Abstract
Two-component systems, consisting of a histidine kinase and a response regulator, serve signal transduction in bacteria, often regulating transcription in response to environmental stimuli. Here, we identify a tandem serine histidine kinase function for KdpD, previously described as a histidine kinase of the KdpDE two-component system, which controls production of the potassium pump KdpFABC. We show that KdpD additionally mediates an inhibitory serine phosphorylation of KdpFABC at high potassium levels, using not its C-terminal histidine kinase domain but an N-terminal atypical serine kinase domain. Sequence analysis of KdpDs from different species highlights that some KdpDs are much shorter than others. We show that, while Escherichia coli KdpD’s atypical serine kinase domain responds directly to potassium levels, a shorter version from Deinococcus geothermalis is controlled by second messenger cyclic di-AMP. Our findings add to the growing functional diversity of sensor kinases while simultaneously expanding the framework for regulatory mechanisms in bacterial potassium homeostasis.
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Introduction
K+ homeostasis is central to enabling microbial survival under changing conditions. In many prokaryotes, high-affinity K+ uptake at low extracellular K+ concentrations is facilitated by the active K+ pump KdpFABC1,2,3. Its chimeric composition of a K+ channel and a P-type ATPase allows the complex to accumulate K+ with an apparent affinity of 2 µM4. KdpFABC is particularly required under extreme K+ limitation, while at increased extracellular concentrations toxic amounts of K+ would be accumulated by its constant pumping2. Therefore, the expression of kdpFABC in Escherichia coli is controlled by the two-component system (TCS) KdpDE, which comprises the sensor histidine kinase (HK) KdpD and the transcription factor KdpE5 (Fig. 1). KdpD senses extra- and intracellular K+ levels6,7. At low K+ concentrations, auto-phosphorylated KdpD phosphorylates KdpE, which, upon dimerization, activates the transcription of kdpFABC8,9. When viable K+ conditions at which other transport systems can mediate K+ uptake are restored, KdpFABC is no longer needed. At these conditions, KdpD switches to a phosphatase activity, dephosphorylating KdpE to stop continued transcription of kdpFABC10,11.
Notably, the transcriptional control by KdpDE does not address KdpFABC complexes already in the membrane. If left unregulated, these would continue to excessively accumulate K+2. To prevent this, KdpFABC is inhibited at K+ concentrations above 2 mM via a phosphorylation of residue S162 of the P-type ATPase KdpB12,13,14,15 (All residue numbers refer to E. coli KdpD and KdpFABC unless otherwise specified). This process appears to be irreversible, and prevents KdpFABC from progressing through its normal catalytic cycle14,15. Full inhibition was observed in situ two minutes after exposure to K+12. However, the source of this phosphorylation is yet to be determined.
While phosphorylation is best characterized in eukaryotes, bacterial protein kinases have increasingly come into focus. They can be separated into five categories: eukaryotic-like serine/threonine kinases (eSTKs or Hanks kinases), arginine kinases, HKs, bacterial tyrosine kinases (BYKs), and atypical serine kinases (ASKs)16. eSTKs have the same characteristic motifs described for eukaryotic serine/threonine kinases and have been found to control metabolism in a wide range of bacterial species, but not in E. coli17,18,19. Arginine kinases have been implicated in control of bacterial protein degradation20. HKs are sensors that autophosphorylate at a histidine, from where the phosphate is transferred to an aspartate on a response regulator to regulate gene expression21. BYKs catalyze the phosphorylation of tyrosines via three Walker motifs, which are canonically involved in ATPase activities but here function as kinase domains22. The same active site, albeit only containing a Walker A and a Walker B motif, is found in ASKs16. Members of this family regulate diverse metabolic processes by serine phosphorylation23,24. Notably, a large number of known bacterial phosphorylation targets still lack an identified kinase25,26.
Previously proposed mechanisms for KdpBS162 phosphorylation include autocatalysis by KdpFABC or an unknown serine kinase that could be activated, for instance, by HK KdpD, whose low/high K+ switch corresponds exactly to the concentration range at which KdpFABC inhibition occurs12,14. Here, we show that KdpD in fact itself acts as a tandem serine histidine kinase that both controls the transcription of kdpFABC and directly phosphorylates KdpB for post-translational inhibition. By analyzing the inhibition of KdpFABC in the presence of different KdpD constructs in vivo and in vitro, we show that the Walker A/B motif of the N-terminal KdpD domain functions as an ASK that phosphorylates KdpBS162 and thus inactivates KdpFABC at high K+ conditions. By further analyzing the diversity of the domain organization of KdpD across prokaryotic species, we found that the ASK is actually more conserved than the HK and therefore may be the most determinant function of KdpD, and that the ASK activity of KdpDs from different species is controlled either directly by the K+ concentration or by the binding of the second messenger c-di-AMP.
Results
KdpD mediates the phosphorylation of KdpBS162
The inhibition of KdpFABC via KdpBS162 phosphorylation at high external K+ concentrations is essential to prevent K+ toxicity2,14. Here, we set out to identify players responsible for the regulatory phosphorylation of KdpFABC. To characterize the inhibition level of KdpFABC, we tested the rate of ATP hydrolysis in an ATPase assay and the phosphorylation level of KdpB using a phosphoprotein gel stain (Fig. 2, Supplementary Fig. 1). KdpFABC was produced at high K+ conditions in E. coli LB2003 cells, which lack kdpFABC but still express kdpDE, or in E. coli TK2281 cells, in which the entire kdpFABCDE operon is deleted. KdpFABC purified from E. coli LB2003 cells showed a low residual ATPase activity and a high level of KdpB phosphorylation, as previously reported27,28. When the inhibitory phosphorylation was prevented by mutation KdpBS162A13, ATP hydrolysis was increased six-fold, and no phosphorylation was observed. To rule out an auto-catalyzed phosphorylation of KdpBS162, ATP binding to KdpFABC was prevented by point mutations KdpBF377A/K395A29. The resulting construct showed no ATPase activity but retained the phosphorylation of KdpB, indicating that KdpBS162 phosphorylation is mediated by a separate kinase.
It was hypothesized that KdpD, which regulates the transcription of kdpFABC, could also control a kinase that phosphorylates KdpBS162. To test this hypothesis, we purified KdpFABC from the ∆kdpDE E. coli strain TK2281 grown at high K+. KdpFABC produced under these conditions showed a high ATPase activity and no phosphorylation of KdpB, indicating that the inhibition was lifted (Fig. 2). Thus, KdpDE must be involved in KdpFABC inhibition. We considered three possible mechanisms for the regulation: through a separate kinase activated by KdpD, through a direct phosphorylation by KdpD, or through a separate kinase activated by KdpE. To distinguish the latter option from the first two, KdpD was individually reintroduced in E. coli TK2281 cells via expression from an orthogonal plasmid. Successful production of KdpD constructs was verified by in-gel fluorescence of whole-cell samples (Supplementary Fig. 2). KdpFABC purified from these cells was again inhibited, with an even lower ATPase activity than samples purified from E. coli LB2003 cells, and, coherently, showed a high phosphorylation level of KdpB. The stronger inhibition likely stems from the over-expression of KdpD compared to the low expression under the native promoter in E. coli LB2003 cells12,14,30. Thus, KdpBS162 phosphorylation appears to be solely dependent on KdpD, and does not require the response regulator KdpE.
