somatic cAMPinG1 imaging visualized transient cAMP signals induced by a 20-s airpuff with single-cell resolution (Fig. 2b–e). We also confirmed that cAMPinG1mut did not respond to the airpuff stimulus. (Fig. 2d,e). In addition, the ΔF/F of cAMPinG1 was significantly larger than that of G-Flamp1 (P = 9.2 × 10−6 between cAMPinG1 and G-Flamp1, unpaired two-tailed t-test; Fig. 2d,e). The half-decay time of the cAMP transients was around 20 s, in contrast to the Ca2+ transients on the order of hundreds of milliseconds (Fig. 2f).
Then, we imaged cAMPinG1-NE in dendritic spines and shafts in vivo under lightly anesthetized conditions (Fig. 2g). We observed sensory-evoked cAMP transients in both dendritic spines and shafts (Fig. 2h,i). Thus, cAMPinG1 allows in vivo cAMP imaging, which requires advanced sensitivity of the indicators such as spine imaging.
Engineering improved red Ca2+ indicator RCaMP3
Ca2+ signaling is one of the upstream modulators of cAMP signaling through Ca2+-dependent ACs. To facilitate the combinational use of the green cAMP indicator in vivo, we developed a red Ca2+ indicator. A series of red Ca2+ indicators has been developed based on R-GECO1 (refs. 21,24,25,26,27,28; Extended Data Fig. 4a). We introduced the following mutations into jRGECO1a; p.Met128Arg/p.Ile131Val/p.Val139Leu/p.Phe188Leu/p.Asn231Ser/p.Ser234Thr (in R-GECO1 numbering) from R-GECO1.2 to blue-shift the excitation spectra, and p.Glu217Asp. from sRGECO to generate a large dynamic range. In addition, we used a self-cleaving peptide (F2A) instead of the nuclear export signal of jRGECO1a21. We termed this hybrid indicator RCaMP3 (Fig. 3a).
In vitro characterization revealed that, while RCaMP3 exhibits similar pH sensitivity and photostability to jRGECO1a and XCaMP-R, it shows a larger dynamic range and more blue-shifted excitation spectum24,26 (Fig. 3b and Extended Data Fig. 4b–l). The blue-shifted excitation spectrum of RCaMP3 is well suited for two-photon imaging because the excitation peaks of jRGECO1a and XCaMP-R24,26 are longer than 1,040 nm, the fixed wavelength of the commonly available dual-wavelength lasers. Indeed, RCaMP3 showed higher fluorescence than jRGECO1a and XCaMP-R in the Ca2+-saturated state excited at 1,040 nm (Fig. 3c).
In acute brain slices, RCaMP3 had larger ΔF/F to single action potentials than jRGECO1a with comparable rise and decay kinetics (Fig. 3d–h). In addition, we performed loose-seal cell-attached electrical recording and two-photon Ca2+ imaging simultaneously in vivo (Fig. 3i). The ΔF/F of RCaMP3 correlated with the number of action potentials and was consistently higher than that of jRGECO1a across all spike numbers, despite their comparable kinetics. (Fig. 3k–n). We note that the aggregation of RCaMP3 was comparable with that of jRGECO1a (Supplementary Fig. 1).
To demonstrate the sensitivity of RCaMP3, we used fast-scanning high optical invariant two-photon microscopy (FASHIO-2PM)29. We performed RCaMP3 imaging in a large field of view (3.0 × 3.0 mm2) including the primary somatosensory cortices (Fig. 3o and Supplementary Video 1). Somatic Ca2+ transients of several thousands of L5 neurons were simultaneously monitored with single-cell resolution (Fig. 3p). In addition, somatic Ca2+ transients of L2/3 neurons and dendritic Ca2+ transients of L5 neurons could be monitored when imaged in L2/3 (Extended Data Fig. 5).
Dual-color Ca2+ and cAMP imaging during forced running
To visualize Ca2+ and cAMP dynamics with single-cell resolution in vivo, we coexpressed RCaMP3 and cAMPinG1-ST in L2/3 neurons of the V1 by adeno-associated virus (AAV) injection (Fig. 4a). Using a piezo objective scanner, we performed multiple z-plane imaging of head-fixed awake mice (Fig. 4b). cAMPinG1-ST and RCaMP3 were excited with 940 nm and 1,040 nm, respectively. cAMPinG1-ST fluorescence was localized to somata due to the soma-targeting signal RPL10 (ref. 22) (Supplementary Fig. 2). The fluorescence intensity of cAMPinG1 was considerably higher than that of G-Flamp1 when coexpressing Ca2+ and cAMP sensors (Supplementary Fig. 3). Here, we simultaneously detected Ca2+ and cAMP signals of more than 400 neurons in L2/3 of a mouse (Fig. 4c,d and Supplementary Video 2). During the imaging, we used a forced running task to increase norepinephrine, cAMP and PKA activities in the cortex7,8,30. The majority of neurons showed cAMP increase during running (Fig. 4d,e). The fluorescence changes in cAMPinG1-ST resulted from cAMP binding to the sensor and were significantly larger than those of G-Flamp1 (P = 4.6 × 10−2 between cAMPinG1-ST and G-Flamp1, unpaired two-tailed t-test; Extended Data Fig. 6a–d). In addition, this global cAMP increase was less cell specific and dependent on norepinephrine8,31 and β-adrenoceptors (Extended Data Fig. 6e–g). Furthermore, consistent with a previous report32, around 25% of cells showed calcium transients during running, as detected by RCaMP3 (Fig. 4d,e). These motion-related cells had larger cAMP transients than the other non-motion-related cells (Fig. 4f–h). This additional cAMP elevation with Ca2+ responses may be attributed to Ca2+-dependent ACs.
To demonstrate the application of cAMPinG1 and RCaMP3 for cell types other than neurons, we expressed RCaMP3 and cAMPinG1-NE in astrocytes in L2/3 of the V1 (ref. 33). Forced running induced Ca2+ increase followed by cAMP increase in astrocytes (Extended Data Fig. 6h–j).
Dual-color Ca2+ and cAMP imaging during visual stimulation
To further investigate the relation between Ca2+ and cAMP in vivo, a drifting grating stimulus of eight directions was applied to induce cell-specific Ca2+ transients in L2/3 neurons of the V1 (Fig. 5a). RCaMP3 showed direction-selective Ca2+ transients in response to 4 s of drifting gratings, consistent with previous studies26,34 (Fig. 5b–d and Extended Data Fig. 7a,b). Interestingly, cAMPinG1-ST also showed direction-selective cAMP transients preceded by Ca2+ transients, which were selective to the same direction and positively correlated with Ca2+ (Fig. 5b–d and Extended Data Fig. 7a,b).
Then, we conducted an experiment with repetitive moving gratings of the same direction (8 s, three times). Neurons responding to the direction in Ca2+ levels exhibited a cAMP increase, as observed above (Fig. 5e–g). We observed that the cAMPinG1-ST fluorescence began to decrease in the majority of Ca2+-responsive and Ca2+-unresponsive cells in the middle of visual stimuli. This global cAMP decrease lasted beyond the end of the stimulus and cAMPinG1 fluorescence was lower than the baseline observed before the stimulation (Fig. 5e–g). These fluorescence changes in cAMPinG1-ST resulted from cAMP binding to the sensor and were significantly higher than those of G-Flamp1 (P = 7.2 × 10−4 between cAMPinG1-ST and G-Flamp1, unpaired two-tailed t-test after removing outliers with Smirnov–Grubbs’ test; Extended Data Fig. 7c,d). We did not find any contamination of the fluorescence signal from RCaMP3 in the green channel (Extended Data Fig. 7e). Overall, two-photon imaging of RCaMP3 and cAMPinG1-ST revealed bidirectional cAMP change and strong correlation between Ca2+ and cAMP with single-cell resolution in vivo.
Action potentials induce somatic cAMP elevation
To determine whether the calcium-related cAMP increase detected above was related to action potentials, we applied single-cell optogenetic stimulation and cAMP imaging in vivo. To achieve spatially precise optical stimulation to the soma of a neuron, we sparsely expressed cAMPinG1-NE and soma-targeted ChRmine in L2/3 neurons of the V1 by utilizing the Cre/loxP recombination system35 (Fig. 5h,i). We observed an increase in cAMP levels in response to two-photon single-cell stimulation at 1,040 nm (Fig. 5j,k), indicating that action potentials are sufficient to induce somatic cAMP elevation.
Dual-color fiber photometry for Ca2+ and cAMP imaging
We next evaluated the use of cAMPinG1 and RCaMP3 in single-photon fiber photometry in deep brain areas. In line with previous studies7,8,36, we observed cAMP elevation during the running period following the increase in Ca2+ levels in the dorsal striatum (Extended Data Fig. 8). These fluorescence signals arose from cAMP binding and were more intense than those of G-Flamp1.
cAMPinG1 imaging for GPCR biology and drug screening
To demonstrate the utility of cAMPinG1 in cultured cells, we performed ratiometric imaging by measuring the 488 nm excitation and 405 nm excitation ratio (488 ex/405 ex) at a single timepoint after forskolin or mock application. The 488 ex/405 ex ratio in each cell was higher in forskolin-stimulated cells than in non-stimulated cells (Extended Data Fig. 9a). Furthermore, the ratio change of cAMPinG1 was significantly larger than those of G-Flamp1 and cAMPFIRE-L (P < 1.0 × 10−13 between cAMPinG1 and G-Flamp1, P < 1.0 × 10−13 between cAMPinG1 and cAMPFIRE-L, Tukey’s post hoc test following one-way analysis of variance (ANOVA); Extended Data Fig. 9a–c).
To apply this single-cell, single-timepoint cAMPinG1 imaging approach for GPCR biology and pharmacology, we expressed a dopamine receptor D1 (DRD1), a Gs-coupled GPCR with constitutive activity in the cAMP pathway, in the cAMPinG1 stable cell line (Fig. 6a and Extended Data Fig. 9c,d). We fused a self-cleaving 2A peptide (P2A) and RFP to DRD1 to monitor the expression level of DRD1 in each cell. cAMPinG1 imaging revealed a positive correlation between DRD1 expression and cAMP level37, which diminished with dopamine (Fig. 6a,b). Consistent with previous studies reporting high constitutive activity of GPCR GPR52 (refs. 37,38), we also observed higher cAMP levels of GPCR GPR52 than those of Gs-coupled β2-adrenoceptor (ADRB2) and DRD1 (Fig. 6e). These results suggest that the cAMPinG1 imaging can be available to compare the constitutive activity of cAMP-related GPCRs. The cAMPinG1 imaging also allowed quantifying the agonist activity of a Gi-coupled dopamine receptor D2 (DRD2) as well as that of DRD1 (Fig. 6c,d). The cAMPinG1 imaging also detected inverse agonist activity of clozapine for a Gs-coupled serotonin receptor HTR6 as well as the agonist activity of serotonin (Fig. 6f). Furthermore, the cAMPinG1 imaging visualized that multiple GPCRs in the same field of view specifically responded to their respective agonists (Extended Data Fig. 10a–d). These results suggest that single-cell, single-timepoint cAMPinG1 imaging has the potential for multiplex high-throughput drug screenings.
Discussion
In this study, we reported cAMPinG1 and RCaMP3, a multicolor suite for cAMP and Ca2+ imaging, and showed applications that required advanced sensitivity of these indicators. Furthermore, we addressed an important biological question: the relation between Ca2+ and cAMP signaling in vivo.