To elucidate the mechanism by which the phosphorylation is carried out, directly or indirectly, we next investigated the role of the individual domains of KdpD, focusing on those that potentially could mediate a phospho-transfer directly. The basic requirements for a phospho-transfer are proximity of the nucleotide or phospho-intermediate and the side chain that needs to be phosphorylated, their polarization, and the exclusion of water. KdpD consists of a four-helix transmembrane (TM) domain with two large cytosolic segments N- and C-terminally (Fig. 2 b). The N terminus comprises the KdpD domain followed by a Usp (universal stress protein) domain, while the C terminus harbors a GAF (cGMP-specific phosphodiesterases, adenylyl cyclases and FhlA) domain followed by a transmitter domain. Of these domains, the KdpD domain and the transmitter domain contain ATP binding sites that could serve the phospho-transfer to KdpB. The transmitter domain comprises a dimerization and histidine phospho-transfer (DHp) domain, and a catalytic and ATP-binding (CA) domain, and is conserved among HKs21. It forms the catalytic core of the HK: the CA domain binds ATP, positioning it for autophosphorylation of a histidine in the DHp domain. In E. coli KdpD, H673 is phosphorylated at low K+. Subsequently, the phospho-histidine intermediate transfers its phosphate to KdpE11. The KdpD domain on the other hand is highly conserved among KdpDs from different species but not found in any other sensor kinase31. ATP binding to the Walker A-like motif in the KdpD domain has been implicated in regulating the phosphatase activity of KdpD at high K+32. This motif diverges from the classical Walker A sequence by one extra residue (G-X5-GKT instead of G-X4-GKT)33. Additionally, the KdpD domain also contains a Walker B motif31.
To test whether the phospho-histidine intermediate is used to phosphorylate KdpBS162, the catalytic phospho-histidine was precluded by the mutation KdpDH673A (Fig. 2). KdpFABC produced in this background retained the inhibition and phosphorylation, indicating that the phospho-histidine is not involved in KdpBS162 phosphorylation.
Alternatively, the two ATP-binding sites, the HK catalytic site in the CA domain or the Walker motifs in the N-terminal KdpD domain, could directly transfer the phosphate from ATP to KdpB. To test the role of these two sites, ATP binding was individually abolished by deleting the catalytic DHp and CA domains (KdpD∆663-894), referred to as KdpD∆DHpCA, or by introducing the mutations KdpDG36A/K37A/T38C in the Walker A motif, referred to as KdpD∆WalkerA32. The deletion of the catalytic DHp and CA domains showed no effect on KdpFABC inhibition; ATPase activity remained low and KdpB showed high phosphorylation (Fig. 2). Mutating the Walker A motif in the KdpD domain, on the other hand, abolished the inhibition of KdpFABC and the phosphorylation of KdpBS162. Thus, the Walker motifs in the KdpD domain appear to be essential for the inhibitory phosphorylation.
KdpBS162 phosphorylation is mediated by KdpD’s N-terminal domain in an atypical serine kinase mechanism
While the co-expression studies in vivo indicate an important role of KdpD in KdpFABC inhibition, it is not possible to exclude a separate intermediary between the sensor kinase and KdpB. To show that the phosphorylation occurs directly between the KdpD domain and KdpB, KdpFABC and KdpD variants were purified separately from E. coli TK2281 cells (Supplementary Fig. 3) and mixed in vitro in the presence of 400 mM KCl and 5 mM ATP to stimulate the serine kinase activity. All KdpFABC constructs featured the mutation KdpBD307N to preclude the catalytic phosphorylation of the P domain. KdpB phosphorylation was analyzed and quantified by phosphoprotein gel stain (Fig. 3 a, Supplementary Fig. 4). Without KdpD, no phosphorylation was observable. Upon incubation with full-length KdpD, the phosphorylation was fully restored. KdpFABS162A/D307NC mixed with full-length KdpD showed no phosphorylation for KdpB, indicating that the signal was specific for KdpBS162 phosphorylation (Supplementary Fig. 4). Thus, phospho-transfer must occur directly from KdpD to KdpB, making KdpD a tandem serine histidine kinase.
To show that the N-terminal KdpD domain itself catalyzes the phosphorylation, a truncated construct with only this domain (KdpD1-230, denoted as KdpDNTD) was purified and incubated with KdpFABC. This truncated construct remained able to phosphorylate KdpB, indicating that the domain acts as a functional module within the sensor kinase that can by itself carry out a kinase activity. In fact, phosphorylation by KdpDNTD was stronger than by full-length KdpD when applied at equimolar concentrations, which could suggest that the other domains of KdpD modulate the serine kinase activity. Alternatively, it is also possible that the soluble domain more easily interacts with KdpB in vitro, while full-length KdpD is hampered by a clash of the two detergent micelles.
From sequence analysis and an AlphaFold structural prediction of the complex between KdpDNTD and the KdpB A domain34,35, three conserved common ATP-binding motifs, characteristic for an ASK, are apparent: Walker A and Walker B motifs for coordination of the β- and γ-phosphates, and a tryptophan/arginine pair (KdpDW39/R190) for the coordination of the adenine (Fig. 3b, c; Supplementary Fig. 5). Mutating the Walker A motif or the adenine coordination motif (KdpD∆WalkerA or KdpDW39A/R190A, respectively) almost completely abolished phosphorylation of KdpB (Fig. 3), likely by preventing ATP binding. Whereas the Walker A motif is essential for ATP binding, the Walker B motif is responsible for catalysis of ATP hydrolysis in Walker A/B-containing ATPases36,37. More precisely, the aspartate of the Walker B motif polarizes the gamma phosphate via coordination of a Mg2+, while the glutamate polarizes a water molecule for hydrolysis; mutation of these residues in ATPases does not abolish ATP binding but prevents hydrolysis37. In the interaction model of KdpDNTD and the KdpB A domain, the Walker B motif (KdpDD109/E110) is positioned ideally for a polarizing function in the serine kinase mechanism, with KdpDE110 activating KdpBS162 rather than a water. Indeed, mutating the residues of the Walker B motif individually (KdpDD109A and KdpDE110A) abolished the phosphorylation of KdpB, indicating that both are essential for the phosphorylation mechanism. Interestingly, glutamate KdpBE161 is positioned behind KdpDE110 of the Walker B motif in the complex model, suggesting a supporting role in the polarization. Indeed, mutation KdpBE161Q resulted in a reduction in phosphorylation, but only by 64%. Based on its position behind KdpDE110, it appears that KdpBE161 potentiates the polarization of KdpBS162 by the Walker B motif. These results suggest that KdpBS162 directly attacks the γ-phosphate in a mechanism reminiscent of other ASKs, while the Walker B motif functions as in ATPases, with KdpDE110 activating the serine for the nucleophilic attack, supported by KdpBE161 (Fig. 3d).
To test the specificity of the ASK-type serine phosphorylation, KdpBS162 was mutated to threonine and tyrosine, both of which resulted in no phosphorylation (Fig. 3 a). The identical observation for KdpBS162Y and KdpBS162T suggests that the effect is most likely due to active site geometry, in which only serine is suited for polarization by KdpDE110 and KdpBE161.
Further, we tested KdpDNTD for autophosphorylation and ATPase activity (Supplementary Fig. 6). In agreement with an ASK mechanism, in which neither of these occur, KdpDNTD is not phosphorylated under any conditions, even under non-steady-state conditions where phospho-transfer to KdpB is prevented by the mutation KdpBS162A, and showed no ATPase activity, indicating that the Walker B motif does not exert a hydrolysis function as in Walker A/B-containing ATPases36,37. These results align with the mechanism deduced from the mutagenesis studies, supporting the characterization of the KdpD domain as an ASK.
KdpD’s ASK activity is conserved across species but distinctly regulated
After identifying the ASK activity of the KdpD domain in E. coli KdpD, we wondered how conserved this regulatory mechanism was across prokaryotic species. To investigate this, we analyzed the sequences of KdpD from 5495 species that feature an annotation for the Kdp pump in the UniProt database (Supplementary Data 1, 2). The dataset was initially based on that of a previous study38, which was subsequently expanded to include all species from the LPSN database fitting the parameters39. KdpD sequences were classified according to their domain architecture, with particular emphasis on the presence of the KdpD and DHpCA domains, which confer the ASK and HK activities, respectively (Fig. 4a). Notably, this dataset is dependent on annotations and sequences in the Uniprot database. While faulty annotations were removed by sequence alignments, incomplete sequences cannot be excluded. However, the frequency of the five most common classes of KdpD in the dataset suggests that a sequencing artifact is, in these cases, highly unlikely. While almost 75% of species contain a ‘full-length’ KdpD version, featuring all the domains present in E. coli KdpD, a significant fraction deviates from this architecture, with the most significant versions shown in Fig. 4b and the distributions for individual phyla shown in Supplementary Fig. 7a, b. Some species feature other KdpD variants in addition to a ‘full-length’ version; in these cases, only the full-length variant was included in the analysis, as it fulfills the requirements for both HK and ASK activities. Interestingly, some species contain the ASK and HK activities on separate polypeptide chains, with the most common combination consisting of a KdpD/Usp domain ASK and a TM/GAF/DHpCA domain HK. Moreover, there are multiple versions of KdpD in which the requirements for the HK are not fulfilled but the ASK domain is conserved. These can be separated into versions consisting of only the N-terminal KdpD and Usp domains, or versions in which the C-terminal DHpCA domains are truncated, removing the HK activity. In these species, it appears that the sole role of KdpD is the post-translational regulation of KdpFABC via the ASK-mediated phosphorylation. Indeed, KdpD versions lacking the domains for HK activity are predominantly found in species also missing the transcription factor KdpE; they make up almost 90% of KdpD versions found in these species. By contrast, species containing KdpE overwhelmingly feature KdpD versions or combinations that mediate a HK activity for transcriptional control in addition to the ASK activity (95%; Fig. 4c). Importantly, in almost all versions, the KdpD domain with the ASK function was preserved.
The shortened KdpD/Usp domain version poses an interesting conundrum, as all previously suggested K+ sensing sites in E. coli KdpD (EcKdpD), albeit only characterized for the HK, are located in the TM/GAF domains7. To investigate how post-translational regulation by KdpD is linked to K+ levels in these species, we compared the stimulation of ASK activity between E. coli KdpD, the well-studied full-length chain, and a shorter KdpD/Usp domain version from Deinococcus geothermalis (DgKdpD, UniProt accession Q1J2K3) (Fig. 5a). In vivo, the phosphorylation of KdpBS162 in E. coli and the HK and phosphatase activities of KdpD are dependent on K+ levels12,14. However, when purified in DDM, the HK activity is constantly low, while the phosphatase activity is high10. In agreement with this observation, KdpD solubilized in DDM here showed a constantly high serine kinase activity (Fig. 5b). Since a membrane environment was shown to restore the K+ dependence of KdpD’s HK activity10, we solubilized KdpD directly from E. coli membranes in SMALPs to retain the K+ stimulation of the serine kinase activity. Contrary to detergents like DDM, which displace lipids to form micelles tightly around the TM domain, SMALPs can form stable lipid discs containing the membrane protein. Indeed, KdpD’s ASK activity in SMALPs was stimulated upon the addition of K+, suggesting that the N-terminal domain also underlies the control of the K+-sensing domains of KdpD (Fig. 5b). Conversely, DgKdpD’s ASK activity is not stimulated by K+ (Fig. 5c). In Staphylococcus aureus KdpD, the Usp domain was reported to bind c-di-AMP, a second messenger, which in general has been implicated in a wide range of osmotic and K+ homeostasis regulation mechanisms40. Binding of c-di-AMP led to the downregulation of kdpFABC expression41,42. Consequently, we suspected that c-di-AMP binding to the Usp domain could be the signal for ASK stimulation in KdpD versions lacking K+ sensing domains. Indeed, phosphorylation of E. coli KdpB by DgKdpD was stimulated in the presence of c-di-AMP, indicating that, here, the K+-sensing role of KdpD is outsourced to the diadenylate cyclase that forms c-di-AMP at increased K+ levels43. In agreement with this hypothesis, the majority of species featuring a shorter KdpD/Usp domain architecture also express a diadenylate cyclase (Fig. 4d). At the same time, the fact that DgKdpD can phosphorylate E. coli KdpB shows that the regulatory phosphorylation is conserved, independent of the input domains.
Discussion
The regulation of KdpFABC by phosphorylation of KdpBS162 is an essential process in facilitating microbial adaptability to changing K+ conditions. Here, we show that the rapid, irreversible inhibition of KdpFABC at high K+ is widely conserved and mediated directly by a Walker A/B motif in the N-terminal KdpD domain of KdpD in an atypical serine kinase mechanism, making KdpD a tandem serine histidine kinase. Moreover, we identified a shorter form of KdpD without HK domains in some species that only fulfills the role of post-translational control of KdpFABC, regulated by the second messenger c-di-AMP.