Each existing cAMP sensor has some drawbacks for in vivo imaging7,8. While the basal cAMP concentration varies depending on cell types and experimental conditions, it is estimated to be at ~100 nM in neurons in vitro2,39. Therefore, the high affinity of cAMPinG1 (Kd = 181 nM) is well suited to capture these changes, although potential cAMP buffering effects should be considered. G-Flamp1 limited the number of cells that could be imaged and quantified cAMP dynamics in the mouse cortex7, possibly due to low cAMP affinity (Kd = ~1 μM) as well as low expression efficacy. Another cAMP sensor, cAMPFIRE8, exceeds the packaging capacity of the AAV vector due to the size of cDNA (approximately 4.5 kb), limiting its in vivo delivery methods. cAMPinG1 (about 1.7 kb) with high cAMP affinity and a large dynamic range overcome these issues. Further investigation with cAMPinG1 will shed light on in vivo dynamics and actual concentrations of cAMP in various neuronal and glial compartments, which are assumed to be modulated within microdomains40,41.
The global cAMP increase in the V1 during forced running depended on Gs-coupled β-adrenoceptors5,7,8,31. On the contrary, the mechanisms of the global cAMP decrease observed during and after stimulations in the cortex and dorsal striatum remain unknown. Some Gi-coupled GPCRs and phosphodiesterases activated by Ca2+ or kinases42 can be involved. The detection of these bidirectional cAMP changes indicates that the cAMP affinity of cAMPinG1 is suitable for in vivo cAMP imaging.
Simultaneous RCaMP3 and cAMPinG1 imaging visualized population dynamics of Ca2+ and cAMP in hundreds of cells in the mouse cortex, demonstrating a robust positive correlation between Ca2+ and cAMP. In vivo optogenetic experiments demonstrated that action potentials are sufficient to induce somatic cAMP transients. This suggests that the spike-induced cAMP increase can be mediated by voltage-dependent Ca2+ channels and Ca2+-dependent ACs43, while the other non-cell-autonomous mechanisms cannot be ruled out. Our results suggest the possibility that cAMP can encode specific information, such as direction selectivity in vision or locomotion encoded in action potentials and Ca2+ signaling. This cell-specific cAMP elevation cooperated or competed with global cAMP increase or decrease, respectively, leading to the formation of population patterns of cAMP. Notably, cAMP transients last for tens of seconds, much longer than the hundreds of milliseconds of Ca2+ transients. Therefore, information encoded in Ca2+ and GPCR signaling can be integrated and stored for a longer period through cAMP transients.
Single-cell, single-timepoint ratiometric cAMP imaging in cultured cells depended on the spectral property of cAMPinG1. This brief and robust technique will contribute to GPCR analysis, such as the systematic determination of cAMP-mediated downstream pathways of GPCRs to address some discrepancies between the results of receptor–G-protein ‘couplome’ obtained from different assays44, as well as high-throughput drug screening.
Methods
Animals
All experimental procedures were performed using protocols that were approved by the Institutional Animal Care and Use Committee at Kyoto University (Lif-K23008), University of Yamanashi (A1-20), RIKEN (W2022-2-012) and The University of Tokyo (P18-118). Wild-type mice (C57BL/6N, ICR) and A2A-Cre ((B6.FVB (Cg)-Tg (Adora2a-cre) KG139Gsat/Mmucd)) mice were group-housed and kept on a 12-h light–dark cycle with ad libitum food and water. The housing conditions were controlled at room temperature of approximately 22–24 °C and a relative humidity of 40–60%. Wild-type mice used in this study were purchased from Japan SLC. Experiments were performed using both male and female sex between 0 and 20 weeks of age.
Cell lines
HEK293T cells were obtained from the American Type Culture Collection (CRL-11268). Cells were cultured in DMEM (08458-16, Nacalai) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), 50 units per ml penicillin and 50 μg ml−1 streptomycin (26252-94, Nacalai) at 37 °C and 5% CO2 in a humidified atmosphere. Escherichia coli (E. Coli) DH5α and DH10B, and Stbl3 cells were obtained from Toyobo (DNA-9303), Invitrogen (18297010) and Invitrogen (C737303), respectively. Bacteria were incubated in Lysogeny Broth (LB) medium supplemented with antibiotics at 37 °C.
Plasmids
To develop cAMP sensors, PKA-R1α (amino acids 108–186 and 190–381) was obtained from a mouse cDNA library, and cpGFP and RSET domain were subcloned from GCaMP6f (Addgene plasmid, 52924). The F2A sequence was from XCaMP-R24.The RPL10 domain was from GCaMP6m-RPL10a22. G-Flamp1 was synthesized (GENEWIZ). To develop red Ca2+ sensors, the cpRFP domain was synthesized (GENEWIZ), and RSET, RS20 and CaM domains were obtained from jRGECO1a26. For site-directed mutagenesis, plasmid libraries were made using the inverse PCR method with PrimeSTAR Max DNA polymerase (R045A, Clontech), In-Fusion HD Cloning Kit (639650, Clontech), and primers which included NNK codons, where K = G or T. For expression in E. coli, cAMP sensors were subcloned into a pBAD vector45. For optogenetic stimulation, ChRmine-mScarlet-Kv2.1 was synthesized (GENEWIZ). For expression in HEK293T cells, Ca2+ or cAMP sensors were subcloned into a plasmid encoding CAG promoter and woodchuck hepatitis virus posttranscriptional regulatory element (WPRE). For expression of GPCRs–P2A–mCherry in HEK293T cells, pTRE3G-HA signal-Flag–GPCRs–P2A–mCherry-reverse-PGK–TetOn3G was made of Tet-ON 3G inducible expression system (Clontech) and PRESTO-Tango GPCR Kit46.
In vitro fluorometry for cAMP sensor screening
The plasmids for bacterial expression of cAMP sensors were transformed into E. coli strain DH10B. E. coli cells were plated and cultured at 37 °C on an LB agar plate with ampicillin and 0.0004% arabinose. Each colony was used to inoculate 1.5 ml of LB liquid medium with ampicillin and 0.2% arabinose and grown at 37 °C overnight. After centrifugation, cells were resuspended in 150 µl suspension buffer (20 mM MOPS (pH 7.2), 100 mM KCl, 1 mM dithiothreitol (DTT), cOmplete EDTA-free protease inhibitor cocktail; 11836170001, Roche), sonicated at 4 °C, and centrifuged. The supernatant was collected. For fluorometry, the supernatant was diluted 20-fold with the suspension buffer. The diluted supernatant was applied to 96-well plates. cAMP (A2381, Tokyo Chemical Industry) was added to a final concentration of 300 µM for cAMP-saturated conditions. Fluorometric measurements were performed on a microplate reader (Spark, TECAN) at room temperature at 485 nm of excitation (bandwidth of 20 nm) and 535 nm of emission (bandwidth 20 nm).
In vitro cAMP fluorometry for HEK cell lysate
HEK293T cells were incubated on six-well plates. Next, 1 µg DNA was transfected using X-tremeGENE HP DNA Transfection Reagent (6366244001, Roche). Two days after the transfection, the cells were harvested into the 150 µl suspension buffer described above, sonicated at 4 °C, and centrifuged. The supernatant was collected. For cAMP-saturated conditions, the supernatant was diluted 20-fold with suspension buffer, and cAMP was added to a final concentration of 300 µM. Fluorometric measurements were performed at 485 nm or 450 nm of excitation (bandwidth 20 nm) and 545 nm of emission (bandwidth 20 nm). Excitation and emission spectra were taken at 555 nm and 460 nm, respectively. For measurement of cAMP affinity, suspension buffer with 0, 3, 10, 30, 100, 300, 1,000, 3,000, 10,000, 30,000, 100,000 and 300,000 nM cAMP was prepared. Then, the supernatant was diluted 40-fold with each suspension buffer. For measurement of cGMP affinity, suspension buffer with 0, 100, 300, 1,000, 3,000, 10,000, 30,000, 100,000, 300,000 and 1,000,000 nM cGMP (ab120805, Abcam) was prepared. Then, the supernatant was diluted 40-fold with each suspension buffer. For measurement of pKa, the supernatant was diluted 40-fold with pH buffer (20 mM citrate (for pH 5–6), 20 mM MOPS (for pH 6.5–10))25, and cAMP was added to a final concentration of 100 µM. pKa values were determined from the inflection point of a sigmoid fit to fluorescence versus pH.
In vitro Ca2+ fluorometry for HEK cell lysate
Cell incubation, transfection and collection were performed as described above. For Ca2+-saturated or Ca2+-free conditions, the supernatant was diluted 20-fold with the Ca2+-EGTA buffer (30 mM MOPS (pH 7.2), 100 mM KCl, 10 mM EGTA, 10 mM CaCl2 and 1 mM DTT) or EGTA buffer (30 mM MOPS (pH 7.2), 100 mM KCl, 10 mM EGTA and 1 mM DTT), respectively. Fluorometric measurements were performed at 560 nm of excitation (bandwidth of 20 nm) and 610 nm of emission (bandwidth of 20 nm). Excitation and emission spectra were taken at the emission of 635 nm and at the excitation of 520 nm, respectively. For measurement of Ca2+ affinity, the supernatant was diluted 40-fold with a series of solutions with free Ca2+ concentration ranges from 0 nM to 3,900 nM (ref. 27). For measurement of pKa, the supernatant was diluted 40-fold with pH buffer (20 mM citrate (for pH 5–6), 20 mM MOPS (for pH 6.5–10)) containing 2 mM CaCl2 or 2 mM EGTA25. pKa values were determined from the inflection point of a sigmoid fit to fluorescence versus pH.
In vitro kinetics analysis
The plasmids for bacterial expression of cAMPinG1, which included His-tag sequence, were transformed into E. coli strain DH10B. E. coli cells were plated and cultured at 37 °C on an agar plate with ampicillin. A colony was used to inoculate 200 ml of LB liquid medium with ampicillin and 0.2% arabinose and grown at 18 °C for 44 h. After centrifugation, cells were resuspended in 10 ml suspension buffer (25 mM Tris-HCl (pH 8.0), 300 mM NaCl, 1 mM DTT and cOmplete EDTA free (Sigma-Aldrich)), sonicated at 4 °C and centrifuged. The supernatant was collected.
Coverslips (no. 1, 15 mm diameter, Matsunami) were washed with 1% acetic acid solution for 6 h. Then, they were immersed in silanized solution containing 50% ethanol, 2% mercaptosilane and 1% acetic acid overnight, followed by drying at 143 °C for 1 h. They were immersed sequentially in reducing solution containing 50% ethanol, 2.5 mM ethylenediaminetetraacetic acid, 2 mM DTT and 100 mM phosphate buffer, pH = 7.0 for 1 h, 2.5 mg ml−1 maleimido-C3-NTA solution for 2 h, and 50 mM nickel sulfate solution for 1 h (ref. 9). The resultant nickel complex-coated coverslips were incubated in the supernatant including cAMPinG1 protein for 1 h at 4 °C. The cAMPinG1-binding coverslips were placed on the stage of an FVMPE-RS (Olympus) microscope equipped with a water-immersion ×25 objective lens (N.A.: 1.05, Olympus), a femtosecond laser (Insight DS+, Spectra-Physics) and GaAsP detector (Hamamatsu Photonics) with a 495–540-nm emission filter (Olympus). The laser was tuned at 920 nm. For measurement of binding kinetics (Extended Data Fig. 2g), cAMP was microperfused to the surface of the coverslips from 0 μM to 2 μM with a micropipette and a microinjector (BEX). Images (288 × 18 µm2, 128 × 8 pixels) were collected at 81.6 Hz. For measurement of dissociation kinetics (Extended Data Fig. 2h), cAMP was microperfused from 0.5 μM to 0 μM. Images (576 × 108 µm2, 256 × 48 pixels) were collected at 12.7 Hz.
cAMP imaging in HEK293T cells
For time-lapse imaging (Fig. 1h–j), HEK293T cells were incubated in 35-mm glass-bottom dishes. DNA encoding the sensors (1 µg) was transfected as described above. One day after the transfection, the culture medium was replaced with Tyrode’s solution (129 mM NaCl, 5 mM KCl, 30 mM glucose, 25 mM HEPES–NaOH, pH 7.4, 2 mM CaCl2, 2 mM MgCl2). Imaging for cAMPinG1 was performed using an LSM 880 confocal microscope (Carl Zeiss) with an air-immersion ×20 objective lens (N.A.: 0.80, Carl Zeiss). Then, 405-nm and 488-nm lasers were used for excitation in turns. Forskolin (16384-84, Nacalai) was added to a final concentration of 50 µM.