Physiologically, the use of KdpD to also inhibit KdpFABC post-translationally in E. coli is remarkably smart since, this way, two outcomes, HK and ASK activities, are controlled by the same input: K+ sensing by the tandem serine histidine kinase. Thus, KdpD acts as a single, centralized regulator for both KdpFABC activity and gene expression to ensure its required strict control6,12: At low K+ conditions, KdpD’s HK activity is stimulated to phosphorylate KdpED52 via a phospho-histidine intermediate in the DHp domain, causing KdpE to dimerize and activate kdpFABC transcription5. The activation of the histidine kinase is further supported by the binding of UspC to the Usp domain44 (Fig. 6a). Conversely, at moderate to high K+ concentrations, K+ binds to KdpD’s sensing domains in the TM (extracellular) or GAF domains (intracellular), switching the DHp domain from its histidine kinase to its phosphatase activity and mediating the dephosphorylation of KdpE6,7,8,9 (Fig. 6b). Simultaneously, at these conditions, the N-terminal KdpD domain binds ATP32, and, as we now know, phosphorylates KdpBS162 as an ASK to inhibit the pump on a post-translational level to prevent excessive K+ uptake. Specificity for the reaction is probably provided by KdpBE161, which is required for the polarization of KdpBS162. This could actually explain how the ASK prevents unspecific hydrolysis of ATP by the Walker A/B motif that, in other ATPases, is able to independently hydrolyze ATP: only in the complex with the substrate is the polarization by the Walker B motif of KdpD strong enough to facilitate ATP cleavage.
The direct phospho-transfer from KdpD to KdpBS162 presented here coincides with multiple previously reported observations: KdpD was shown to interact with KdpB, but for an unknown reason45,46. Both KdpFABC and KdpD are also regulated by cardiolipin, suggesting a possible co-localization in the membrane15,47,48. Moreover, the KdpD domain containing the Walker A/B motif is highly conserved among KdpDs from different species, but not found in other sensor kinases31. Thus, it provides an ideal platform for a specific interaction with KdpB, which is a prerequisite for a precise and selective regulatory function.
Species featuring a short KdpD with only the N-terminal KdpD and Usp domains, like DgKdpD, appear to employ a more centralized regulation: post-translational control of KdpFABC by c-di-AMP binding to KdpD (Fig. 6c, d). At low K+ levels, the concentration of c-di-AMP is low43; consequently, KdpD is inactive. At high K+ conditions, a diadenylate cyclase forms c-di-AMP, which binds to the Usp domain of KdpD and activates the ASK activity of the NTD to inhibit KdpFABC. In addition to the post-translational regulation, some species of the Bacillota phylum also use c-di-AMP for the transcriptional control of kdpFABC via a c-di-AMP-responsive ydaO riboswitch, putting the entire Kdp system under the control of the second messenger38. The full control of some Kdp systems by c-di-AMP is reminiscent of the regulation of K+ transport systems KtrAB and KimA in Bacillus subtilis, whose expression is controlled by c-di-AMP binding to the ydaO riboswitch and which are additionally allosterically regulated by the second messenger43,49,50,51,52,53. This works highlights another role for c‑di-AMP in K+ homeostasis, emphasizing the importance of the second messenger in bacterial adaptability. But what about organisms featuring neither ‘full-length’ KdpD nor a riboswitch? In some species such as Deinococcus radiodurans, Anabaena sp., and Alicyclobacillus acidocaldarius, kdp expression is regulated by a separate TCS54,55,56. Interestingly, in these species, the ASK‑containing chain is retained downstream of the pump in the kdp operon, while the HK is separate. This indicates that the read-through expression of the ASK is important to ensure sufficient protein levels to efficiently inhibit the pump when required. In other organisms, no separate HK was identified, and the expression of kdpFABC could be constitutive or controlled by another unknown mechanism.
What remains to be addressed is why KdpD is controlled by different inputs and whether one is superior over the other. The regulation by K+ levels could allow for a more independent control of KdpFABC from other K+ transport systems. However, c-di-AMP binding sites from different proteins show large structural differences, so a graded control of K+ transport systems could also be facilitated by different affinities for c-di-AMP. A particularly intriguing question is how KdpDs that harbor both the K+ sensing domains and the c-di-AMP binding site, like for example S. aureus KdpD41, are regulated. Are the different stimuli additive or is one dominant over the other? Or are the different functions of KdpD controlled separately, i.e. is the ASK activity controlled by c-di-AMP and the HK activity by K+ sensing? In EcKdpD, multiple signals regulate the HK activity, including the two K+ sensing domains and UspC binding in the Usp domain. The integration of multiple signals bears the advantage of an even more differentiated regulation of the sensor kinase. However, control of the ASK activity remains to be further characterized
What generally surprised us after sequence analysis of KdpDs from different species was the high variability in the types of KdpD found, even if the dataset is limited by the number of sequenced genomes and the annotation of sequences in the UniProt database. The distribution of KdpD versions in different phyla makes speculation about the evolution of the KdpD(E) regulatory system enticing (Supplementary Fig. 7). In some species, most commonly in Acidobacteriota and Chloroflexota, ASK and HK activities are separated on two polypeptide chains, suggesting that these functions evolved separately with distinct regulatory mechanisms. The HK chains contain the domains known to be K+ sensors in E. coli KdpD, suggesting that the HK is always controlled by K+. In these species, the shorter ASK KdpD is controlled by the Usp domain. The phyla featuring mainly this short KdpD version (Bacteroidota, Cyanobacteriota, Deinococcota, Myxococcota) mostly contain diadenylate cyclases, suggesting that control by c-di-AMP, as shown here for D. geothermalis KdpD, could be a common theme. The ASK and HK chains must have at some point been combined into the E. coli-like ‘full-length’ chain by gene fusion, after which ASK activity came under the control of the K+-sensing domains. This is supported by the fact that some ‘full-length’ KdpDs still bind c-di-AMP in their Usp domain41,57. Fascinatingly, some phyla (Campylobacterota, Archaea) appear to have evolved back to lose the HK activity, featuring a truncated DHpCA domain. These phyla are often found in extreme conditions, where kdpFABC expression may be constantly needed, making transcriptional control superfluous. Accordingly, a significant fraction of Archaea completely lacks any form of KdpD. The phylum with the highest KdpD variance is Bacillota, probably due to their natural competence, which allowed them to scavenge kdpD genes from different sources in their surroundings58.
Finally, we would like to place the tandem serine histidine kinase activity of KdpD into the context of non-canonical functions of other sensor kinase systems. Canonically, two-component systems transduce environmental signals downstream to induce a physiological response on the transcriptional level. However, recently, this view of sensor kinases as a linear signaling pathway has shifted, as multiple cases of transporter-kinase interactions have been characterized59,60,61. In these systems, the cognate transporter interacts with the sensor kinase to convey additional stimuli. The additional regulation can range from feedback control on transcription levels60 to detection of transport activity62,63,64 or even sensing of stimuli by the transporter65. However, in all of the described systems, the interactions serve to provide additional stimuli to the kinase in a form of extended feedback regulation. The regulation of KdpFABC by KdpD additionally shows post-translational control of a transporter by its sensor kinase. However, the post-translational regulatory role could be more widespread, as the presence of Walker A/B motifs in a cytosolic domain has been described for other histidine kinases66,67.