For single-timepoint imaging for GPCRs (Fig. 6), HEK293T cells were incubated in 96-well glass-bottom plates. Around 0.1 µg DNA encoding the sensors was transfected as described above. One day after the transfection, the culture medium was replaced with Tyrode’s solution. Imaging was performed using an LSM 880 confocal microscope with the ×20 objective lens. For tetracycline-dependent expression of GPCRs–P2A–mCherry, doxycycline was added to a final concentration of 100 ng ml−1 3 h before the imaging. Twenty minutes before the imaging, the culture medium was replaced with Tyrode’s solution with or without forskolin, dopamine (14212-71, Nacalai), clozapine (12059, Cayman), adrenocorticotropic hormone (AP3295, AdooQ,) or serotonin (18961-41, Nacalai). Lasers (405 nm and 488 nm) were used for ratiometric cAMP imaging, and 561-mn and 633-nm lasers were used for the visualization of GPCR-expressing cells.
For single-timepoint imaging for sensor comparison (Extended Data Fig. 9a–c), HEK293T cells were incubated in 96-well glass-bottom plates, transfected and imaged as described above. Imaging was performed to calculate the relative change (ΔR/R) in fluorescence ratio (R) in the absence or presence of 10 μM forskolin. For cAMPinG1 and G-Flamp1, R is the ratio of green fluorescence (491–553 nm of emission) with 488 nm of excitation to that with 405 nm of excitation. For cAMPFIRE-L, R is the ratio of cyan fluorescence (464–499 nm of emission) with 458 nm of excitation to yellow fluorescence (526–597 nm of emission) with 514 nm of excitation.
Binding assay in HEK293T cells
HEK293T cells were incubated in 35-mm glass-bottom dishes. DNA encoding the sensors (1 µg) was transfected as described above. One day after the transfection, the culture medium was replaced with Tyrode’s solution as described above. Imaging was performed using an LSM 880 confocal microscope with an oil-immersion ×40 objective lens (N.A.: 1.30, Carl Zeiss). Images (53.1 × 53.1 µm2, 512 × 512 pixels) were collected using 488-nm and 561-nm lasers.
Fluorescence lifetime measurement
HEK293T cells in 96-well glass-bottom plates were prepared as described above. Twenty minutes before the imaging, the culture medium was replaced with Tyrode’s solution with or without 10 µM forskolin. Imaging was performed using a TCS SP8 FALCON microscope (Leica) at a pulse frequency of 80 MHz with an air-immersion ×20 objective lens (N.A.: 0.75, Leica). Excitation was 488 nm by white-light laser and emission was 500–550 nm. Images (233 × 233 µm2, 256 × 256 pixels) were collected. The lifetime was analyzed using the LAS X FLIM/FCS software (Leica).
Two-photon Ca2+ imaging in HEK293T cells
HEK293T cells were incubated in 35-mm glass-bottom dishes. A mixture of 0.8 µg DNA encoding the red Ca2+ sensors and 0.2 µg DNA of pCMV-mCerulean was transfected as described above. One day after the transfection, the culture medium was replaced with Tyrode’s solution as described above. Thirty seconds after bath application of ionomycin (Cayman Chemical, 11932) to a final concentration of 5 µM, two-photon imaging was performed with an FVMPE-RS (Olympus) equipped with a water-immersion ×25 objective lens (N.A.: 1.05, Olympus), a femtosecond laser (Insight DS+, Spectra-Physics) and two GaAsP detectors (Hamamatsu Photonics) with 495–540-nm and 575–645-nm emission filters (Olympus). Images (339 × 339 µm2, 1,024 × 1,024 pixels, single optical section) were collected. The laser was tuned to 880 nm for mCerulean and 1,040 nm at the front aperture of the objective for the red Ca2+ sensors.
Photostability analysis
HEK293T cells were prepared in 96-well glass-bottom plates as described above. For one-photon bleaching measurements (Extended Data Fig. 4k), imaging was performed using an LSM 880 confocal microscope with a ×20 objective lens immersed with Tyrode’s solution. A 561-nm laser was used for excitation. Images (170 × 170 µm2, 512 × 512 pixels) were collected at 0.6 Hz. For two-photon bleaching measurement (Extended Data Fig. 4l), imaging was performed using a FVMPE-RS microscope with the ×20 objective lens in Tyrode’s solution. A 1,040-nm laser was used for excitation. Images (255 × 255 µm2, 256 × 256 pixels) were collected at 0.82 Hz.
Stable cell lines generation
Lentiviral particles were produced by transfection of the packaging plasmids with polyethylenimine into HEK293T cells47. Lentivirus-infected HEK293T cells were dissociated and isolated into multi-well plates. Single clones with bright fluorescence were picked, grown and stored at −80 °C. To establish a triple stable cell line (Extended Data Fig. 9), cAMPinG1 single stable cell line was infected with lentivirus encoding TRE3G–DRD1–EF1a–TetOn3G–P2A–mCherry–NLSx3 and TRE3G–MC3R–EF1a–TetOn3G–P2A–iRFP670 (ref. 48).
Cell proliferation assay
The HEK293T cells were seeded on six-well plates with 2 ml DMEM and 10% FBS or 1.5% FBS and incubated at 37 °C. The 1.5% FBS group was a positive control of slow cell proliferation. Twenty hours after the beginning of the culture, cells in half of the wells were harvested, and the number of cells was counted using a counting chamber as a timepoint of zero. Sixty hours after the beginning of the culture, cells in the other half of the wells were harvested and counted as a timepoint of 48 h.
In utero electroporation
ICR pregnant mice (Japan SLC) were anesthetized with an anesthetic mixture (0.075 mg ml−1 medetomidine hydrochloride, 0.40 mg ml−1 midazolam and 0.50 mg ml−1 butorphanol tartrate) and administered at 100 μl per 10 g of body weight intraperitoneally. Then, 2.0 µl of purified plasmid (1.0 µg µl−1 final concentration in each sensor) was injected into the right lateral ventricle of embryos at embryonic day 15. pCAG-green cAMP sensors-WPRE (cAMPinG1-NE, cAMPinG1mut-NE, G-Flamp1) and pCAG-RCaMP3-WPRE were delivered to induce the expression of cAMP and Ca2+ indicators in L2/3 pyramidal neurons in the V1. After soaking the uterine horn with warm saline (37 °C), each embryo’s head was carefully held between tweezers with platinum 5-mm disk electrodes (CUY650P5, Nepagene). Subsequently, five electrical pulses (45 V, 50-ms duration at 1 Hz) were delivered by an electroporator (NEPA21, Nepagene)49,50. Electroporated mice were used for cAMP and calcium imaging 4–10 weeks after birth.
AAV production and injection
Recombinant AAVs were produced using HEK293T cells and purified by AVB Sepharose51. The final titers were: AAV2/1-CAG-DIO-cAMPinG1-NE (5.0 × 1013 genome copies (GC) per ml), AAV2/1-CAG-DIO-cAMPinG1mut-NE (2.0 × 1013 GC per ml) and AAVPHP.eB-CaMKII-Cre (1.0 × 1012 GC per ml) for forskolin/IBMX bath application in acute brain slices (Extended Data Fig. 3a–d); AAV2/1-eSyn-cAMPinG1mut-NE (1.5 × 1013 GC per ml) and AAV2/1-CaMKII (0.3 kb)-loxFAS-H2B-mCherry-loxFAS (1.0 × 1013 GC per ml) for local dopamine application in acute brain slices (Extended Data Fig. 3e–h); AAV2/1-eSyn-cAMPinG1mut-NE (1.5 × 1013 GC per ml) and AAVPHP.eB-hSyn-EGFP (2.0 × 1013 GC per ml) for electrophysiology in acute brain slices (Extended Data Fig. 3m–o); AAV2/1-eSyn-NES-jRGECO1a (3.0 × 1013 GC per ml) and AAV2/1-eSyn-RCaMP3 (2.0 × 1013 GC per ml) for one-photon and two-photon calcium imaging in the barrel cortex (Fig. 3d–n); AAV2/1-eSyn-NES-jRGECO1a (1.0 × 1013 GC per ml) and AAV2/1-eSyn-RCaMP3 (1.0 × 1013 GC per ml) for two-photon mesoscale Ca2+ imaging (Fig. 3o,p); AAV2/1-eSyn-NES-jRGECO1a (1.0 × 1013 GC per ml) and AAV2/1-eSyn-RCaMP3 (1.0 × 1013 GC per ml) for inclusion counting (Extended Data Fig. 5a,b); AAV2/1-eSyn-cAMPinG1-ST (1.0 × 1013 GC per ml), AAV2/1-eSyn-cAMPinG1mut-ST (1.0 × 1013 GC per ml) and AAV2/1-eSyn-RCaMP3 (1.0 × 1013 GC per ml) for two-photon imaging in the V1 (Figs. 4 and 5a–g). AAV2/1-eSyn-cAMPinG1-NE (7.0 × 1012 GC per ml) and AAV2/1-eSyn-G-Flamp1 (7.0 × 1012 GC per ml) for confocal imaging in fixed tissues (Extended Data Fig. 5f, g); AAV2/1-hSyn-iCre (2.0 × 1010 GC per ml), AAV2/1-CAG-DIO-cAMPinG1-NE (3.0 × 1013 GC per ml), AAV2/1-CAG-DIO-RCaMP3 (1.0 × 1013 GC per ml, for an infection marker) and AAV2/1-CAG-DIO-ChRmine-mScarlet-Kv2.1 (1.0 × 1012 GC per ml) for cAMP imaging and optogenetic stimulation (Fig. 5h–k); AAV2/1-eSyn-cAMPinG1-NE (7.0 × 1012 GC per ml) and AAV2/1-eSyn-RCaMP3 (1.0 × 1013 GC per ml, for an infection marker) for fiber photometry in the V1 (Extended Data Fig. 6e–g); AAV2/1-gfaABC1D-cAMPinG1-NE (1.0 × 1013 GC per ml) and AAV2/1-gfaABC1D-RCaMP3 (1.0 × 1013 GC per ml) for astrocyte imaging (Extended Data Fig. 6h–j). AAV2/1-eSyn-G-Flamp1 (3.0 ×1013 GC per ml) for two-photon imaging in the V1 (note that the G-Flamp1 fluorescence diminishes when injecting a mixture of G-Flamp1 and RCaMP3 AAVs for coexpression; Extended Data Fig. 7c,d); AAV2/1-eSyn-cAMPinG1-NE (7.0 × 1012 GC per ml), AAV2/1-eSyn-cAMPinG1mut-NE (7.0 × 1012 GC per ml) or AAV2/1-eSyn-G-Flamp1 (7.0 × 1012 GC per ml) and AAV2/1-eSyn-RCaMP3 (1.0 × 1013 GC per ml) for fiber photometry in the dorsal striatum (Extended Data Fig. 8).
Stereotaxic virus injection was performed to C57BL/6N male mice aged 4–6 weeks anesthetized by the anesthetic mixture described above except for two-photon mesoscale imaging. A2A-Cre transgenic mice were used for the slice experiments for local dopamine application (Extended Data Fig. 3e–h). A micropipette was inserted into the right V1 (A/P −3.85 mm, M/L +2.7 mm from bregma, D/V −0.30 mm from the pial surface), the barrel cortex (A/P −1.0 mm, M/L −3.0 mm from bregma, D/V −0.20 mm from the pial surface), the right dorsal striatum (A/P +0.5 mm, M/L +1.8 mm from bregma, D/V −1.5 mm from the pial surface), the nucleus accumbens (A/P +1.3 mm, M/L ±1.25 mm from bregma, D/V −4.5 mm) or the medial prefrontal cortex (A/P +1.8 mm, M/L +0.3 mm from bregma, D/V −2.4 mm). Then, the virus solution (volume: 500−1,000 nl) was injected. Carprofen (5 mg per kg of body weight, Zoetis) was administered intraperitoneally just after the injection experiment. Mice were subjected to imaging after 4–12 weeks of the injection.
cAMP imaging in acute brain slices
For cAMP imaging with forskolin/IBMX bath application (Extended Data Fig. 3a–d), AAV (AAVPHP.eB-CaMKII-Cre and AAV2/1-CAG-DIO-cAMPinG1, or AAV2/1-CAG-DIO-cAMPinG1mut) was injected into the V1 at a total volume of 500 nl (ref. 52). For cAMP imaging with local dopamine application (Extended Data Fig. 3e–h), AAV (AAV2/1-eSyn-cAMPinG1mut-NE, AAV-CaMKII (0.3 kb)-loxFAS-H2B-mCherry-loxFAS) was injected into the nucleus accumbens at a total volume of 1,000 nl. After 2 weeks of expression, mice were killed by rapid decapitation after anesthesia with isoflurane. The brains were immediately extracted and immersed in gassed (95% O2/5% CO2) and ice-cold solution containing: 220 mM sucrose, 3 mM KCl, 8 mM MgCl2, 1.25 mM NaH2PO4, 26 mM NaHCO3 and 25 mM glucose. Acute coronal brain slices (280 μm thick) of the visual cortex were cut in gassed ice-cold solution with a vibratome (VT1200, Leica). Brain slices were then transferred to an incubation chamber containing gassed artificial cerebrospinal fluid (ACSF) containing: 125 mM NaCl, 2.5 mM KCl, 1.25 mM NaH2PO4, 26 mM NaHCO3, 1 mM CaCl2, 2 mM MgCl2 and 20 mM glucose at 34 °C for 30 min and subsequently maintained at room temperature before transferring them to the recording chamber and perfused with the ACSF solution described above, except using 2 mM CaCl2 and 1 mM MgCl2 at 30–32 °C. cAMP imaging was performed with an upright microscope (BX61WI, Olympus) equipped with an FV1000 laser-scanning system (FV1000, Olympus) and a water-immersion ×60 objective (N.A.: 1.0, Olympus), a femtosecond laser (MaiTai, Spectra-Physics) and a GaAsP detector (Hamamatsu Photonics) with a 500–550-nm emission filter (Semrock). The laser was tuned at 940 nm (2 mW at the front aperture of the objective). For cAMP imaging with forskolin/IBMX bath application (Extended Data Fig. 3a–d), images (105.6 × 105.6 µm2, 640 × 640 pixels) were taken every 30 s. During the imaging, forskolin (067-02191, Wako) and IBMX (3758, Tocris) were added to a final concentration of 25 µM and 50 µM, respectively. For cAMP imaging with local dopamine application (Extended Data Fig. 3e–h), images were taken at 2 Hz. During the imaging, 1 µM dopamine was microperfused with a micropipette.
For cAMP imaging with norepinephrine bath application (Extended Data Fig. 3i–l), in utero electroporation was performed as described above. Four weeks after birth, mice were killed by rapid decapitation after anesthesia with the anesthetic mixture described above. The brains were immediately extracted and immersed in gassed (95% O2/5% CO2) and ice-cold solution containing: 222 mM sucrose, 3.6 mM KCl, 2 mM MgCl2, 2 mM CaCl2, 1.5 mM NaH2PO4 and 27 mM NaHCO3. Acute coronal brain slices (300 μm thick) of the visual cortex were cut in gassed ice-cold solution with a vibratome (VT1200, Leica). Brain slices were then transferred to an incubation chamber containing gassed ACSF containing: 126 mM NaCl, 3 mM KCl, 1.1 mM NaH2PO4, 26 mM NaHCO3, 2 mM CaCl2, 2 mM MgCl2 and 10 mM glucose at room temperature for 30 min. The brain slices were transferred to the recording chamber and perfused with the ACSF solution described above. cAMP imaging was performed with FVMPE-RS (Olympus) equipped with a water-immersion ×25 objective lens (N.A.: 1.05, Olympus), a femtosecond laser (Insight DS+, Spectra-Physics) and two GaAsP detectors (Hamamatsu Photonics) with 495–540-nm emission filters (Olympus). The laser was tuned at 940 nm. Images (339 × 339 µm2, 256 × 256 pixels) with 36 optical planes with planes spaced 2 µm apart in depth were collected every 31 s. During the imaging, norepinephrine (A0906, Tokyo Chemical Industry) was added to a final concentration of 0.5 µM.
Electrophysiology for characterization of cAMPinG1
For characterization of cAMPinG1 in acute brain slices (Extended Data Fig. 3m–o), AAV (AAV2/1-eSyn-cAMPinG1-NE, AAVPHP.eB-hSyn-EGFP) was injected into the medial prefrontal cortex of mice aged 8 weeks at a volume of 500 nl. After 2 weeks of expression, mice were decapitated, and acute brain slices were prepared as described above. Slices were perfused with oxygenated ACSF (125 mM NaCl, 2.5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 1.25 mM NaH2PO4, 26 mM NaHCO3, 20 mM glucose and 200 μM Trolox). Whole-cell recordings were performed by 5–6 MΩ glass pipettes. Patch pipettes were filled with an internal solution (120 mM potassium gluconate, 20 mM KCl, 10 mM disodium phosphocreatine, 4 mM ATP (magnesium salt), 0.3 mM GTP (sodium salt), 10 mM HEPES (pH 7.25, 293 mOsm). Electrophysiological data were acquired using a patch-clamp amplifier (MultiClamp 700B, Molecular devices) and stored using a Digidata 1440A converter and pCLAMP software (Molecular Devices). To assess the excitability, the spike number was measured by injecting pulses of increased intensity in steps of 25 pA (from 0 to 250 pA, 500 ms duration). For miniature excitatory postsynaptic current measurement, tetrodotoxin (0.2 μM to a final concentration), APV (50 μM) and picrotoxin (25 μM) were added and membrane potential voltage clamped at −70 mV after correction of liquid-junction potential was used.
Tissue preparation
Tissue blocks were cut into 50-μm-thick slices with a cryostat (CM1950, Leica) and floated in PBS. For nuclear staining, brain slices were incubated with DAPI for 10 min at room temperature before being mounted on slides. Imaging was performed using an LSM 880 confocal microscope with a ×20 objective lens and 405-nm, 488-nm and 561-nm lasers. For inclusion counting of Ca2+ sensors, images (142 × 142 µm2, 512 × 512 pixels) were acquired across multiple optical planes, each spaced 2 µm apart in depth from L2/3 neurons in the V1.
Simultaneous Ca2+ imaging and whole-cell recordings in acute brain slices
AAV (AAV2/1-eSyn-jRGECO1a, AAV2/1-eSyn-RCaMP3) was injected into the barrel cortex (A/P −1.0 mm, M/L −3.0 mm from the bregma, D/V −0.2 mm from the pial surface) at 20 nl min−1 at a volume of 500 nl. After 4 weeks of expression, mice were killed by rapid decapitation after anesthesia with pentobarbital (100 mg per kg body weight). The brains were immediately extracted and immersed in gassed (95% O2/5% CO2) and ice-cold ACSF containing: 124 mM NaCl, 2.5 mM KCl, 1.25 mM NaH2PO4, 26 mM NaHCO3, 2 mM CaCl2, 2 mM MgCl2 and 10.1 mM glucose. Acute coronal brain slices (300 μm thick) of the barrel cortex were cut in gassed, ice-cold ACSF with a vibratome (VT1200S, Leica). Brain slices were then transferred to an incubation chamber containing gassed ACSF at 30 °C for 60 min and subsequently maintained at room temperature before transferring them to the recording chamber at 35 °C.
Whole-cell recordings were performed in the L2/3 pyramidal neurons of the barrel cortex with glass recording electrodes (5–8 MΩ) filled with the intracellular solution containing: 130 mM K-gluconate, 4 mM NaCl, 10 mM HEPES, 4 mM Mg-ATP, 0.3 mM Na-GTP, 7 mM dipotassium-phosphocreatine and pH adjusted to 7.0 with potassium hydroxide (296 mOsm). Electrophysiological data were acquired using a patch-clamp amplifier (MultiClamp 700B, Molecular devices) filtered at 10 kHz and sampled at 20 kHz. Single action potentials were evoked by injecting a series of current pulses (2 ms in duration) through the patch pipette. Each trial was repeated, and the mean value was presented.
Calcium imaging was performed using an upright microscope (BX51WI, Olympus) with a water-immersion ×40 objective lens (N.A.: 0.8, Olympus). To acquire RCaMP3 and jRGECO1a images with LED light (MCWHLP1, Thorlabs), a U-MWIG3 fluorescence mirror unit (Olympus) was used. Fluorescent images were captured by a sCMOS camera (Orca-Flash 4.0 v3, Hamamatsu Photonics) controlled by HC Image software (Hamamatsu Photonics). Images were acquired at 50 Hz with 1 × 1 binning.
Simultaneous calcium imaging and cell-attached recordings in vivo
AAV (AAV2/1-eSyn-jRGECO1a, AAV2/1-eSyn-RCaMP3) was injected into the barrel cortex (A/P −1.0 mm, M/L −3.0 mm from the bregma, D/V −0.2 mm from the pial surface) at 20 nl min−1 at a volume of 500 nl. After 4 weeks of expression, mice were head-fixed and anesthetized with isoflurane (~1.5–2.0%) throughout the experiment, and body temperature was kept at 37 °C with a heating pad. A craniotomy was made in the barrel cortex. The exposed brain was covered with 1.5% agarose in ACSF containing the following: 150 mM NaCl, 2.5 mM KCl, 10 mM HEPES, 2 mM CaCl2 and 1 mM MgCl2, pH 7.3. A glass coverslip was then placed over the agarose to suppress the brain motion artifacts. A glass electrode (5–8 MΩ) was filled with ACSF containing Alexa 488 (200 µM). jRGECO1a or RCaMP3-expressing neurons were targeted using two-photon microscopy (Movable Objective Microscope, Sutter) with a tunable laser (InSight X3, Spectra-Physics) and a water-immersion ×16 objective lens (N.A.: 0.80, Nikon). Fluorescence signals were collected using a GaAsP photomultiplier tube (Hamamatsu Photonics) with a 590–660-nm emission filter. After establishing the cell-attached configuration (20–100 MΩ seal), simultaneous spike recording and calcium imaging were performed at the soma (sampling rate = 30 Hz, 512 × 512 pixels). Electrophysiological data were acquired using a patch-clamp amplifier (MultiClamp 700B; Molecular devices) in current-clamp mode, filtered at 10 kHz, and sampled at 20 kHz. The laser was tuned to 1,040 nm (40 mW at the front aperture of the objective).
Fiber photometry
A 400-µm-diameter mono fiber-optic cannula (Kyocera) was implanted. A custom-made metal head plate was attached to the skull with dental cement. Mice were subjected to imaging after more than 2 days of the surgery.