The data presented here shows that the inhibition of KdpFABC is mediated by an atypical serine kinase domain in the N terminus of KdpD, completing the puzzle of how cells tightly regulate K+ uptake by KdpFABC to ensure robust K+ homeostasis in rapidly changing conditions. Further work will be required to elucidate the exact sensing and intramolecular signal transduction mechanisms that allow KdpD to dynamically regulate its serine kinase activity. The presence of an atypical serine kinase domain on a histidine kinase opens up new possibilities in the search for bacterial kinases: domains previously thought to be ATPases or regulatory nucleotide binding sites may have unrecognized kinase functions, helping to explain how the diverse phosphorylation patterns in bacteria can be facilitated.
Methods
Cloning and protein production
Escherichia coli kdpFABC, kdpD, and kdpDNTD and Deinococcus geothermalis kdpD were cloned into the expression vector pBXC3H by FX cloning68. Point mutations based on these constructs were generated by site-directed mutagenesis69. To clone E. coli kdpD or kdpD∆DHpCA into pBAD33, the gene or gene fragment was amplified from genomic DNA with 5’ and 3’ restriction sites for XbaI and PstI, respectively. The purified PCR product and pBAD33 were digested with XbaI and PstI according to the manufacturer’s instructions (Thermo Fisher Scientific; Waltham, MA), and the resulting fragments mixed in a molar ratio of 5:1 for ligation with T4 DNA ligase according to the manufacturer’s instructions (New England Biolabs; Ipswich, MA). Point mutations based on these constructs were generated by site-directed mutagenesis. For cloning of GFP-tagged kdpD constructs in pBAD33, pBAD33 with the untagged kdpD gene was amplified with primers binding at the 3’ end of the ORF, omitting the stop codon and containing a PstI or HindIII (reverse/forward primer, respectively) restriction site. sfGFP was amplified with 5’ PstI and 3’ HindIII restriction sites. PCR products were digested with PstI and HindIII (Thermo Fisher Scientific; Waltham, MA) before ligation with T4 DNA ligase. All kdpFABC and kdpD constructs used are listed in Supplementary Table 1 and primers used are listed in Supplementary Table 2.
KdpFABC variants were produced in E. coli LB2003 (F- thi metE rpsL gaI rha kup1 ∆kdpFABC5 ∆trkA)70 or TK2281 (ΔkdpFABCDE trkA405 trkD1 nagA thi rha lacZ)71 cells transformed with the respective pBXC3H derivatives. For co-expression with E. coli kdpD constructs, cells were co-transformed with an orthogonal pBAD33 derivative encoding the KdpD variant. Cells were grown in 4 l KML with 100 µg/ml ampicillin (and 30 µg/ml chloramphenicol when co-transformed with pBAD33 derivatives). Cultures were inoculated to an OD600 of 0.1 and induced with 0.002% L-arabinose at OD600 1.3 for one hour at 37 °C.
E. coli KdpD variants were produced in E. coli TK2281 cells. Cells transformed with the respective pBXC3H derivatives encoding the KdpD variants were grown in 6 l KML with 100 µg/ml ampicillin. Cultures were inoculated to an OD600 of 0.1 and induced with 0.004% L-arabinose at OD600 0.9 for one hour at 37 °C.
D. geothermalis KdpD variants were produced in E. coli TK2281 cells. Cells transformed with the respective pBXC3H derivatives encoding the KdpD variants were grown in 12 l KML with 100 µg/ml ampicillin. Cultures were inoculated to an OD600 of 0.1, grown to an OD600 of 0.7 at 37 °C, and induced with 0.1% L-arabinose at 30 °C for two hours.
Bacterial strains and plasmids are available from the Hänelt group upon request.
KdpFABC purification
KdpFABC variants were purified as previously described, omitting size exclusion chromatography after anion exchange chromatography27,28. In short, after cell disruption using a Stansted cell disruptor (Homogenising Systems Ltd.; Essex, U.K.), membranes were harvested and incubated with 1% (w/v) DDM overnight. Unsolubilized proteins were removed by centrifugation at 150,000 × g for 30 min, and the supernatant incubated with 2 ml Ni2+-NTA sepharose pre-equilibrated with KdpFABC Ni-NTA buffer (50 mM Tris-HCl pH 7.5, 20 mM MgCl2, 150 mM NaCl, 10% glycerol, 0.025% w/v DDM) and 10 mM imidazole for 1 h. After washing with 25 column volumes of KdpFABC Ni-NTA buffer at an increased imidazole concentration of 50 mM, the protein-bound Ni2+-NTA was subjected to an additional low-salt washing step of 25 column volumes (10 mM Tris-HCl pH 7.5, 10 mM MgCl2, 10 mM NaCl, 50 mM imidazole, 0.025% w/v DDM), before elution with a low-salt imidazole elution buffer (10 mM Tris-HCl pH 7.5, 10 mM MgCl2, 10 mM NaCl, 250 mM imidazole, 0.025% w/v DDM). Elution fractions were subjected to AIEX using AIEX buffer (10 mM Tris-HCl pH 8.0, 10 mM MgCl2, 10 mM NaCl) and a HiTrap Q HP column (Cytiva; Marlborough, MA, USA) and eluted with a NaCl gradient from 10-500 mM.
E. coli KdpD purification
KdpD variants were purified largely as previously described10. In short, after cell disruption using a Stansted cell disruptor (Homogenising Systems Ltd.; Essex, U.K.), membranes were harvested and washed twice in a low-salt EDTA buffer (1 mM Tris-HCl pH 7.5, 3 mM EDTA). 10 ml aliquots of membranes set to a total protein concentration of 10 mg/ml were supplemented with 600 mM NaCl and 2% (w/v) LDAO and incubated for 30 min at 4 °C. Unsolubilized proteins were removed by centrifugation at 150,000 × g for 45 min, and the supernatant incubated with 2 ml Ni2+-NTA sepharose pre-equilibrated with KdpD Ni-NTA buffer (50 mM Tris-HCl pH 7.5, 500 mM NaCl, 10% glycerol, 10 mM β-mercaptoethanol, 0.04% w/v DDM) and 10 mM imidazole for 30 min. After washing with 25 column volumes KdpD Ni-NTA buffer at an increased imidazole concentration of 50 mM, the protein-bound Ni2+-NTA was subjected to an additional low-salt washing step of 25 column volumes (50 mM Tris-HCl pH 7.5, 10% v/v glycerol, 10 mM β-mercaptoethanol, 50 mM imidazole, 0.04% w/v DDM), before elution with a low-salt imidazole elution buffer (50 mM Tris-HCl pH 7.5, 10% v/v glycerol, 10 mM β-mercaptoethanol, 400 mM imidazole, 0.04% w/v DDM). Elution fractions were subjected to AIEX using using AIEX buffer (50 mM Tris-HCl pH 7.5, 10 mM β-mercaptoethanol, 0.04% w/v DDM) and a HiTrap Q HP column (Cytiva; Marlborough, MA, USA) and eluted with a NaCl gradient from 0 to 800 mM.