Dual-color fiber photometry for green cAMP sensors and RCaMP3 was performed using the GCaMP and Red Fluorophore Fiber Photometry System (Doric) with 405-nm, 470-nm and 560-nm LED, 500–540-nm and 580–680-nm emission filter, and 400-µm-diameter 0.57-N.A. Mono Fiber-optic Patch Cords (Doric). Photometry data were recorded at a sampling rate of 30 Hz by lock-in amplifier detection. Mice were head-fixed during the recordings.
For cAMP imaging in the V1, on the first day, just after the first recording for 30-s forced running, 25 mg per kg body weight propranolol (168-28071, Wako) or mock solution was intraperitoneally administered. Thirty minutes later, the second recording was performed. On the subsequent day, the same two recordings were performed for the same mice, but with the reversed treatments. Half of the mice received propranolol on the first day and the other half received mock solution on the first day.
Cranial window implantation
Mice were anesthetized by the anesthetic mixture described above. Before surgery, dexamethasone sodium phosphate (2 mg per kg body weight, Wako) and carprofen (5 mg per kg of body weight) were administered to prevent inflammation and pain. During surgery, mice were put on a heating pad, and body temperature was kept at 37 °C. A custom-made stainless-steel head plate was fixed to the skull using cyanoacrylate adhesive and dental cement (Sun Medical) above the right visual cortex. A craniotomy was drilled with a 2.5-mm diameter, and the brain was kept moist with saline. A cover glass (3-mm diameter, no. 0 thickness, Warner Instruments) was placed over the craniotomy site with surgical adhesive glue (Aron Alpha A, Sankyo)49,50. The mice were subjected to imaging more than 18 h after the surgery.
In vivo two-photon imaging
In vivo two-photon imaging was performed with an FVMPE-RS (Olympus) equipped with a water-immersion ×25 objective (N.A.: 1.05, Olympus), a femtosecond laser (Insight DS+, Spectra-Physics) and two GaAsP detectors (Hamamatsu Photonics) with 495–540-nm and 575–645-nm emission filters (Olympus). For somatic cAMP imaging of cAMPinG1, cAMPinG1mut and G-Flamp1 expressed by in utero electroporation, images (339 × 339 µm2, 512 × 512 pixels, single optical section) were collected at 15 Hz in the awake condition. The laser was tuned to 940 nm (48.6 mW at the front aperture of the objective). For cAMPinG1-NE spine imaging, images (28.8 × 38.4 µm2, 96 × 128 pixels, single optical section) were collected at 7.5 Hz in the condition anesthetized lightly by isoflurane (0.5% vol/vol). The laser power was 23.5 mW at the front aperture of the objective. For RCaMP3 and cAMPinG1-ST imaging, sequential excitation at 940 nm and 1,040 nm was used for dual-color imaging. Images (339 × 339 µm2, 512 × 512 pixels) with three optical planes with plane spaced 30 µm apart in depth were collected at 3.4 Hz per plane using a piezo objective scanner (Olympus). The laser power was set to 47.6 mW for 940 nm excitation, and to 113.7 mW for 1,040 nm excitation. The imaging with visual stimuli (Fig. 5a–g) was followed by the imaging during forced running (Fig. 4) on the same cell population. For cAMP and Ca2+ imaging in astrocytes, images (288 × 384 µm2, 192 × 256 pixels) were collected at 1.6 Hz in the awake condition. The laser power for 940 nm of excitation was set to 26.5 mW, and for 1,040 nm of excitation, it was set to 52.2 mW. For single-cell cAMP imaging with optical stimulation using soma-targeted ChRmine, images (86.4 × 115.2 µm2, 192 × 256 pixels) were collected at 3.2 Hz in the awake condition with 940 nm of excitation. Next, 1,040-nm two-photon excitation was used for 4-s, 16-Hz spiral scanning with 11-µm diameter. The imaging with a 940-nm laser was temporally stopped during the optical excitation. The laser power for 940-nm excitation was set to 4.1 mW, and for 1,040 nm of photostimulation, it was set to 39.8 mW.
Two-photon mesoscale Ca2+ imaging with FASHIO-2PM
AAV (AAV2/1-eSyn-RCaMP3) was injected into the neonatal somatosensory cortex53. After 8 weeks of AAV injection, a 4.5-mm-diameter craniotomy was performed over an area including the primary somatosensory area of the right hemisphere. A head plate was also fixed to the skull above the cerebellum.
Two-photon imaging was performed with FASHIO-2PM29 equipped with a femtosecond laser (Chameleon Discovery, Coherent). The laser was tuned to 1,040 nm. The field of view was 3.0 × 3.0 mm2 (2,048 × 2,048 pixels). The sampling rate was 7.5 Hz. Laser power of 270 mW and 360 mW at the front of the objective lens was used to observe L2/3 and L5 neurons of awake mice, respectively.
Physical stimulation
Airpuff stimuli (2 Hz, 0.1 s duration, 40 times) were generated using a microinjector (BEX). For the forced running task, mice were head-fixed, and a custom-made treadmill was turned on during recordings. Moving grating stimuli were generated using the PsychoPy function in Python34. The gratings were presented with an LCD monitor (19.5 inches, 1,600 × 900 pixels, Dell), placed 25 cm in front of the center of the left eye of the mouse. Each stimulus trial consisted of a 4-s blank period (uniform gray at mean luminance) followed by a 4-s drifting sinusoidal grating (0.04 cycles per degree, 2 Hz temporal frequency). Eight drifting directions (separated by 45°, in order from 0° to 315°) were used. The timing of each moving grating stimulus and the initiation of imaging were monitored with a data acquisition module (USB-6343, National Instruments) controlled by LabVIEW (2021).
Analysis of in vitro experiments
In vitro fluorometry analysis was performed using Python (https://www.python.org/) and Excel (Microsoft). For both Ca2+ and cAMP sensors, the Kd value and Hill coefficient were calculated by fitting according to the Hill equation. For both Ca2+ and cAMP sensors, the pKa values were calculated by the inflection point of a sigmoid fit to fluorescence versus pH. For in vitro kinetics analysis (Extended Data Fig. 2g,h), half-rise time and half-decay time were calculated by single exponential fitting.
Image analysis
Image analyses were performed with ImageJ (National Institutes of Health) and Python. For somatic cAMP imaging of cAMPinG1, cAMPinG1mut and G-Flamp1 expressed by in utero electroporation (Fig. 2a–f), somatic cAMP imaging expressed by AAV (Extended Data Fig. 6a–d and Extended Data Fig. 7) and simultaneous RCaMP3 and cAMPinG1-ST imaging (Figs. 4 and 5a–g and Extended Data Fig. 7e), motion correction was performed with Suite2p toolbox (https://github.com/MouseLand/suite2p/)54. For cAMPinG1-NE spine imaging (Fig. 2g–i), mesoscale Ca2+ imaging (Fig. 3o,p and Extended Data Fig. 5c,d), Ca2+ and cAMP imaging in astrocytes (Extended Data Fig. 6h–j) and cAMP imaging with optical stimulation using soma-targeted ChRmine (Fig. 5h–k), motion correction was performed with TurboReg55.
Region of interest (ROI) detection for in vivo Ca2+ imaging was performed with Suite2p. ROI detection for HEK cell live imaging and in vivo cAMP imaging were performed with ImageJ and Cellpose56. ROIs for cAMPinG1-NE spine imaging (Fig. 2g–i), Ca2+ and cAMP imaging in astrocytes (Extended Data Fig. 6h–j) and cAMPinG1-NE imaging with soma-targeted ChRmine (Fig. 5h–k) were drawn manually. The dynamic range was calculated as ΔF/F = (F – F0)/F0, where F0 was the average fluorescence intensity before stimulations after the subtraction of background fluorescence. No bleaching correction was performed in any analyses except Ca2+ imaging in acute brain slices (Fig. 3d–h). No fluorescence cross-talk correction was performed.
For cAMP imaging using acute brain slices (Extended Data Fig. 3), background subtraction was performed before calculating ΔF/F. ROIs were drawn manually around somata (Extended Data Fig. 3a–h) or with Cellpose (Extended Data Fig. 3i–l) in the time-series-averaged image. ΔF/F was calculated as (F − F0)/F0, where F is the fluorescence intensity at any timepoint and F0 is the average fluorescence before the drug application.
For in vivo cAMPinG1-NE spine imaging (Fig. 2g–i), the period after stimulation was defined as a 15-s period starting 10 s after the end of airpuff stimulation.
For two-photon Ca2+ imaging in HEK293T cells (Fig. 3c), ROIs drawn based on mCerulean images with Cellpose were used for both Ca2+ sensor and mCerulean images. The red fluorescence intensity was divided by mCerulean fluorescence intensity in each cell for normalization.
For Ca2+ imaging using acute brain slices (Fig. 3d–h), background subtraction and bleach correction were performed before calculating ΔF/F. ROIs were manually selected around somata in the time-series-averaged image. ΔF/F was calculated as (F − F0)/F0, where F is the fluorescence intensity at any timepoint and F0 is the resting baseline fluorescence measured 200 ms before stimulation. The peak amplitude was defined as the maximum value of ΔF/F after the stimuli. The rise and decay curves were fit to a single exponential. The rise time was defined as the time from the beginning of the stimulus to the timepoint of the peak fluorescence amplitude. The half-decay time was defined as the time from the maximum value of ΔF/F to half of that value.
For simultaneous Ca2+ imaging and cell-attached recordings in vivo (Fig. 3i–n), ROIs were manually selected around somata in the time-series-averaged image. ΔF/F was calculated as (F−F0)/F0, where F is the fluorescence intensity at any timepoint and F0 is resting baseline fluorescence measured 200 ms before the action potentials. Action potentials were detected by cell-attached recording of the signal. Spike events (1–4 action potentials) were identified, ensuring that no other action potentials occurred in the 1-s period before and after the first action potential. The rise and decay curves were fit to a single exponential. The half-rise time was defined as the time from the beginning of the stimulus to the timepoint of the half of the peak fluorescence amplitude. The half-decay time was defined as the time from the maximum value of ΔF/F to half of that value.
For in vivo Ca2+ and cAMP imaging (Figs. 4 and 5a–g and Extended Data Figs. 6a–d and 7), ROIs for RCaMP3 and cAMP indicators were drawn independently using Suite2p and Cellpose, respectively. For simultaneous RCaMP3 and cAMPinG1-ST imaging (Figs. 4 and 5a–g), the cells that had ROIs for both RCaMP3 and cAMPinG1-ST were selected for further analysis. Because the imaging using visual stimulus (Fig. 5a–g) was followed by the imaging using forced running (Fig. 4) on the same cell population, the same ROIs were used for both analyses. Motion-related neurons were defined as neurons that showed Ca2+ ΔF/F values of more than 0.3 during the running period (Fig. 4). Ca2+ responses to 4 s of visual stimulation were defined as averaged ΔF/F during the 4-s stimulation. cAMP responses to 4 s of visual stimulation were defined as averaged ΔF/F during 2-s periods that started 2 s after the end of the visual stimulus (Fig. 5a–d). Neurons that responded to repetitive 8-s visual stimuli were defined as neurons that showed Ca2+ ΔF/F values of more than 0.3 during the stimulation period (Fig. 5e–g). The period after repetitive visual stimuli was defined as a 40-s period starting after the end of the stimuli. The DSI was calculated for cells showing Ca2+ responses. The preferred direction (θpref) of each cell was defined as the stimulus that induced the largest Ca2+ ΔF/F. The DSI was defined as DSI = (Rpref – Rpref+π)/(Rpref + Rpref+π), where Rpref and Rpref+π are ΔF/F values at the preferred (θpref) and the opposite (θpref + π) directions, respectively. Imaging frames with notable motion artifacts were removed and supplied with the preceding frames.
For cAMPinG1-NE imaging with soma-targeted ChRmine (Fig. 5k), cAMP ΔF/F was defined as averaged ΔF/F during a 10-s period starting 20 s after the end of optical stimulation.
Analysis of fiber photometry
Fiber photometry analysis was performed using Python. The cAMPinG1 signal was calculated as follows: (470-nm signal)/(405-nm signal). The 560-nm signal was recognized as the RCaMP3 signal.
For imaging in the V1 (Extended Data Fig. 6e–g), ΔR/R was calculated as (R − R0)/R0, where F is mean fluorescence intensity in the last 10 s of the running period and F0 is the mean fluorescence intensity in 10 s before the start of running. ΔR/R (pre) and ΔR/R (post) were defined as ΔR/R in response to running before and after the intraperitoneal administration, respectively.
For imaging in the dorsal striatum (Extended Data Fig. 8), the period during stimulation was defined as a 10-s running period, and the period after stimulation was defined as 10 s after the end of the running period.
Statics and reproducibility
All experiments were conducted with at least two biological replicates, often more, involving independent transfection and mice. All statistical analyses of the acquired data were performed with Python. For each figure, a statistical test matching the structure of the experiment and the structure of the data was employed. All tests were two tailed. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant (P > 0.05) for all statistical analyses presented in figures. No statistical tests were done to predetermine the sample size. Data acquirement and analysis were not performed blind to the conditions of the experiments. Experimental sample sizes are mentioned in the figure panel and legends.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
The cAMPinG1 and RCaMP3 sequences are available from GenBank (accession number: LC728270 (cAMPinG1) and LC728271 (RCaMP3)). The plasmids generated in this study are available from RIKEN Bio-Resource Center (https://dna.brc.riken.jp/en/) with the following catalog numbers: pN1-cAMPinG1 (RDB19691), pAAV-eSyn-cAMPinG1-NE-WPRE (RDB19692), pAAV-eSyn-cAMPinG1-ST-WPRE (RDB19693) and pAAV-eSyn-RCaMP3-WPRE (RDB19694). Source data are provided with this paper.
References
Kandel, E. R., Dudai, Y. & Mayford, M. R. The molecular and systems biology of memory. Cell 157, 163–186 (2014).
Adams, S. R., Harootunian, A. T., Buechler, Y. J., Taylor, S. S. & Tsien, R. Y. Fluorescence ratio imaging of cyclic AMP in single cells. Nature 349, 694–697 (1991).
Massengill, C. I., Day-Cooney, J., Mao, T. & Zhong, H. Genetically encoded sensors towards imaging cAMP and PKA activity in vivo. J. Neurosci. Methods 362, 109298 (2021).
Harada, K. et al. Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Sci. Rep. 7, 1–9 (2017).
Oe, Y. et al. Distinct temporal integration of noradrenaline signaling by astrocytic second messengers during vigilance. Nat. Commun. 11, 471 (2020).
Zhang, S. X. et al. Hypothalamic dopamine neurons motivate mating through persistent cAMP signalling. Nature 597, 245–249 (2021).
Wang, L. et al. A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging. Nat. Commun. 13, 5363 (2022).
Massengill, C. I. et al. Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat. Methods 19, 1461–1471 (2022).
Kawata, S., Mukai, Y., Nishimura, Y., Takahashi, T. & Saitoh, N. Green fluorescent cAMP indicator of high speed and specificity suitable for neuronal live-cell imaging. Proc. Natl Acad. Sci. USA 119, e2122618119 (2022).
Liu, W., Liu, C., Ren, P.-G., Chu, J. & Wang, L. An improved genetically encoded fluorescent camp indicator for sensitive cAMP imaging and fast DRUG SCreening. Front. Pharmacol. 13, 902290 (2022).
Tewson, P. H., Martinka, S., Shaner, N. C., Hughes, T. E. & Quinn, A. M. New DAG and cAMP sensors optimized for live-cell assays in automated laboratories. J. Biomol. Screen. 21, 298–305 (2016).
Ludwig, A., Zong, X., Jeglitsch, M., Hofmann, F. & Biel, M. A family of hyperpolarization-activated mammalian cation channels. Nature 393, 587–591 (1998).
Zhang, P. et al. Structure and allostery of the PKA RII tetrameric holoenzyme. Science 335, 712–716 (2012).
Zhong, H. et al. Subcellular dynamics of Type II PKA in neurons. Neuron 62, 363–374 (2009).
Canaves, J. M. & Taylor, S. S. Classification and phylogenetic analysis of the cAMP-dependent protein kinase regulatory subunit family. J. Mol. Evol. 54, 17–29 (2002).
Kim, C., Cheng, C. Y., Saldanha, S. A. & Taylor, S. S. PKA-I holoenzyme structure reveals a mechanism for cAMP-dependent activation. Cell 130, 1032–1043 (2007).
Su, Y. et al. Regulatory subunit of protein kinase A: structure of deletion mutant with cAMP binding domains. Science 269, 807–813 (1995).
Lorenz, R. et al. Mutations of PKA cyclic nucleotide-binding domains reveal novel aspects of cyclic nucleotide selectivity. Biochem. J. 474, 2389–2403 (2017).
Wu, J., Brown, S., Xuong, N.-H. & Taylor, S. S. RIalpha subunit of PKA: a cAMP-free structure reveals a hydrophobic capping mechanism for docking cAMP into site B. Structure 12, 1057–1065 (2004).
Dagliyan, O. et al. Engineering extrinsic disorder to control protein activity in living cells. Science 354, 1441–1444 (2016).
Ohkura, M., Sasaki, T., Kobayashi, C., Ikegaya, Y. & Nakai, J. An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS ONE 7, e39933 (2012).
Chen, Y. et al. Soma-targeted imaging of neural circuits by ribosome tethering. Neuron 107, 454–469 (2020).
Odaka, H., Arai, S., Inoue, T. & Kitaguchi, T. Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One 9, e100252 (2014).
Inoue, M. et al. Rational engineering of XCaMPs, a multicolor GECI suite for in vivo imaging of complex brain circuit dynamics. Cell 177, 1346–1360 (2019).
Inoue, M. et al. Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat. Methods 12, 64–70 (2015).
Dana, H. et al. Sensitive red protein calcium indicators for imaging neural activity. Elife 5, e12727 (2016).
Zhao, Y. et al. An expanded palette of genetically encoded Ca2+ indicators. Science 333, 1888–1891 (2011).
Wu, J. et al. Improved orange and red Ca2+ indicators and photophysical considerations for optogenetic applications. ACS Chem. Neurosci. 4, 963–972 (2013).
Ota, K. et al. Fast, cell-resolution, contiguous-wide two-photon imaging to reveal functional network architectures across multi-modal cortical areas. Neuron 109, 1810–1824 (2021).
Ma, L. et al. A highly sensitive a-kinase activity reporter for imaging neuromodulatory events in awake mice. Neuron 99, 665–679 (2018).
Reimer, J. et al. Pupil fluctuations track rapid changes in adrenergic and cholinergic activity in cortex. Nat. Commun. 7, 13289 (2016).
Stringer, C. et al. Spontaneous behaviors drive multidimensional, brainwide activity. Science 364, 255 (2019).
Lee, Y., Su, M., Messing, A. & Brenner, M. Astrocyte heterogeneity revealed by expression of a GFAP-LacZ transgene. Glia 53, 677–687 (2006).
Chen, T.-W. et al. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499, 295–300 (2013).
Marshel, J. H. et al. Cortical layer-specific critical dynamics triggering perception. Science 365, eaaw5202 (2019).
Parker, J. G. et al. Diametric neural ensemble dynamics in parkinsonian and dyskinetic states. Nature 557, 177–182 (2018).
Wang, Q., Dong, X., Lu, J., Hu, T. & Pei, G. Constitutive activity of a G protein-coupled receptor, DRD1, contributes to human cerebral organoid formation. Stem Cells 38, 653–665 (2020).
Lin, X. et al. Structural basis of ligand recognition and self-activation of orphan GPR52. Nature 579, 152–157 (2020).
Mironov, S. et al. Imaging cytoplasmic cAMP in mouse brainstem neurons. BMC Neurosci. 10, 29 (2009).
Zhang, J. Z. et al. Phase separation of a PKA regulatory subunit controls cAMP compartmentation and oncogenic signaling. Cell 182, 1531–1544 (2020).
Anton, S. E. et al. Receptor-associated independent cAMP nanodomains mediate spatiotemporal specificity of GPCR signaling. Cell 185, 1130–1142 (2022).
Omori, K. & Kotera, J. Overview of PDEs and their regulation. Circ. Res. 100, 309–327 (2007).
Yagishita, S. et al. A critical time window for dopamine actions on the structural plasticity of dendritic spines. Science 345, 1616–1620 (2014).
Hauser, A. S. et al. Common coupling map advances GPCR-G protein selectivity. Elife 11, e74107 (2022).
Shen, Y. et al. A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578. BMC Biol. 16, 9 (2018).
Kroeze, W. K. et al. PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. Nat. Struct. Mol. Biol. 22, 362–369 (2015).
Imayoshi, I. et al. Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. Science 342, 1203–1208 (2013).
Shcherbakova, D. M. & Verkhusha, V. V. Near-infrared fluorescent proteins for multicolor in vivo imaging. Nat. Methods 10, 751–754 (2013).
Sakamoto, M. et al. In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protoc. 3, 101421 (2022).
Sakamoto, M. et al. A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Rep. Methods 2, 100168 (2022).
Kawashima, T. et al. Functional labeling of neurons and their projections using the synthetic activity-dependent promoter E-SARE. Nat. Methods 10, 889–895 (2013).
Chan, K. Y. et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems. Nat. Neurosci. 20, 1172–1179 (2017).
Oomoto, I. et al. Protocol for cortical-wide field-of-view two-photon imaging with quick neonatal adeno-associated virus injection. STAR Protoc. 2, 101007 (2021).
Pachitariu, M. et al. Suite2p: beyond 10,000 neurons with standard two-photon microscopy. Preprint at bioRxiv https://doi.org/10.1101/061507 (2016).
Thevenaz, P., Ruttimann, U. E. & Unser, M. A pyramid approach to subpixel registration based on intensity. IEEE Trans. Image Process. 7, 27–41 (1998).
Stringer, C., Wang, T., Michaelos, M. & Pachitariu, M. Cellpose: a generalist algorithm for cellular segmentation. Nat. Methods 18, 100–106 (2021).
Acknowledgements
We thank the following researchers for kindly sharing their reagents: H. Bito, The University of Tokyo (eSyn promoter, cHS4, XCaMP-R and pCAG-Flpo plasmids); N. Saito, Doshisha University (gCarvi plasmid, and a protocol about kinetics analysis); T. Jacks, Massachusetts Institute of Technology (Addgene plasmid, 12093); V. Verkhusha, Albert Einstein College of Medicine (Addgene plasmid, 45457); J. Kim, Korea Institute of Science and Technology (Addgene plasmid, 51904); B. Khakh, the University of California, Los Angeles (Addgene plasmid, 52924); T. Kitaguchi, Tokyo Institute of Technology (Addgene plasmid, 73938); D. Kim, Janelia Research Campus (Addgene plasmid, 100854); V. Gradinaru, California Institute of Technology (Addgene plasmid, 103005); R. Campbell, The University of Tokyo (Addgene plasmid, 105864); J. Garrison, Buck Institute (Addgene plasmid, 158777); H. Zhong, Vollum Institute (Addgene plasmid, 182283); and B. Roth, University of North Carolina at Chapel Hill (Addgene kit, 1000000068). We also thank Y. Kato for technical assistance and members of the laboratories of M.S. and I.I. for their support and comments.