KdpDNTD was purified in the same manner, incubating the cytosol with Ni2+-NTA and omitting detergent from all buffers.
For purification of KdpD in SMALPs, KdpD-containing membranes were prepared as described above. 10 ml aliquots of membranes set to a total protein concentration of 10 mg/ml were supplemented with 4% (w/v) SMALP 140 (Orbiscope; Geleen, the Netherlands), set to a total volume of 20 ml, and incubated for 18 h at 4 °C. Unsolubilized proteins were removed by centrifugation at 150,000 × g for 45 min, and the supernatant diluted tenfold with SMALP buffer (50 mM Tris-HCl pH 7.5, 10 mM β-mercaptoethanol) supplemented with 500 mM NaCl before being incubated with 2 ml Ni2+-NTA sepharose pre-equilibrated with KdpD Ni-NTA buffer, omitting DDM, and 10 mM imidazole for 18 h. After washing with 25 column volumes KdpD Ni-NTA buffer, omitting DDM, at an increased imidazole concentration of 50 mM, the protein-bound Ni2+-NTA was subjected to an additional low-salt washing step of 25 column volumes (50 mM Tris-HCl pH 7.5, 10 mM β-mercaptoethanol, 50 mM imidazole), before elution with a low-salt imidazole elution buffer (50 mM Tris-HCl pH 7.5, 10 mM β-mercaptoethanol, 400 mM imidazole). Elution fractions were subjected to SDS-PAGE to determine fractions containing KdpD, which were subsequently pooled and buffer exchanged to SMALP buffer with a ZebaSpinTM 7 K MWCO desalting column (ThermoFisher Scientific, Waltham, MA).
D. geothermalis KdpD purification
D. geothermalis KdpD was purified largely like E. coli KdpDNTD, omitting the final AIEX chromatography step. In short, after cell disruption using a Stansted cell disruptor (Homogenising Systems Ltd.; Essex, U.K.), the cytosol was supplemented with 10 mM imidazole and 500 mM NaCl and incubated with 6 ml pre-equilibrated Ni2+-NTA sepharose for 1 h. After washing with 50 column volumes of wash buffer (50 mM Tris-HCl pH 7.5, 500 mM NaCl, 5% w/v glycerol, 10 mM β-mercaptoethanol, 50 mM imidazole), the column was incubated for 1 h with 1 mg/ml 3 C protease in elution buffer (50 mM Tris-HCl pH 7.5, 300 mM NaCl, 10% w/v glycerol, 10 mM β-mercaptoethanol) under light stirring before elution with elution buffer. Imidazole was removed from elution fractions by buffer exchange using a ZebaSpinTM 7 K MWCO desalting column (ThermoFisher Scientific, Waltham, MA).
ATPase assay
ATP hydrolysis by purified KdpFABC variants was tested by malachite green ATPase assay72. In brief, each reaction contained 2 mM ATP and 1 mM KCl. The reaction was started by adding 0.5–1.0 µg protein, and carried out for 5 min at 37 °C.
In vitro phosphorylation
To show phospho-transfer between KdpD and KdpFABC in vitro, a three-fold molar excess of purified KdpD was mixed with KdpFABC variants purified from E. coli TK2281 cells (i.e., without prior KdpBS162 phosphorylation) and 400 mM KCl. All KdpFABC variants used featured the mutation KdpBD307N to preclude the catalytic phosphorylation of the P domain. The reaction was started by the addition of 5 mM ATP, and incubated for 30 min at room temperature, after which the reaction was stopped by mixing with SDS sample buffer for subsequent SDS-PAGE and phosphoprotein gel stain.
To test the phosphorylation state of KdpDNTD, the same protocol was performed in the absence and presence of 5 mM ATP, and in the presence of KdpFABD307NC or KdpFABS162AC/D307N in a 3:1 molar ratio KdpDNTD:KdpFABC.
To test the stimulation of E. coli or D. geothermalis KdpD serine kinase activity, the same protocol was performed in the absence and presence of 400 mM KCl or 1 mM c-di-AMP.
Phosphoprotein gel stain
For phosphoprotein gel staining, purified protein samples were separated by 12.5% SDS-PAGE73. Phosphorylated proteins were stained by Pro-Q™ Diamond Phosphoprotein Gel Stain (ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s instructions. Gels were subsequently stained with Coomassie brilliant blue R-250 to visualize the entirety of the loaded protein.
Sequence analysis
KdpD sequences and UniProt accession numbers from species analyzed in a previous study38 or included in the LPSN database39 were extracted and analyzed for homology to E. coli KdpD, the presence of the KdpD domain Walker A and B motifs, and the presence of the HK catalytic histidine by pairwise alignment using Clustal Omega74, following which they were manually classified based on domain structure and potential kinase functionality. For each species, the UniProt accession number for KdpE and a diadenylate cyclase was likewise extracted.
Expression test
To control expression of kdpD constructs from pBAD33 derivatives in co-expression experiments, GFP-tagged variants of KdpD were produced in E. coli TK2281 cells co-transformed with pBXC3H-kdpFABC. Samples from each culture were set to an OD600 of 30 in SDS sample buffer, and subjected to 12.5% SDS-PAGE with subsequent analysis of in-gel GFP fluorescence (Ex. 485 nm/Em. 535 nm).
Statistics and reproducibility
All biochemical experiments were performed in biological triplicate, except for the ATPase assay presented in Supplementary Fig. 6b, which was performed in technical triplicate. All replicates were successful, and no data were excluded from the analyses. In Figures, bars represent the mean and error bars the standard deviation from triplicate measurements. No statistical method was used to predetermine sample size. The experiments were not randomized, and the investigators were not blinded to allocation during experiments and outcome assessment.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
All information required to reanalyze the data reported in this paper has been deposited in a Source Data File and in Supplementary Data 1 and 2. The List of Prokaryotic names with Standing in Nomenclature can be found under https://www.bacterio.net/. UniProt Accession numbers used in the analysis of this work are listed in Supplementary Data 1. PDB 6MAT was used for structural analysis. Source data are provided with this article. Source data are provided with this paper.
References
Epstein, W., Buurman, E., McLaggan, D. & Naprstek, J. Multiple mechanisms, roles and controls of K+ transport in Escherichia coli. Biochem. Soc. Trans. 21 1006–1010 (1993).
Stautz, J. et al. Molecular mechanisms for bacterial potassium homeostasis. J. Mol. Biol. 433, 166968 (2021).
Stock, C. et al. Fast-forward on P-type ATPases: Recent advances on structure and function. Biochem Soc. Trans. 51, 1347–1360 (2023).
Altendorf, K. et al. Structure and function of the Kdp-ATPase of Escherichia coli. Acta Physiol. Scand. Suppl. 643, 137–146 (1998).