This work was supported in part by grants from Precursory Research for Embryonic Science and Technology (PRESTO)-JST (JPMJPR1906 to M.S.), ACT-X-JST (JPMJAX211K to T.Y.), CREST-JST (JPMJCR1921 to I.I.), Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) (JP19dm0207079 to S.M., JP19dm0207090 to I.I., JP19dm0207069 to S.Y., JP15dm0207001 to M.M., JP19dm0207080 to K.K. and JP17dm0207059 to M.S.), Interdisciplinary and Emerging Brain Research Program (iBrain/MINDS) (JP21wm0525018 to S.Y. and JP21wm0525004 to M.S.), Program for Technological Innovation of Regenerative Medicine (JP21bm0704060 to I.I.), AMED-PRIME (JP 23gm6510022 to M.S.), Interstellar Initiative Beyond (JP22jm0610068 to M.S.), JSPS KAKENHI (JP21K15207 to T.Y., JP22H02718 to S.M., JP21H02485 to I.I., JP20H05775 to M.M., JP22H05161 and JP22H00460 to K.K. and JP23H02782, JP22H04922, JP21K19429 and JP20H04122 to M.S.), RIKEN Special Postdoctoral Researchers Program (to H.U.), Inamori Foundation (to M.S.), Takeda Science Foundation (to M.S.), Lotte Foundation (to M.S.), The Konica Minolta Science and Technology Foundation (to M.S.), Tokyo Biochemical Research Foundation (to M.S.), Brain Science Foundation (to M.S.), Mochida Memorial Foundation (to M.S.) and KOSÉ Cosmetology Research Foundation (to M.S.). This work was also supported by Kyoto University Live Imaging Center.
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Authors and Affiliations
Contributions
T.Y. designed and developed cAMP sensors. T.Y. and M.S. designed and developed the RCaMP3. T.Y. performed most of the experiments and analyzed data. S.M. and K.K. performed electrophysiological recordings for RCaMP3 characterization. U.H. and M.M. performed Ca2+ imaging with the FASHIO-2PM. M.T. and S.Y. performed electrophysiological and pharmacological experiments for cAMPinG1 in acute brain slices. T.Y. and M.S. designed the study and wrote the paper with input from all authors. I.I. and M.S. supervised the entire project.
Corresponding authors
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Competing interests
T.Y. and M.S. have filed a patent related to cAMPinG1. The remaining authors declare no competing interests.
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Nature Methods thanks Xiaoke Bi and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Primary Handling Editor: Nina Vogt, in collaboration with the Nature Methods team.
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Extended data
Extended Data Fig. 1 Design of cAMPinG1.
a, Primary structures of mouse PKA regulatory-subunit 1α (PKA-R1α) (top) and cAMPinG1 (bottom). We removed the N-terminal PKA-R1α region, which includes a dimerization/docking domain interacting with scaffold proteins and an inhibitory domain interacting with PKA catalytic subunits (PKA-C), to avoid interaction with these endogenous proteins. Instead, we fused the RSET sequence to the N-terminus of the sensor to promote stable expression. b, Tertiary structures of cAMP-free (left, PDB: 2QCS, catalytic-subunit is hidden) and cAMP-binding (right, PDB: 1RGS) PKA R1α. Note the cpGFP insertion site is close to cAMP in the cAMP-bound three-dimensional structure and exposed on the surface. c, The amino acid sequence of cAMP-binding domains of PKA regulatory-subunit. mR1a, Mus muslucus PKA-RIa; mR1b, Mus musculus PKA-R1b; mR2a, Mus musculus PKA-R2a; mR2b, Mus musculus PKA-R2b; DrosR, Drosophila melanogaster PKA-R; DictR, Dictyostelium discoideum PKA-R; UstiR, Ustilago maydis PKA-R; Sch1R, Schizosaccharomyces pombe PKA-R; ParaR, Paramecium tetraurelia PKA-R.
Extended Data Fig. 2 Characterization of cAMP sensors in vitro.
a, Excitation and emission spectra of cAMP-free and cAMP-saturated G-Flamp1. b, Fluorescence intensities of cAMP sensors in cAMP-free and cAMP-saturated states in HEK293T cell lysate. n = 4 wells in each sensor. Tukey’s post hoc test following one-way ANOVA. gCarvi vs cAMPinG1: P = 4.2 × 10−13; G-Flamp1 vs cAMPinG1: P = 2.4 × 10−2. c, Hill coefficients of cAMP sensors. n = 4 wells in each sensor. Tukey’s post hoc test following one-way ANOVA. Flamindo2 vs gCarvi: P = 6.3 × 10−4; gCarvi vs G-Flamp1: P = 1.3 × 10−3; gCarvi vs cAMPinG1: P = 2.1 × 10−3. d, cGMP and cAMP titration curves of Flamindo2 and cAMPinG1. n = 4 wells in each condition. Flamindo2: cGMP Kd = 21.0 μM, cAMP Kd = 2.5 μM, cAMPinG1: cGMP Kd = 12.2 μM, cAMP Kd = 0.18 μM. e, ΔF/F, cAMP Kd value, and Hill coefficient of cAMPinG1-NE. n = 4 wells in each condition. f, pH titration of cAMP-free and cAMP-saturated cAMP sensors. n = 4 wells in each condition. cAMP-saturated Flamindo2: pKa = 8.2, cAMP-free Flamindo2: pKa = 7.6, cAMP-saturated gCarvi: pKa = 8.2, cAMP-free gCarvi: pKa = 8.5, cAMP-saturated G-flamp1: pKa = 6.9, cAMP-free G-flamp1: pKa = 7.9, cAMP-saturated cAMPinG1: pKa = 7.2, cAMP-free cAMPinG1: pKa = 6.5, cAMPinG1mut: pKa = 7.0. g, In vitro binding kinetics of cAMPinG1 in response to cAMP microperfusion from 0 μM to 2 μM. n = 4 trials. Half rise time = 0.04 s, kon = 8.0 × 106 (M−1s−1). h, In vitro dissociation kinetics of cAMPinG1 in response to cAMP microperfusion from 0.5 μM to 0 μM. n = 4 trials. Half decay time = 0.44 s, koff = 1.6 (s−1). i, Representative images of binding assay of PKA-R1α-mEGFP and cAMPinG1 with PKA-catalytic subunit (Cat)-RFP-CAAX in HEK293T cells. Lines are for quantification of fluorescence distribution for (j). Scale bar, 10 μm. j, Quantification of line fluorescence distribution. The membrane location, as detected by the red fluorescence of PKA-Cat-RFP-CAAX, is set at 0 and 1 on the x-axis. n = 5 cells (PKA-R1α-mEGFP), 5 cells (cAMPinG1). Scale bar, 10 μm. k, Fluorescence lifetime of cAMP sensors and fluorescence proteins expressed in HEK293T cells. n = 8 FOVs in each condition. Unpaired two-tailed t-test. cAMPinG1 in the absence vs presence of 10 μM forskolin: P = 1.8 × 10−5; G-Flamp1 in the absence vs presence of 10 μM forskolin: P = 8.5 × 10−2. All shaded areas and error bars denote SEM.
Extended Data Fig. 3 Characterization of cAMP sensors in acute brain slices.
a, Schematic of AAVs for sparse expression. b, Schematic of imaging settings (left). A representative image (right). Scale bar, 20 μm. c, Traces of cAMPinG1-NE (left) and cAMPinG1mut-NE (right) in response to 25 µM forskolin and 50 µM IBMX. Thin lines denote individual trace, and colored thick lines denote average response. n = 16 neurons in 5 slices (cAMPinG1-NE), 12 neurons in 2 slices (cAMPinG1mut-NE). d, ΔF/F for the forskolin (FSK) and IBMX application. n = 16 neurons in 5 slices (cAMPinG1-NE), n = 12 neurons in 2 slices (cAMPinG1mut-NE). Unpaired two-tailed t-test. P = 1.2 × 10−2. e, Schematic of “Cre-Off” system for expressing cAMP sensors in all neurons and mCherry in dopamine receptor D1-stratal projection neurons (D1R-SPNs) in the striatum. f, Schematic of imaging settings (left) and representative images of cAMPinG1-NE in an acute brain slice (right). Fluorescence in response to 10 s local puff of 1 µM dopamine was recorded in mCherry-positive D1R-SPNs (arrow). Dopamine receptor D2 (D2R)-positive neurons were not labeled with mCherry (arrowhead) due to the expression of Cre recombinase. Scale bar, 10 μm. g, Traces of cAMPinG1-NE to dopamine (left), cAMPinG1-NE to ACSF (left), and G-Flamp1 to dopamine (right). The responses to dopamine were aligned with a single-exponential fitting. Half rise time = 1.4 s (cAMPinG1-NE), 1.4 s (G-Flamp1). n = 7 neurons in 3 slices (cAMPinG1-NE to dopamine), n = 11 neurons in 4 slices (cAMPinG1-NE to ACSF), n = 9 neurons in 4 slices (G-Flamp1 to dopamine). h, ΔF/F in response to dopamine. n = 7 neurons in 3 slices (cAMPinG1-NE), n = 9 neurons in 4 slices (G-Flamp1). Unpaired two-tailed t-test. P = 1.2 × 10−3. i, Schematic of the experimental procedure. j, Schematic of imaging settings (left) and representative images (right). Scale bar, 20 μm. k, Traces of cAMPinG1-NE and G-Flamp1 in response to 0.5 µM noradrenaline. Thin lines denote individual trace, and thick lines denote average response. n = 84 neurons in 4 slices (cAMPinG1-NE), 26 neurons in 4 slices (G-Flamp1). l, ΔF/F in response to noradrenaline. n = 84 neurons in 4 slices (cAMPinG1-NE), 26 neurons in 4 slices from (G-Flamp1). Unpaired two-tailed t-test. P = 4.6 × 10−6. m, Schematic of the experimental procedure. n, Number of spikes evoked by current injection in expressing cAMPinG1-NE and EGFP neurons. n = 8 neurons in each condition. o, mEPSC (miniature excitatory postsynaptic current) frequency (left) and amplitude (right) in cAMPinG1-NE and EGFP expressing neurons. n = 5 neurons in each condition. Unpaired two-tailed t-test. Frequency: P = 2.5 × 10−1; Amplitude: P = 4.9 × 10−1. All shaded areas and error bars denote SEM.
Extended Data Fig. 4 Characterization of red Ca2+ sensors.
a, Primary structures of red Ca2+ sensors. Mutations are indicated in R-GECO1 numbering. b, Fluorescence intensities of red Ca2+ sensors in Ca2+-free and Ca2+-saturated conditions in HEK293T cell lysate. n = 4 wells in each sensor. Tukey’s post hoc test following one-way ANOVA. Ca2+-free jRGECO1a vs Ca2+-free RCaMP3: P = 2.6 × 10−5; Ca2+-free XCaMP-R vs Ca2+-free RCaMP3: P = 1.9 × 10−7. Ca2+-saturated jRGECO1a vs Ca2+-saturated RCaMP3: P = 1.3 × 10−9; Ca2+-saturated XCaMP-R vs Ca2+-saturated RCaMP3: P = 1.4 × 10−12. c-e, Excitation and emission spectra of jRGECO1a, XCaMP-R, and RCaMP3 in Ca2+-free and Ca2+-saturated states. n = 4 wells in each condition. f, Comparison of excitation spectra of red Ca2+ indicators. g-i, Ca2+ titration curves (g), Kd values (h), and Hill coefficients (i) of red Ca2+ sensors. n = 4 wells in each sensor. Tukey’s post hoc test following one-way ANOVA. Kd values (h) of jRGECO1a vs RCaMP3: P = 9.0 × 10−1; XCaMP-R vs RCaMP3: P = 8.0 × 10−6. Hill coefficients (i) of jRGECO1a vs RCaMP3: P = 3.8 × 10−2; XCaMP-R vs RCaMP3: P = 6.1 × 10−8. j, Normalized fluorescence of red Ca2+ sensors in Ca2+-free and Ca2+-saturated states as a function of pH. Ca2+-saturated jRGECO1a: pKa = 6.3, Ca2+-free jRGECO1a: pKa = 9.0, Ca2+-saturated XCaMP-R: pKa = 5.3, Ca2+-free XCaMP-R: pKa = 8.6, Ca2+-saturated RCaMP3: pKa = 6.1, Ca2+-free RCaMP3: pKa = 9.4. n = 4 wells in each condition. k,l, One-photon (k) and two-photon (l) bleaching curves of jRGECO1a, XCaMP-R and RCaMP3 expressed in HEK293T cells. n = 63 neurons (jRGECO1a, one-photon), n = 91 neurons (XCaMP-R, one-photon), n = 76 neurons (RCaMP3, one-photon), n = 94 neurons (jRGECO1a, two-photon), n = 98 neurons (XCaMP-R, two-photon), n = 93 neurons (RCaMP3, two-photon). All shaded areas and error bars denote SEM.