Voelkner, P., Puppe, W. & Altendorf, K. Characterization of the KdpD protein, the sensor kinase of the K(+)-translocating Kdp system of Escherichia coli. Eur. J. Biochem 217, 1019–1026 (1993).
Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P. & Altendorf, K. The sensor kinase KdpD of Escherichia coli senses external K+. Mol. Microbiol 88, 1194–1204 (2013).
Schramke, H., Tostevin, F., Heermann, R., Gerland, U. & Jung, K. A dual-sensing receptor confers robust cellular homeostasis. Cell Rep. 16, 213–221 (2016).
Walderhaug, M. O. et al. KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators. J. Bacteriol. 174, 2152–2159 (1992).
Altendorf, K., Voelkner, P. & Puppe, W. The sensor kinase KdpD and the response regulator KdpE control expression of the kdpFABC operon in Escherichia coli. Res Microbiol 145, 374–381 (1994).
Jung, K., Tjaden, B. & Altendorf, K. Purification, reconstitution, and characterization of KdpD, the turgor sensor of Escherichia coli. J. Biol. Chem. 272, 10847–10852 (1997).
Xie, M., Wu, M. & Han, A. Structural insights into the signal transduction mechanism of the K+-sensing two-component system KdpDE. Sci. Signal 13, 2970 (2020).
Roe, A. J., McLaggan, D., O’Byrne, C. P. & Booth, I. R. Rapid inactivation of the Escherichia coli Kdp K+ uptake system by high potassium concentrations. Mol. Microbiol 35, 1235–1243 (2000).
Huang, C.-S., Panyella Pedersen, B. & Stokes, D. L. Crystal structure of the potassium-importing KdpFABC membrane complex. Nature 546, 681–685 (2017).
Sweet, M. E. et al. Serine phosphorylation regulates the P-type potassium pump KdpFABC. eLife 9, e55480 (2020).
Silberberg, J. M. et al. Inhibited KdpFABC transitions into an E1 off-cycle state. eLife 11, e80988 (2022).
Mijakovic, I., Grangeasse, C. & Turgay, K. Exploring the diversity of protein modifications: special bacterial phosphorylation systems. FEMS Microbiol Rev. 40, 398–417 (2016).
Pereira, S. F. F., Goss, L. & Dworkin, J. Eukaryote-like serine/threonine kinases and phosphatases in bacteria. Microbiol Mol. Biol. Rev. 75, 192 (2011).
Janczarek, M., Vinardell, J. M., Lipa, P. & Karaś, M. Hanks-type serine/threonine protein kinases and phosphatases in bacteria: roles in signaling and adaptation to various environments. Int. J. Mol. Sci. 19, 2872 (2018).
Hanks, S. K., Quinn, A. M. & Hunter, T. The protein kinase family: Conserved features and deduced phylogeny of the catalytic domains. Science (1979) 241, 42–52 (1988).
Lilge, L. et al. The Involvement of the McsB arginine kinase in Clp-Dependent degradation of the MgsR regulator in Bacillus subtilis. Front Microbiol 11, 519873 (2020).
Dutta, R., Qin, L. & Inouye, M. Histidine kinases: diversity of domain organization. Mol. Microbiol 34, 633–640 (1999).
Grangeasse, C., Cozzone, A. J., Deutscher, J. & Mijakovic, I. Tyrosine phosphorylation: an emerging regulatory device of bacterial physiology. Trends Biochem. Sci. 32, 86–94 (2007).
Deutscher, J. & Saier, M. H. ATP-dependent protein kinase-catalyzed phosphorylation of a seryl residue in HPr, a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes. Proc. Natl. Acad. Sci. USA 80, 6790–6794 (1983).
Nimmo, G. A. & Nimmo, H. G. The regulatory properties of isocitrate dehydrogenase kinase and isocitrate dehydrogenase phosphatase from Escherichia coli ML308 and the roles of these activities in the control of isocitrate dehydrogenase. Eur. J. Biochem 141, 409–414 (1984).
Soares, N. C., Spät, P., Krug, K. & Macek, B. Global dynamics of the Escherichia coli proteome and phosphoproteome during growth in minimal medium. J. Proteome Res 12, 2611–2621 (2013).
Macek, B. et al. Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteom. 7, 299–307 (2008).
Stock, C. et al. Cryo-EM structures of KdpFABC suggest a K+ transport mechanism via two inter-subunit half-channels. Nat. Commun. 9, 4971 (2018).
Silberberg, J. M. et al. Deciphering ion transport and ATPase coupling in the intersubunit tunnel of KdpFABC. Nat. Commun. 12, 5098 (2021).
Haupt, M. et al. The holo-form of the nucleotide binding domain of the KdpFABC complex from Escherichia coli reveals a new binding mode. J. Biol. Chem. 281, 9641–9649 (2006).
Arifuzzaman, M. et al. Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16, 686–691 (2006).
Heermann, R., Altendorf, K. & Jung, K. The hydrophilic N-terminal domain complements the membrane-anchored C-terminal domain of the sensor kinase KdpD of Escherichia coli. J. Biol. Chem. 275, 17080–17085 (2000).
Jung, K. & Altendorf, K. Truncation of amino acids 12-128 causes deregulation of the phosphatase activity of the sensor kinase KdpD of Escherichia coli. J. Biol. Chem. 273, 17406–17410 (1998).
Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1, 945 (1982).
Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583–589 (2021).
Varadi, M. et al. AlphaFold protein structure database: Massively expanding the structural coverage of protein-sequence space with high-accuracy models. Nucleic Acids Res 50, D439–D444 (2022).
Iyer, L. M., Leipe, D. D., Koonin, E. V. & Aravind, L. Evolutionary history and higher order classification of AAA+ ATPases. J. Struct. Biol. 146, 11–31 (2004).
Hanson, P. I. & Whiteheart, S. W. AAA+ proteins: Have engine, will work. Nat. Rev. Mol. Cell Biol. 2005 6:7 6, 519–529 (2005).
Wang, X. et al. A c-di-AMP riboswitch controlling kdpFABC operon transcription regulates the potassium transporter system in Bacillus thuringiensis. Commun. Biol. 2, 151 (2019).
Parte, A. C., Carbasse, J. S., Meier-Kolthoff, J. P., Reimer, L. C. & Göker, M. List of prokaryotic names with standing in nomenclature (LPSN) moves to the DSMZ. Int J. Syst. Evol. Microbiol 70, 5607–5612 (2020).
Stülke, J. & Krüger, L. Cyclic di-AMP signaling in bacteria. Annu Rev. Microbiol 74, 159–179 (2020).
Moscoso, J. A. et al. Binding of Cyclic Di-AMP to the Staphylococcus aureus Sensor Kinase KdpD occurs via the universal stress protein domain and downregulates the expression of the Kdp potassium transporter. J. Bacteriol. 198, 98 (2016).
Dutta, A., Batish, M. & Parashar, V. Structural basis of KdpD histidine kinase binding to the second messenger c-di-AMP. J. Biol. Chem. 296, 100771 (2021).
Gundlach, J. et al. Control of potassium homeostasis is an essential function of the second messenger cyclic di-AMP in Bacillus subtilis. Sci. Signal 10, eaal3011 (2017).
Heermann, R. et al. The universal stress protein UspC scaffolds the KdpD/KdpE signaling cascade of Escherichia coli under salt stress. J. Mol. Biol. 386, 134–148 (2009).
Babu, M. et al. Global landscape of cell envelope protein complexes in Escherichia coli. Nat. Biotechnol. 36, 103–112 (2018).
Kipschull, K. Die Sensorkinase KdpD aus Escherichia coli: Funktionelle Reinigung, Nukleotidbindestudien und in vivo Protein-Protein Interaktionen. (University of Osnabrück, Osnabrück, 2011). https://osnadocs.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2012051010154.
Schniederberend, M., Zimmann, P., Bogdanov, M., Dowhan, W. & Altendorf, K. Influence of K+-dependent membrane lipid composition on the expression of the kdpFABC operon in Escherichia coli. Biochim. Biophys. Acta Biomembr. 1798, 32–39 (2010).
Stallkamp, I., Dowhan, W., Altendorf, K. & Jung, K. Negatively charged phospholipids influence the activity of the sensor kinase KdpD of Escherichia coli. Arch. Microbiol 172, 295–302 (1999).
Bai, Y. et al. Cyclic Di-AMP impairs potassium uptake mediated by a Cyclic Di-AMP binding protein in Streptococcus pneumoniae. J. Bacteriol. 196, 614 (2014).
Gundlach, J. et al. Adaptation of Bacillus subtilis to life at extreme potassium limitation. mBio 8, e00861–17 (2017).
Fuss, M. F. et al. Cyclic di-AMP traps proton-coupled K+ transporters of the KUP family in an inward-occluded conformation. Nat. Commun. 14, 3683 (2023).
Tascón, I. et al. Structural basis of proton-coupled potassium transport in the KUP family. Nat. Commun. 11, 626 (2020).
Nelson, J. W. et al. Riboswitches in eubacteria sense the second messenger c-di-AMP. Nat. Chem. Biol. 9, 834–839 (2013).
Dani, P., Kumar Ujaoney, A., Apte, S. K. & Basu, B. Regulation of potassium dependent ATPase (kdp) operon of Deinococcus radiodurans. PLoS One 12, e0188998 (2017).
Schleußinger, E., Schmid, R. & Bakker, E. P. New type of kdp region with a split sensor-kinase kdpD gene located within two divergent kdp operons from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius. Biochim. Biophys. Acta 1759, 437–441 (2006).
Ballal, A. & Apte, S. K. Differential expression of the two kdp operons in the nitrogen-fixing cyanobacterium Anabaena sp. strain L-31. Appl. Environ. Microbiol 71, 5297–5303 (2005).
Corrigan, R. M. et al. Systematic identification of conserved bacterial c-di-AMP receptor proteins. Proc. Natl. Acad. Sci. 110, 9084–9089 (2013).
Dubnau, D. Genetic competence in Bacillus subtilis. Microbiol Rev. 55, 395–424 (1991).
Jung, K., Fried, L., Behr, S. & Heermann, R. Histidine kinases and response regulators in networks. Curr. Opin. Microbiol 15, 118–124 (2012).
Unden, G., Wörner, S. & Monzel, C. Cooperation of secondary transporters and sensor kinases in transmembrane signalling: The DctA/DcuS and DcuB/DcuS sensor complexes of Escherichia coli. Adv. Micro. Physiol. 68, 139–167 (2016).
Piepenbreier, H., Fritz, G. & Gebhard, S. Transporters as information processors in bacterial signalling pathways. Mol. Microbiol 104, 1–15 (2017).
Rietkötter, E., Hoyer, D. & Mascher, T. Bacitracin sensing in Bacillus subtilis. Mol. Microbiol 68, 768–785 (2008).
Fritz, G. et al. A new way of sensing: Need-based activation of antibiotic resistance by a flux-sensing mechanism. mBio 6, e00975 (2015).
Hsieh, Y. J. & Wanner, B. L. Global regulation by the seven-component Pi signaling system. Curr. Opin. Microbiol 13, 198–203 (2010).
Joly, N., Böhm, A., Boos, W. & Richet, E. MalK, the ATP-binding cassette component of the Escherichia coli maltodextrin transporter, inhibits the transcriptional activator MalT by antagonizing inducer binding. J. Biol. Chem. 279, 33123–33130 (2004).
Charles, T. C. & Nester, E. W. A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens. J. Bacteriol. 175, 6614–6625 (1993).
Mattoo, A. R. et al. Spo0B of Bacillus anthracis - a protein with pleiotropic functions. FEBS J. 275, 739–752 (2008).
Geertsma, E. R. & Dutzler, R. A versatile and efficient high-throughput cloning tool for structural biology. Biochemistry 50, 3272–3278 (2011).
Kunkel, T. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82, 488–492 (1985).
Stumpe, S. & Bakker, E. P. Requirement of a large K+ -uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch. Microbiol 167, 126–136 (1997).
Puppe, W., Zimmann, P., Jung, K., Lucassen, M. & Altendorf, K. Characterization of truncated Forms of the KdpD protein, the sensor kinase of the K+-translocating Kdp system of Escherichia coli. J. Biol. Chem. 271, 25027–25034 (1996).
Carter, S. G. & Karl, D. W. Inorganic phosphate assay with malachite green: An improvement and evaluation. J. Biochem. Biophys. Methods 7, 7–13 (1982).
Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970).
Sievers, F. et al. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol. Syst. Biol. 7, 539 (2011).
Lo, Y. H. et al. Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7. Nat. Commun. 10, 513 (2019).
Acknowledgements
This work was funded by the State of Hesse in the LOEWE Schwerpunkt TRABITA, a DFG Heisenberg grant (HA6322/5-1), and funding from the Aventis Foundation and the Uniscientia Stiftung.
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J.M.S.: Conceptualization, Validation, Formal analysis, Investigation, Writing—original draft, Writing—review & editing, Visualization. S.K.: Investigation, Formal analysis, Writing—review & editing. P.J.N.B.: Investigation, Formal analysis, Writing—review & editing. K.J.: Investigation, Formal analysis. M.W.: Investigation. J.G.: Investigation. I.H.: Conceptualization, Writing—review & editing, Supervision, Project administration, Funding acquisition.
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Silberberg, J.M., Ketter, S., Böhm, P.J.N. et al. KdpD is a tandem serine histidine kinase that controls K+ pump KdpFABC transcriptionally and post-translationally. Nat Commun 15, 3223 (2024). https://doi.org/10.1038/s41467-024-47526-8
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DOI: https://doi.org/10.1038/s41467-024-47526-8
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