Extended Data Fig. 5 Two-photon mesoscale RCaMP3 imaging in layer 2/3.
a, Schematic of the experimental procedure of two-photon mesoscale Ca2+ imaging using fast-scanning high optical invariant two-photon microscopy (FASHIO-2PM). b, Left: A representative full FOV of FASHIO-2PM. Scale bar, 500 μm. Right: Magnified images and Ca2+ traces of representative 6 somata and 6 dendrites. Scale bar, 10 μm.
Extended Data Fig. 6 In vivo imaging during forced running.
a-c, Representative two-photon images and averaged traces of cAMPinG1-ST (a), cAMPinG1mut-ST (b), and G-Flamp1 (c) in response to forced running. n = 1156 cells in 3 mice (cAMPinG1-ST), n = 905 cells in 4 mice (cAMPinG1mut-ST), n = 74 cells in 4 mice (G-Flamp1). Scale bar, 50 μm. d, Averaged ΔF/F of cAMPinG1-ST, cAMPinG1mut-ST, and G-Flamp1 in response to forced running. n = 3 trials in 3 mice (cAMPinG1-ST), n = 4 trials in 4 mice (cAMPinG1mut-ST), n = 4 trials in 4 mice (G-Flamp1). Unpaired two-tailed t-test. cAMPinG1-ST vs cAMPinG1mut-ST: P = 2.6 × 10−2, cAMPinG1-ST vs G-Flamp1: P = 4.6 × 10−2. e, Schematic of the experimental procedure. cAMPinG1-NE was expressed in neurons in the V1. Fiber photometry was performed during forced running. Thirty minutes after intraperitoneal administration of propranolol, a β-adrenoceptor blocker, fiber photometry was again performed during the forced running. f, Averaged traces of cAMPinG1-NE in response to forced running before and after intraperitoneal administration of mock solution (left) or propranolol (right). n = 6 trials in 6 mice (mock), n = 6 trials in 6 mice (propranolol). g, Difference of ΔF/F in response to forced running before and after the intraperitoneal administration. n = 6 trials in 6 mice (mock), n = 6 trials in 6 mice (propranolol). Paired two-tailed t-test. P = 1.4 × 10−2. h, Schematic of the experimental procedure. i, Representative images of cAMPinG1 and RCaMP3. Scale bar, 50 μm. j, Averaged fluorescence transients of cAMPinG1 and RCaMP3. n = 63 cells in 3 mice. All shaded areas and error bars denote SEM.
Extended Data Fig. 7 In vivo Ca2+ and cAMP imaging during visual stimuli.
a,b, Representative two-photon images and traces of RCaMP3 and cAMPinG1-ST of the cells in Fig. 5b-c, indicated by arrows. Scale bar, 10 μm. c, Averaged traces of cAMPinG1-ST (left), cAMPinG1mut-ST (middle), and G-Flamp1 (right) in response to visual stimuli. n = 1156 cells in 3 mice (cAMPinG1-ST), n = 905 cells in 4 mice (cAMPinG1mut-ST), n = 74 cells in 4 mice (G-Flamp1). Four trials in each cell. d, Averaged ΔF/F of cAMPinG1-ST, cAMPinG1mut-ST, and G-Flamp1 in response to visual stimuli. n = 12 trials in 3 mice (cAMPinG1-ST), n = 16 trials in 4 mice (cAMPinG1mut-ST), n = 16 trials in 4 mice (G-Flamp1). Unpaired two-tailed t-test after removing outliers with Smirnov-Grubbs’ test (P = 0.05). cAMPinG1-ST vs cAMPinG1mut-ST: P = 1.9 × 10−5, cAMPinG1-ST vs G-Flamp1: P = 7.2 × 10−4. e, Averaged ΔF/F of RCaMP3 and cAMPinG1mut-ST in cells which showed Ca2+ response to visual stimuli. n = 486 cells in 4 mice. All shaded areas and error bars denote SEM.
Extended Data Fig. 8 Dual-color fiber photometry for Ca2+ and cAMP in dorsal striatum neurons.
a, Schematic of the experimental procedure. AAVs encoding RCaMP3 and green cAMP sensors were injected into the dorsal striatum (dStr). Dual-color fiber photometry was performed in the dStr during a forced running task. For dual-color imaging, we employed different excitation wavelengths: 560 nm for RCaMP3 imaging and 405 nm and 470 nm for cAMPinG1 ratiometric imaging. b, Representative single-trial traces of cAMPinG1-NE (left), cAMPinG1mut-NE (middle), G-Flamp1 (right), and RCaMP3 signals. c, Averaged fluorescence traces of cAMPinG1-NE (left), cAMPinG1mut-NE (middle), G-Flamp1 (right), and RCaMP3 signals. n = 9 trials in 1 mouse (cAMPinG1-NE), n = 9 trials in 1 mouse (cAMPinG1mut-NE), n = 9 trials in 1 mouse (G-Flamp1). d, Averaged ΔF/F of cAMPinG1-NE, cAMPinG1mut-NE and G-Flamp1 during and after the stimulation. n = 36 trials in 4 mice (cAMPinG1-NE), n = 27 trials in 3 mice (cAMPinG1mut-NE), n = 36 trials in 4 mice (G-Flamp1). Unpaired two-tailed t-test. cAMPinG1-NE vs cAMPinG1mut-NE (during stim): P = 3.7 × 10−4; cAMPinG1-NE vs G-Flamp1 (during stim): P = 3.6 × 10−5; cAMPinG1-NE vs cAMPinG1mut-NE (after stim): P = 3.1 × 10−5; cAMPinG1-NE vs G-Flamp1 (after stim): P = 4.2 × 10−5. All shaded areas and error bars denote SEM.
Extended Data Fig. 9 Timelapse and single-timepoint imaging of cAMPinG1.
a, Single timepoint imaging of cAMPinG1 (top) and G-Flamp1 (bottom) in the absence or presence of 10 μM forskolin. Scale bar, 20 μm. b, Single timepoint imaging of cAMPFIRE-L in the absence or presence of 10 μM forskolin. Scale bar, 20 μm. c, Relative change (ΔR/R) in fluorescence ratio (R) in the absence or presence of 10 μM forskolin. For cAMPinG1 and G-Flamp1, R is the ratio of green fluorescence with 488 nm excitation to that with 405 nm excitation. For cAMPFIRE-L, R is the ratio of cyan fluorescence with 458 nm excitation to yellow fluorescence with 514 nm excitation. n = 115 (cAMPinG1 without forskolin), 155 (cAMPinG1 with forskolin), 75 (G-Flamp1 without forskolin), 102 (G-Flamp1 with forskolin), 86 (cAMPFIRE-L without forskolin), 74 (cAMPFIRE-L with forskolin) cells. Tukey’s post hoc test following one-way ANOVA. cAMPinG1 vs G-Flamp1: P < 1.0 × 10−13; cAMPinG1 vs cAMPFIRE-L: P < 1.0 × 10−13; G-Flamp1 vs cAMPFIRE-L: P < 1.0 × 10−13. d, A representative image of the cAMPinG1 stable cell line. Scale bar, 20 μm. e, Proliferation assay of the cAMPinG1 stable cell line and original HEK293T cell line. The original HEK cell line in 1.5 % FBS condition was for a negative control of the assay. n = 6 wells in each cell line. Tukey’s post hoc test following one-way ANOVA. cAMPinG1 cell line in 10% FBS vs original cell line in 1.5% FBS: P = 1.9 × 10−5; Original cell line in 10% FBS vs original cell line in 1.5% FBS: P = 5.0 × 10−4. f, Single timepoint imaging of cAMPinG1 cell line in the absence (top) or presence (bottom) of 50 μM forskolin. Scale bar, 20 μm. Error bars denote SEM. Boxes indicate 25th and 75th percentiles, solid lines indicate median, dashed lines indicate mean, and whiskers indicate total range of data.
Extended Data Fig. 10 Triple stable cell line for drug screening.
a, Single-timepoint imaging of cAMPinG1, DRD1, and mCherry triple stable cell line in the absence (top) and presence (bottom) of 100 nM dopamine. Scale bar, 20 μm. b, Single-timepoint imaging of cAMPinG1, melanocortin 3 receptor (MC3R, a Gs-coupling GPCR) and iRFP670 triple stable cell line in the absence (top) or presence (bottom) of 1,000 nM ACTH. Scale bar, 20 μm. c, Schematic of the expression system (top). Representative images of a mixture of the cell lines with 100 nM dopamine (middle) or 1,000 nM ACTH (bottom). Representative cells expressing DRD1/RFP or MC3R/iRFP were indicated by arrows or arrowheads, respectively. Scale bar, 20 μm. d, 488 ex/405 ex ratio of each cell line in the presence of dopamine or ACTH. n = 95 (ACTH + DRD1), 87 (ACTH + MC3R), 72 (dopamine + DRD1), 161 (dopamine + MC3R) cells. Boxes indicate 25th and 75th percentiles, solid lines indicate median, dashed lines indicate mean, and whiskers indicate total range of data. Tukey’s post hoc test following one-way ANOVA. ACTH + DRD1 vs ACTH + MC3R: P = 2.4 × 10−11; ACTH + DRD1 vs dopamine + DRD1: P = 2.1 × 10−13. ACTH + MC3R vs dopamine + MC3R: P = 2.1 × 10−13; dopamine + DRD1 vs dopamine + MC3R: P = 2.1 × 10−13.
Supplementary information
Supplementary Information
Supplementary Figs. 1–3 and Supplementary Tables 1 and 2.
Supplementary Video 1
FASHIO-2PM for RCaMP3 imaging from L5 neurons in an awake condition. The size of the imaging area is 3.0 × 3.0 mm2 (2,048 × 2,048 pixels), acquired at 7.5 Hz. Images are filtered with Kalman stack filter by ImageJ.
Supplementary Video 2
Mouse was presented with moving grating in eight directions to the contralateral eye in awake condition. Sequential excitation at 940 nm and 1,040 nm was used for dual-color imaging of RCaMP3 and cAMPinG1-ST. Three optical planes spaced 30 μm apart were imaged at 3.4 Hz per plane using a piezo objective scanner. The size of the imaging area is 339 × 339 μm2 (512 × 512 pixels).
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Yokoyama, T., Manita, S., Uwamori, H. et al. A multicolor suite for deciphering population coding of calcium and cAMP in vivo. Nat Methods 21, 897–907 (2024). https://doi.org/10.1038/s41592-024-02222-9
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DOI: https://doi.org/10.1038/s41592-024-02222-9